product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
Nestin
catalog :
611658
quantity :
50 µg
price :
207.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
25/NESTIN
reactivity :
human, mouse, rat, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation, western blot knockout validation
more info or order :
citations: 27
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; 1:1000; loading ...; fig s2f
Zhang Y, Wang J, Huang W, Cai J, Ba J, Wang Y, et al. Nuclear Nestin deficiency drives tumor senescence via lamin A/C-dependent nuclear deformation. Nat Commun. 2018;9:3613 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig 4a
Jin J, Ravindran P, Di Meo D, Püschel A. Igf1R/InsR function is required for axon extension and corpus callosum formation. PLoS ONE. 2019;14:e0219362 pubmed publisher
  • immunocytochemistry; human; 1:150; loading ...; fig s5e
  • western blot; human; 1:1000; loading ...; fig 5e
Zhou A, Lin K, Zhang S, Chen Y, Zhang N, Xue J, et al. Nuclear GSK3β promotes tumorigenesis by phosphorylating KDM1A and inducing its deubiquitylation by USP22. Nat Cell Biol. 2016;18:954-966 pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; fig 1d
Long Y, Xu M, Li R, Dai S, Beers J, Chen G, et al. Induced Pluripotent Stem Cells for Disease Modeling and Evaluation of Therapeutics for Niemann-Pick Disease Type A. Stem Cells Transl Med. 2016;5:1644-1655 pubmed
  • immunocytochemistry; human; 1:500; fig 2
Momcilovic O, Sivapatham R, Oron T, Meyer M, Mooney S, Rao M, et al. Derivation, Characterization, and Neural Differentiation of Integration-Free Induced Pluripotent Stem Cell Lines from Parkinson's Disease Patients Carrying SNCA, LRRK2, PARK2, and GBA Mutations. PLoS ONE. 2016;11:e0154890 pubmed publisher
  • immunohistochemistry; mouse; 1:200; fig 3
Shah B, Lutter D, Bochenek M, Kato K, Tsytsyura Y, Glyvuk N, et al. C3G/Rapgef1 Is Required in Multipolar Neurons for the Transition to a Bipolar Morphology during Cortical Development. PLoS ONE. 2016;11:e0154174 pubmed publisher
  • immunocytochemistry; rat; 1:1000; fig 2
  • western blot; rat; 1:1000; fig 3
Chao C, Kan D, Lo T, Lu K, Chien C. Induction of neural differentiation in rat C6 glioma cells with taxol. Brain Behav. 2015;5:e00414 pubmed publisher
  • immunocytochemistry; mouse
Lebkuechner I, Wilhelmsson U, Möllerström E, Pekna M, Pekny M. Heterogeneity of Notch signaling in astrocytes and the effects of GFAP and vimentin deficiency. J Neurochem. 2015;135:234-48 pubmed publisher
  • western blot; human; 1:250
Jha B, Rao M, Malik N. Motor neuron differentiation from pluripotent stem cells and other intermediate proliferative precursors that can be discriminated by lineage specific reporters. Stem Cell Rev. 2015;11:194-204 pubmed publisher
  • western blot; human; fig 1d
Gasimli L, Hickey A, Yang B, Li G, dela Rosa M, Nairn A, et al. Changes in glycosaminoglycan structure on differentiation of human embryonic stem cells towards mesoderm and endoderm lineages. Biochim Biophys Acta. 2014;1840:1993-2003 pubmed publisher
  • immunohistochemistry; mouse; 1:500
Momcilovic O, Liu Q, Swistowski A, Russo Tait T, Zhao Y, Rao M, et al. Genome wide profiling of dopaminergic neurons derived from human embryonic and induced pluripotent stem cells. Stem Cells Dev. 2014;23:406-20 pubmed publisher
  • immunocytochemistry; human; 1:500
Liu Q, Pedersen O, Peng J, Couture L, Rao M, Zeng X. Optimizing dopaminergic differentiation of pluripotent stem cells for the manufacture of dopaminergic neurons for transplantation. Cytotherapy. 2013;15:999-1010 pubmed publisher
