product summary
company name :
Badrilla
product type :
antibody
product name :
 RYR2 Phospho Ser-2814 Anti-Serum
catalog :
A010-31
quantity :
50 ul
price :
GBP356.42
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - frozen section
more info or order :
Badrilla product webpage
citations: 4
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; fig 2
Asensio-López M, Soler F, Sánchez-Más J, Pascual-Figal D, Fernandez-Belda F, Lax A. Early oxidative damage induced by doxorubicin: Source of production, protection by GKT137831 and effect on Ca(2+) transporters in HL-1 cardiomyocytes. Arch Biochem Biophys. 2016;594:26-36 pubmed publisher
  • western blot; rat; 1:5000; fig s1
Sadredini M, Danielsen T, Aronsen J, Manotheepan R, Hougen K, Sjaastad I, et al. Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure. PLoS ONE. 2016;11:e0153887 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100
Kim E, Shekhar A, Lu J, Lin X, Liu F, Zhang J, et al. PCP4 regulates Purkinje cell excitability and cardiac rhythmicity. J Clin Invest. 2014;124:5027-36 pubmed publisher
Besser J, Malan D, Wystub K, Bachmann A, Wietelmann A, Sasse P, et al. MiRNA-1/133a clusters regulate adrenergic control of cardiac repolarization. PLoS ONE. 2014;9:e113449 pubmed publisher
product information
Catalog Number :
A010-31
Product Name :
 RYR2 Phospho Ser-2814 Anti-Serum
Host Species :
Rabbit
Clonality :
polyclonal
Antigen Modification :
Phospho-Serine
Product Type :
Polyclonal Ab
Size :
50 ul
List Price :
GBP356.42
Gene ID :
6262
Product Description :
Rabbit Polyclonal anti-Serum specific to Ser-2814 phosphorylated RYR2.
CrossReactivity :
Recognises Ser-2814 phosphorylated RyR2 exclusively, and will not react with dephosphorylated RyR2 or RyR2 phosphorylated at other sites.
SpeciesSummary :
Peptide aligns with RyR2 from all mammalian species. Sequence antibody recognises both sequences.corresponds exactly in rabbit but differs at one residue (V2815I) in mouse and rat. However
Background :
The ryanodine receptor (RyR2) is a Ca2+ channel of cardiac muscle that plays a central role in EC coupling. The binding of Ca2+ to RyR2 opens the channel and Ca2+ stored in the SR moves through the channel into the cytosol to initiate muscle contraction. CaMKII, was able to phosphorylate Ser-2814 of RYR2 enhancing Ca2+- sensitivity and increasing open probability. Excessive phosphorylation of Ser-2814 leads to atrial fibrillation, arrhythmias and sudden death.
Immunogen :
Synthetic peptide (TSQVS(PO3H2)VDAAH2819) corresponding to amino acids surrounding the phosphorylated serine residue at position 2814 of RyR2 (human) conjugated to KLH
Specificity :
Recognises Ser-2814 phosphorylated RyR2 exclusively, and will not react with dephosphorylated RyR2 or RyR2 phosphorylated at other sites.
Purity :
Raw Serum
Preservative :
Lyophilised:
Packaging :
0.5 ml Cryovial
Uses :
WB, IHC
Application Summary :
 Western blot (1:5000 dilution), IHC Microscopy (1:100 dilution)
Storage :
Lyophilised antibody is stable at 4 °C when stored with desiccant. Reconstitute lyophilised powder in 50 ul of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freese - thaw cycles
Product References :
Bers, D. M. (2002) Nature 415, 198-205
more info or order :
Badrilla product webpage
company information
Badrilla
Leeds Innovation Centre
103 Clarendon Road
Leeds, LS2 9DF
info@badrilla.com
www.badrilla.com
011334 31869
headquarters: UK
Badrilla Ltd. is a biotechnology company founded in 2003 and located in Leeds, UK. Badrilla makes and distributes exceptional monoclonal and polyclonal antibodies to cardiovascular protein and phospho-protein targets. We source excellent antibodies from academic leaders and re-validate antibodies not made by ourselves in assays of relevance to our customers. Working with outstanding academic collaborators, we are developing new technologies to calibrate immunoassays, which will enable the performance of genuinely Quantitative Western blotting, Quantitative biomarker measurement, and Quantitative diagnostics. Our aim is to provide products that work first time in the applications you deploy, which accelerate the progress of your research, and which satisfy your expectations in every way.