Bio-Ocean

ELISA/assay

Rat IL-10 ELISA Kit

EK310

96 well test

349 USD

EK310

Rat IL-10 ELISA Kit

Rat

ELISA

96 well test

349 USD

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IL-10. The Rat IL-10 ELISA is for research use only. Not for use in therapeutic procedures. Rat Interleukin 10 (IL-10) cDNA encodes a 178 amino acid (aa) residue precursor protein that contains an 18 aa signal sequence and a 160 aa mature protein. Mature rat IL-10 shares 85% and 74% aa sequence identity with mouse and human IL-10, respectively. IL-10 is primarily produced by monocytes and, to a lesser extent, lymphocytes, namely type 2 T helper cells (Th2), mastocytes, CD4+CD25+Foxp3+ regulatory T cells, and in a certain subset of activated T cells and B cells. IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.