Phospholamban Phospho Serine-16 | Badrilla A010-12 product information

product summary

company name :

Badrilla

product type :

antibody

product name :

Phospholamban Phospho Serine-16

catalog :

A010-12

quantity :

20 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Badrilla product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 3d	Neumann J, Grobe J, Weisgut J, Schwelberger H, Fogel W, Marusakova M, et al. Histamine can be Formed and Degraded in the Human and Mouse Heart. Front Pharmacol. 2021;12:582916 pubmed publisher
western blot; mouse; 1:5000; loading ...; fig 5a	Dai W, Laforest B, Tyan L, Shen K, Nadadur R, Alvarado F, et al. A calcium transport mechanism for atrial fibrillation in Tbx5-mutant mice. elife. 2019;8: pubmed publisher
western blot; rat; 1:5000; loading ...; fig 2c	Scotcher J, Prysyazhna O, Boguslavskyi A, Kistamás K, Hadgraft N, Martin E, et al. Disulfide-activated protein kinase G I? regulates cardiac diastolic relaxation and fine-tunes the Frank-Starling response. Nat Commun. 2016;7:13187 pubmed publisher
western blot; rat; 1:5000; fig s1	Sadredini M, Danielsen T, Aronsen J, Manotheepan R, Hougen K, Sjaastad I, et al. Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure. PLoS ONE. 2016;11:e0153887 pubmed publisher
western blot; mouse; loading ...; fig 7d	Mederle K, Gess B, Pluteanu F, Plackic J, Tiefenbach K, Grill A, et al. The angiotensin receptor-associated protein Atrap is a stimulator of the cardiac Ca2+-ATPase SERCA2a. Cardiovasc Res. 2016;110:359-70 pubmed publisher
western blot; mouse; fig 3	Shimura D, Kusakari Y, Sasano T, Nakashima Y, Nakai G, Jiao Q, et al. Heterozygous deletion of sarcolipin maintains normal cardiac function. Am J Physiol Heart Circ Physiol. 2016;310:H92-103 pubmed publisher
	Subramanian H, Froese A, Jonsson P, Schmidt H, Gorelik J, Nikolaev V. Distinct submembrane localisation compartmentalises cardiac NPR1 and NPR2 signalling to cGMP. Nat Commun. 2018;9:2446 pubmed publisher
	Kusakari Y, Urashima T, Shimura D, Amemiya E, Miyasaka G, Yokota S, et al. Impairment of Excitation-Contraction Coupling in Right Ventricular Hypertrophied Muscle with Fibrosis Induced by Pulmonary Artery Banding. PLoS ONE. 2017;12:e0169564 pubmed publisher
	Helms A, Alvarado F, Yob J, Tang V, Pagani F, Russell M, et al. Genotype-Dependent and -Independent Calcium Signaling Dysregulation in Human Hypertrophic Cardiomyopathy. Circulation. 2016;134:1738-1748 pubmed

product information

Catalog Number :

A010-12

Product Name :

Phospholamban Phospho Serine-16

Host Species :

Rabbit

Clonality :

polyclonal

Antigen Modification :

Phospho-Serine

Product Type :

Polyclonal Ab

Size :

20 ul

Gene ID :

5350

Product Rank :

Product Description :

Rabbit polyclonal antibody to phospholamban phospho Serine-16.

SpeciesSummary :

All mammalian species, when phosphorylated on Ser-16.

Background :

Phospholamban (PLB/PLN) is a small transmembrane protein which plays an important role in controlling the activity of the sarcoplasmic reticulum ATPase (SERCA2a) of cardiac muscle during calcium sequestration. Phospholamban is phosphorylated on separate amino acid residues by cAMP-dependent, and cGMP-dependent (Ser-16) and Ca2+/CaM-dependent (Thr-17) protein kinases in response to beta-adrenergic stimulation. Akt has also been shown to phosphorylate Thr-17. The result is an increased calcium pump activity which reduces the time course of the calcium transient, increases the calcium load in the sarcoplasmic reticulum, and consequently, produces a larger calcium transient at the next action potential. However, alteration in this homeostatic interaction has been shown to result in heart failure

Immunogen :

Phosphopeptide comprising residues 9-19-Y (residues R9SAIRRAS(PO3H2)TIE19Y) conjugated to KLH

Specificity :

All mammalian species, when phosphorylated on Ser-16.

Purity :

Raw Serum

Preservative :

Lyophilised: Contains no preservatives.

Packaging :

0.5 ml Cryovial

Uses :

WB, ELISA, IHC

Application Summary :

WB: (1:5000)

Storage :

Lyophilised antibody is stable at 4 °C when stored with desiccant. Reconstitute lyophilised powder in 20 ul of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freese - thaw cycles

Product References :

Drago, G. A., and Colyer, J. (1994) J Biol Chem 269, 25073-25077

more info or order :

Badrilla product webpage