product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
N-Cadherin
catalog :
610921
quantity :
150 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
32/N-Cadherin
reactivity :
African green monkey, human, mouse, rat, chicken, domestic sheep
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation, immunohistochemistry knockout validation
more info or order :
citations: 73
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; mouse; loading ...; fig 2a, 2b
  • western blot knockout validation; mouse; loading ...; fig 2c
Logan C, Rajakaruna S, Bowen C, Radice G, Robinson M, Menko A. N-cadherin regulates signaling mechanisms required for lens fiber cell elongation and lens morphogenesis. Dev Biol. 2017;428:118-134 pubmed publisher
  • western blot; human; fig 1d
Oh T, Lee M, Lee Y, Kim G, Lee D, You J, et al. PGC1α Loss Promotes Lung Cancer Metastasis through Epithelial-Mesenchymal Transition. Cancers (Basel). 2021;13: pubmed publisher
  • immunohistochemistry; mouse; 1:250; loading ...; fig s3-1e
Morcom L, Gobius I, Marsh A, Suárez R, Lim J, Bridges C, et al. DCC regulates astroglial development essential for telencephalic morphogenesis and corpus callosum formation. elife. 2021;10: pubmed publisher
  • western blot; human; 1:500; loading ...; fig 5a
Fayad R, Rojas M, Partisani M, Finetti P, Dib S, Abélanet S, et al. EFA6B regulates a stop signal for collective invasion in breast cancer. Nat Commun. 2021;12:2198 pubmed publisher
  • western blot; human; 1:5000; loading ...; fig 5f
Dilshat R, Fock V, Kenny C, Gerritsen I, Lasseur R, Travnickova J, et al. MITF reprograms the extracellular matrix and focal adhesion in melanoma. elife. 2021;10: pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig 5s1a
Dos Santos Carvalho S, Moreau M, Hien Y, Garcia M, Aubailly N, Henderson D, et al. Vangl2 acts at the interface between actin and N-cadherin to modulate mammalian neuronal outgrowth. elife. 2020;9: pubmed publisher
  • immunocytochemistry; human; 1:250; loading ...; fig 4e
Hiepen C, Jatzlau J, Hildebrandt S, Kampfrath B, Goktas M, Murgai A, et al. BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFβ responses and altered cell mechanics. PLoS Biol. 2019;17:e3000557 pubmed publisher
  • immunohistochemistry; mouse; 1:150; loading ...; fig 4e
Roy A, Murphy R, Deng M, MacDonald J, Bammler T, Aldinger K, et al. PI3K-Yap activity drives cortical gyrification and hydrocephalus in mice. elife. 2019;8: pubmed publisher
  • immunoprecipitation; human; 1:1000; loading ...; fig 7a
Kim E, Lisby A, Ma C, Lo N, Ehmer U, Hayer K, et al. Promotion of growth factor signaling as a critical function of β-catenin during HCC progression. Nat Commun. 2019;10:1909 pubmed publisher
  • western blot; human; 1:5000; loading ...; fig 1a
Hsu J, Xia W, Hsu Y, Chan L, Yu W, Cha J, et al. STT3-dependent PD-L1 accumulation on cancer stem cells promotes immune evasion. Nat Commun. 2018;9:1908 pubmed publisher
  • western blot; human; loading ...; fig 4e
Glaeser K, Urban M, Fenech E, Voloshanenko O, Kranz D, Lari F, et al. ERAD-dependent control of the Wnt secretory factor Evi. EMBO J. 2018;37: pubmed publisher
  • immunocytochemistry; human; 1:25; fig 1
  • immunohistochemistry; human; 1:25; fig 7
  • western blot; human; 1:1000; fig 1