  • immunocytochemistry; mouse
Sparmann A, Xie Y, Verhoeven E, Vermeulen M, Lancini C, Gargiulo G, et al. The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of astroglial differentiation. EMBO J. 2013;32:1598-612 pubmed publisher
  • immunocytochemistry; human; 1:500
Liu Q, Spusta S, Mi R, Lassiter R, Stark M, Hoke A, et al. Human neural crest stem cells derived from human ESCs and induced pluripotent stem cells: induction, maintenance, and differentiation into functional schwann cells. Stem Cells Transl Med. 2012;1:266-78 pubmed publisher
  • chromatin immunoprecipitation; human
Darbinyan A, Kaminski R, White M, Darbinian Sarkissian N, Khalili K. Polyomavirus JC infection inhibits differentiation of oligodendrocyte progenitor cells. J Neurosci Res. 2013;91:116-27 pubmed publisher
  • western blot; zebrafish
Schneider L, d Adda di Fagagna F. Neural stem cells exposed to BrdU lose their global DNA methylation and undergo astrocytic differentiation. Nucleic Acids Res. 2012;40:5332-42 pubmed publisher
Kuang Y, Muñoz A, Nalula G, Santostefano K, Sanghez V, Sanchez G, et al. Evaluation of commonly used ectoderm markers in iPSC trilineage differentiation. Stem Cell Res. 2019;37:101434 pubmed publisher
Leferink P, Breeuwsma N, Bugiani M, van der Knaap M, Heine V. Affected astrocytes in the spinal cord of the leukodystrophy vanishing white matter. Glia. 2018;66:862-873 pubmed publisher
Hughes A, Spelke D, Xu Z, Kang C, Schaffer D, Herr A. Single-cell western blotting. Nat Methods. 2014;11:749-55 pubmed publisher
Schneider L. Survival of neural stem cells undergoing DNA damage-induced astrocytic differentiation in self-renewal-promoting conditions in vitro. PLoS ONE. 2014;9:e87228 pubmed publisher
Fu X, Rong Z, Zhu S, Wang X, Xu Y, Lake B. Genetic approach to track neural cell fate decisions using human embryonic stem cells. Protein Cell. 2014;5:69-79 pubmed publisher
Aldaz B, Sagardoy A, Nogueira L, Guruceaga E, Grande L, Huse J, et al. Involvement of miRNAs in the differentiation of human glioblastoma multiforme stem-like cells. PLoS ONE. 2013;8:e77098 pubmed publisher
Larsson H, Lee S, Roccio M, Velluto D, Lutolf M, Frey P, et al. Sorting live stem cells based on Sox2 mRNA expression. PLoS ONE. 2012;7:e49874 pubmed publisher
Babu H, Claasen J, Kannan S, Rünker A, Palmer T, Kempermann G. A protocol for isolation and enriched monolayer cultivation of neural precursor cells from mouse dentate gyrus. Front Neurosci. 2011;5:89 pubmed publisher
Swistowski A, Peng J, Liu Q, Mali P, Rao M, Cheng L, et al. Efficient generation of functional dopaminergic neurons from human induced pluripotent stem cells under defined conditions. Stem Cells. 2010;28:1893-904 pubmed publisher
Han Y, Miller A, Mangada J, Liu Y, Swistowski A, Zhan M, et al. Identification by automated screening of a small molecule that selectively eliminates neural stem cells derived from hESCs but not dopamine neurons. PLoS ONE. 2009;4:e7155 pubmed publisher
Swistowski A, Peng J, Han Y, Swistowska A, Rao M, Zeng X. Xeno-free defined conditions for culture of human embryonic stem cells, neural stem cells and dopaminergic neurons derived from them. PLoS ONE. 2009;4:e6233 pubmed publisher
product information
Cat.# :
611658
Brand :
BD Transduction Laboratories
Name :
Nestin
Format :
Purified
Clone :
25/NESTIN
Isotype :
Ms IgG 1
React :
Hu , Rat
Conc :
250 µg/ml
Apps :
WB
Size :
50 µg
Status :
RUO
List Price :
207.00 USD
more info or order :
company information