Phospholamban Phospho Threonine-17 Anti-Serum | Badrilla A010-13 product information

product summary

company name :

Badrilla

product type :

antibody

product name :

Phospholamban Phospho Threonine-17 Anti-Serum

catalog :

A010-13

quantity :

20 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat, domestic rabbit

application :

western blot, immunohistochemistry

more info or order :

Badrilla product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 3d	Neumann J, Grobe J, Weisgut J, Schwelberger H, Fogel W, Marusakova M, et al. Histamine can be Formed and Degraded in the Human and Mouse Heart. Front Pharmacol. 2021;12:582916 pubmed publisher
western blot; mouse; 1:5000; loading ...; fig 5a	Dai W, Laforest B, Tyan L, Shen K, Nadadur R, Alvarado F, et al. A calcium transport mechanism for atrial fibrillation in Tbx5-mutant mice. elife. 2019;8: pubmed publisher
western blot; domestic rabbit; 1:5000; loading ...; fig 5	Chang S, Chuang H, Chen Y, Kao Y, Lin Y, Yeh Y, et al. Heart failure modulates electropharmacological characteristics of sinoatrial nodes. Exp Ther Med. 2017;13:771-779 pubmed publisher
western blot; mouse; loading ...; fig 6c	Kennedy T, Swiderski K, Murphy K, Gehrig S, Curl C, Chandramouli C, et al. BGP-15 Improves Aspects of the Dystrophic Pathology in mdx and dko Mice with Differing Efficacies in Heart and Skeletal Muscle. Am J Pathol. 2016;186:3246-3260 pubmed publisher
western blot; rat; 1:5000; fig s1	Sadredini M, Danielsen T, Aronsen J, Manotheepan R, Hougen K, Sjaastad I, et al. Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure. PLoS ONE. 2016;11:e0153887 pubmed publisher
western blot; mouse; fig 3	Shimura D, Kusakari Y, Sasano T, Nakashima Y, Nakai G, Jiao Q, et al. Heterozygous deletion of sarcolipin maintains normal cardiac function. Am J Physiol Heart Circ Physiol. 2016;310:H92-103 pubmed publisher
	Zhong P, Quan D, Huang Y, Huang H. CaMKII Activation Promotes Cardiac Electrical Remodeling and Increases the Susceptibility to Arrhythmia Induction in High-fat Diet-Fed Mice With Hyperlipidemia Conditions. J Cardiovasc Pharmacol. 2017;70:245-254 pubmed publisher
	Helms A, Alvarado F, Yob J, Tang V, Pagani F, Russell M, et al. Genotype-Dependent and -Independent Calcium Signaling Dysregulation in Human Hypertrophic Cardiomyopathy. Circulation. 2016;134:1738-1748 pubmed

product information

Catalog Number :

A010-13

Product Name :

Phospholamban Phospho Threonine-17 Anti-Serum

Host Species :

Rabbit

Clonality :

polyclonal

Antigen Modification :

Phospho-Threonine

Product Type :

Polyclonal Ab

Size :

20 ul

Gene ID :

5350

Product Rank :

Product Description :

Rabbit Polyclonal Anti-Serum specific to Thr-17 phosphorylated forms of Phospholamban.

SpeciesSummary :

All mammalian species, when phosphorylated on Thr-17

Background :

Phospholamban (PLB/PLN) is a small transmembrane protein which plays an important role in controlling the activity of the sarcoplasmic reticulum ATPase (SERCA2a) of cardiac muscle during calcium sequestration. Phospholamban is phosphorylated on separate amino acid residues by cAMP-dependent, and cGMP-dependent (Ser-16) and Ca2+/CaM-dependent (Thr-17) protein kinases in response to beta-adrenergic stimulation. Akt has also been shown to phosphorylate Thr-17. The result is an increased calcium pump activity which reduces the time course of the calcium transient, increases the calcium load in the sarcoplasmic reticulum, and consequently, produces a larger calcium transient at the next action potential. However, alteration in this homeostatic interaction has been shown to result in heart failure.

Immunogen :

Phosphopeptide comprising residues 9-19-Y (residues R9SAIRRAST(PO3H2)IEY20) conjugated to KLH.

Specificity :

The antibody recognises mono and oligomeric phospholamban when phosphorylated on threonine-17. Binding of the antibody to its target epitope is blocked in the epitope. presence of a phosphopeptide containing the PLB Phospho Thr-17

Purity :

Raw Serum

Preservative :

Lyophilised

Packaging :

0.5 ml Cryovial

Uses :

WB, ELISA, IHC

Application Summary :

WB: (1:5000), IHC: (1:200)

Storage :

Lyophilised antibody is stable at 4 °C when stored with desiccant. Reconstitute lyophilised powder in 20 ul of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freese - thaw cycles

Product References :

Drago, G. A., and Colyer, J. (1994) J Biol Chem 269, 25073-25077

more info or order :

Badrilla product webpage