product summary
company name :
Badrilla
product type :
antibody
product name :
RYR2 Phospho Serine-2808 Anti-Serum
catalog :
A010-30
quantity :
50 ul
price :
GBP356.42
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, mouse, rat, dog, pig
application :
western blot, ELISA, immunohistochemistry
more info or order :
Badrilla product webpage
citations: 9
Published Application/Species/Sample/DilutionReference
  • western blot; rat; 1:5000; fig s1
Sadredini M, Danielsen T, Aronsen J, Manotheepan R, Hougen K, Sjaastad I, et al. Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure. PLoS ONE. 2016;11:e0153887 pubmed publisher
  • western blot; mouse; loading ...; fig 7f
Mederle K, Gess B, Pluteanu F, Plackic J, Tiefenbach K, Grill A, et al. The angiotensin receptor-associated protein Atrap is a stimulator of the cardiac Ca2+-ATPase SERCA2a. Cardiovasc Res. 2016;110:359-70 pubmed publisher
  • western blot; mouse; fig 2
Asensio López M, Soler F, Sánchez Más J, Pascual Figal D, Fernández Belda F, Lax A. Early oxidative damage induced by doxorubicin: Source of production, protection by GKT137831 and effect on Ca(2+) transporters in HL-1 cardiomyocytes. Arch Biochem Biophys. 2016;594:26-36 pubmed publisher
  • western blot; human; 1:5000; fig 4
Rapti K, Stillitano F, Karakikes I, Nonnenmacher M, Weber T, Hulot J, et al. Effectiveness of gene delivery systems for pluripotent and differentiated cells. Mol Ther Methods Clin Dev. 2015;2:14067 pubmed publisher
  • western blot; pig
Lugenbiel P, Wenz F, Govorov K, Schweizer P, Katus H, Thomas D. Atrial fibrillation complicated by heart failure induces distinct remodeling of calcium cycling proteins. PLoS ONE. 2015;10:e0116395 pubmed publisher
  • western blot; mouse
Yi T, Vick J, Vecchio M, Begin K, Bell S, Delay R, et al. Identifying cellular mechanisms of zinc-induced relaxation in isolated cardiomyocytes. Am J Physiol Heart Circ Physiol. 2013;305:H706-15 pubmed publisher
Brandenburg S, Pawlowitz J, Fakuade F, Kownatzki Danger D, Kohl T, Mitronova G, et al. Axial Tubule Junctions Activate Atrial Ca2+ Release Across Species. Front Physiol. 2018;9:1227 pubmed publisher
Wilder C, Pavlaki N, Dursun T, Gyimah P, Caldwell Dunn E, Ranieri A, et al. Facilitation of ischaemia-induced ventricular fibrillation by catecholamines is mediated by β1 and β2 agonism in the rat heart in vitro. Br J Pharmacol. 2018;175:1669-1690 pubmed publisher
Besser J, Malan D, Wystub K, Bachmann A, Wietelmann A, Sasse P, et al. MiRNA-1/133a clusters regulate adrenergic control of cardiac repolarization. PLoS ONE. 2014;9:e113449 pubmed publisher
product information
Catalog Number :
A010-30
Product Name :
RYR2 Phospho Serine-2808 Anti-Serum
Host Species :
Rabbit
Clonality :
polyclonal
Antigen Modification :
Phospho-Serine
Product Type :
Polyclonal Ab
Size :
50 ul
List Price :
GBP356.42
Gene ID :
6262
Product Description :
Rabbit Polyclonal anti-serum specific to RYR2 phospho-Ser2808
CrossReactivity :
The antibody recognises phosphorylated Serine-2808 of the ryanodine peptide containing the Phospho Ser-2808 epitope.receptor and binding is blocked in the presence of a
SpeciesSummary :
human, rat, mouse, rabbit, dog, canine and sheep
Background :
The ryanodine receptor (RyR2) is a Ca2+ channel of cardiac muscle that plays a central role in EC coupling. The binding of Ca2+  to RyR2 opens the channel and Ca2+ stored in the SR moves through the channel into the cytosol to initiate muscle contraction. Abnormal structure and function of ryanodine receptors has been reported in failing hearts, with Ser-2808 phosphorylation appearing elevated in clinical situations which may contribute to the abnormal Ca2+ handling characteristics of cardiac muscle in these conditions. Ser-2808 can be phosphorylated in vitro by PKA or CaMKII, which is coincident with significant change in RyR2 channel function typified by an increased open probability, the abrogation of the inhibitory effects of CaM, and Mg2+, dissociation of regulatory factors, expression of subconductance states and the expression of channel activity at diastolic Ca2+ concentrations. Serine-2808 in human RyR2 equates to Ser-2809 in rabbit RyR2.
Immunogen :
Synthetic peptide (YNRTRRIS(PO3H2)QT2810) conjugated to keyhole limpet haemocyanin
Specificity :
The antibody recognises phosphorylated Serine-2808 of the ryanodine peptide containing the Phospho Ser-2808 epitope.receptor and binding is blocked in the presence of a
Purity :
Raw Serum
Preservative :
Lyophilised:
Packaging :
0.5 ml Cryovial
Uses :
WB, ELISA, IHC
Application Summary :
WB (1:5000), IHC (1:100), ELISA
Storage :
Lyophilised antibody is stable at 4 °C when stored with desiccant. Reconstitute lyophilised powder in 50 ul of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freese - thaw cycles
Product References :
Carter, S., Colyer, J., & Sitsapesan, R. (2006) Circ. Res. 98, 1506-13
more info or order :
Badrilla product webpage
company information
Badrilla
Leeds Innovation Centre
103 Clarendon Road
Leeds, LS2 9DF
info@badrilla.com
http://www.badrilla.com
011334 31869
headquarters: UK
Badrilla Ltd. is a biotechnology company founded in 2003 and located in Leeds, UK. Badrilla makes and distributes exceptional monoclonal and polyclonal antibodies to cardiovascular protein and phospho-protein targets. We source excellent antibodies from academic leaders and re-validate antibodies not made by ourselves in assays of relevance to our customers. Working with outstanding academic collaborators, we are developing new technologies to calibrate immunoassays, which will enable the performance of genuinely Quantitative Western blotting, Quantitative biomarker measurement, and Quantitative diagnostics. Our aim is to provide products that work first time in the applications you deploy, which accelerate the progress of your research, and which satisfy your expectations in every way.