product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
GOK
catalog :
610954
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
44/GOK
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, proximity ligation assay, western blot knockout validation
more info or order :
citations: 23
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; fig u87
Xie J, Ma G, Zhou L, He L, Zhang Z, Tan P, et al. Identification of a STIM1 Splicing Variant that Promotes Glioblastoma Growth. Adv Sci (Weinh). 2022;9:e2103940 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1k
Kim J, Hwang K, Dang B, Eom M, Kong I, Gwack Y, et al. Insulin-activated store-operated Ca2+ entry via Orai1 induces podocyte actin remodeling and causes proteinuria. Nat Commun. 2021;12:6537 pubmed publisher
  • western blot; mouse; fig 4c
Ghosh A, Coakley R, Mascenik T, Rowell T, Davis E, Rogers K, et al. Chronic E-Cigarette Exposure Alters the Human Bronchial Epithelial Proteome. Am J Respir Crit Care Med. 2018;198:67-76 pubmed publisher
  • western blot; human; loading ...; fig 1b
Diez Bello R, Jardin I, Salido G, Rosado J. Orai1 and Orai2 mediate store-operated calcium entry that regulates HL60 cell migration and FAK phosphorylation. Biochim Biophys Acta Mol Cell Res. 2017;1864:1064-1070 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 1b
Oláh T, Bodnár D, Tóth A, Vincze J, Fodor J, Reischl B, et al. Cannabinoid signalling inhibits sarcoplasmic Ca2+ release and regulates excitation-contraction coupling in mammalian skeletal muscle. J Physiol. 2016;594:7381-7398 pubmed publisher
  • western blot; mouse; loading ...; fig 1a
Albarrán L, Regodón S, Salido G, Lopez J, Rosado J. Role of STIM1 in the surface expression of SARAF. Channels (Austin). 2017;11:84-88 pubmed publisher
  • proximity ligation assay; human; fig 3
  • immunoprecipitation; human; fig 1
  • western blot; human; fig s1
Albarrán L, Lopez J, Amor N, Martin Cano F, Berna Erro A, Smani T, et al. Dynamic interaction of SARAF with STIM1 and Orai1 to modulate store-operated calcium entry. Sci Rep. 2016;6:24452 pubmed publisher
  • immunoprecipitation; mouse; fig 2
De Mario A, Castellani A, Peggion C, Massimino M, Lim D, Hill A, et al. The prion protein constitutively controls neuronal store-operated Ca(2+) entry through Fyn kinase. Front Cell Neurosci. 2015;9:416 pubmed publisher
  • western blot; mouse; 1:500; fig 1d
Tóth A, Fodor J, Vincze J, Oláh T, Juhász T, Zákány R, et al. The Effect of SERCA1b Silencing on the Differentiation and Calcium Homeostasis of C2C12 Skeletal Muscle Cells. PLoS ONE. 2015;10:e0123583 pubmed publisher
  • western blot; human; fig 6
Sun J, Lu F, He H, Shen J, Messina J, Mathew R, et al. STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates invadopodium formation and melanoma invasion. J Cell Biol. 2014;207:535-48 pubmed publisher
  • western blot; human; fig 2
Makowski S, Wang Z, Pomerantz J. A protease-independent function for SPPL3 in NFAT activation. Mol Cell Biol. 2015;35:451-67 pubmed publisher
  • western blot; human; loading ...; fig 1c
Kondratska K, Kondratskyi A, Yassine M, Lemonnier L, Lepage G, Morabito A, et al. Orai1 and STIM1 mediate SOCE and contribute to apoptotic resistance of pancreatic adenocarcinoma. Biochim Biophys Acta. 2014;1843:2263-9 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:50
Cross B, Hack A, Reinhardt T, Rao R. SPCA2 regulates Orai1 trafficking and store independent Ca2+ entry in a model of lactation. PLoS ONE. 2013;8:e67348 pubmed publisher
  • western blot; human; 1:300
Thompson J, Shuttleworth T. Molecular basis of activation of the arachidonate-regulated Ca2+ (ARC) channel, a store-independent Orai channel, by plasma membrane STIM1. J Physiol. 2013;591:3507-23 pubmed publisher
Sanchez Collado J, Lopez J, Jardin I, Berna Erro A, Camello P, Cantonero C, et al. Orai1α, but not Orai1β, co-localizes with TRPC1 and is required for its plasma membrane location and activation in HeLa cells. Cell Mol Life Sci. 2022;79:33 pubmed publisher
Vallese F, Catoni C, Cieri D, Barazzuol L, Ramírez O, Calore V, et al. An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo. Nat Commun. 2020;11:6069 pubmed publisher
Shi J, Miralles F, Kinet J, Birnbaumer L, Large W, Albert A. Evidence that Orai1 does not contribute to store-operated TRPC1 channels in vascular smooth muscle cells. Channels (Austin). 2017;11:329-339 pubmed publisher
Lim H, Woon H, Han J, Baba Y, Kurosaki T, Lee M, et al. UDP-Induced Phagocytosis and ATP-Stimulated Chemotactic Migration Are Impaired in STIM1-/- Microglia In Vitro and In Vivo. Mediators Inflamm. 2017;2017:8158514 pubmed publisher
Shi J, Miralles F, Birnbaumer L, Large W, Albert A. Store-operated interactions between plasmalemmal STIM1 and TRPC1 proteins stimulate PLCβ1 to induce TRPC1 channel activation in vascular smooth muscle cells. J Physiol. 2017;595:1039-1058 pubmed publisher
Liu X, Berry C, Ruthel G, Madara J, MacGillivray K, Gray C, et al. T Cell Receptor-induced Nuclear Factor κB (NF-κB) Signaling and Transcriptional Activation Are Regulated by STIM1- and Orai1-mediated Calcium Entry. J Biol Chem. 2016;291:8440-52 pubmed publisher
Lefievre L, Nash K, Mansell S, Costello S, Punt E, Correia J, et al. 2-APB-potentiated channels amplify CatSper-induced Ca(2+) signals in human sperm. Biochem J. 2012;448:189-200 pubmed publisher
Yu P, Zhang Z, Hao B, Zhao Y, Zhang L, Lee H, et al. A novel fluorescent cell membrane-permeable caged cyclic ADP-ribose analogue. J Biol Chem. 2012;287:24774-83 pubmed publisher
Srikanth S, Jung H, Kim K, Souda P, Whitelegge J, Gwack Y. A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. Nat Cell Biol. 2010;12:436-46 pubmed publisher
product information
Cat.# :
610954
Brand :
BD Transduction Laboratories
Name :
GOK
Format :
Purified
Clone :
44/GOK
Isotype :
Ms IgG 2a
React :
Hu , Ms , Rat
Conc :
250 µg/ml
Apps :
IF , WB
Size :
50 µg
Status :
RUO
more info or order :
company information