Lee E, Wang J, Yumoto K, Jung Y, Cackowski F, Decker A, et al. DNMT1 Regulates Epithelial-Mesenchymal Transition and Cancer Stem Cells, Which Promotes Prostate Cancer Metastasis. Neoplasia. 2016;18:553-66 pubmed publisher
  • immunocytochemistry; human; fig 2a
Pailler E, Oulhen M, Billiot F, Galland A, Auger N, Faugeroux V, et al. Method for semi-automated microscopy of filtration-enriched circulating tumor cells. BMC Cancer. 2016;16:477 pubmed publisher
  • western blot; human; fig 4
Qi Y, Yu J, Han W, Fan X, Qian H, Wei H, et al. A splicing isoform of TEAD4 attenuates the Hippo-YAP signalling to inhibit tumour proliferation. Nat Commun. 2016;7:ncomms11840 pubmed publisher
  • western blot; human; fig 7
  • immunocytochemistry; African green monkey; 1:200; fig 1
  • western blot; African green monkey; fig 6
Wen M, Wang J, Chiu Y, Wang M, Lee S, Tai C. N-Cadherin Regulates Cell Migration Through a Rab5-Dependent Temporal Control of Macropinocytosis. Traffic. 2016;17:769-85 pubmed publisher
  • western blot; human; 1:2000; fig 4
Chang T, Chen C, Wu Y, Liu J, Kuo Y, Lee K, et al. Inflammation Promotes Expression of Stemness-Related Properties in HBV-Related Hepatocellular Carcinoma. PLoS ONE. 2016;11:e0149897 pubmed publisher
  • immunohistochemistry; mouse; 1:500; fig 5
  • western blot; mouse; fig 4a
Lee S, Shatadal S, Griep A. Dlg-1 Interacts With and Regulates the Activities of Fibroblast Growth Factor Receptors and EphA2 in the Mouse Lens. Invest Ophthalmol Vis Sci. 2016;57:707-18 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:200; fig 3
Skardal A, Devarasetty M, Forsythe S, Atala A, Soker S. A reductionist metastasis-on-a-chip platform for in vitro tumor progression modeling and drug screening. Biotechnol Bioeng. 2016;113:2020-32 pubmed publisher
  • western blot; human; 1:2000; fig 2f
Wang X, Jung Y, Jun S, Lee S, Wang W, Schneider A, et al. PAF-Wnt signaling-induced cell plasticity is required for maintenance of breast cancer cell stemness. Nat Commun. 2016;7:10633 pubmed publisher
  • western blot; human; fig 2
Wang J, Lu R, Yang J, Li H, He Z, Jing N, et al. TRPC6 specifically interacts with APP to inhibit its cleavage by γ-secretase and reduce Aβ production. Nat Commun. 2015;6:8876 pubmed publisher
  • western blot; rat; 1:500; fig 3
Brigidi G, Santyr B, Shimell J, Jovellar B, Bamji S. Activity-regulated trafficking of the palmitoyl-acyl transferase DHHC5. Nat Commun. 2015;6:8200 pubmed publisher
  • western blot; human; 1:500; fig 3
Zhang W, Gu Y, Sun Q, Siegel D, Tolias P, Yang Z, et al. Ex Vivo Maintenance of Primary Human Multiple Myeloma Cells through the Optimization of the Osteoblastic Niche. PLoS ONE. 2015;10:e0125995 pubmed publisher
  • immunocytochemistry; human; 1:200
  • immunohistochemistry; human; 1:200
Skardal A, Devarasetty M, Rodman C, Atala A, Soker S. Liver-Tumor Hybrid Organoids for Modeling Tumor Growth and Drug Response In Vitro. Ann Biomed Eng. 2015;43:2361-73 pubmed publisher
  • western blot; mouse; 1:500-1:2000; fig s2
Tom Dieck S, Kochen L, Hanus C, Heumüller M, Bartnik I, Nassim Assir B, et al. Direct visualization of newly synthesized target proteins in situ. Nat Methods. 2015;12:411-4 pubmed publisher
  • western blot; mouse; 1:500
Jiang X, Ma T, Zhang Y, Zhang H, Yin S, Zheng W, et al. Specific deletion of Cdh2 in Sertoli cells leads to altered meiotic progression and subfertility of mice. Biol Reprod. 2015;92:79 pubmed publisher
  • western blot; mouse
Cai K, Wang Y, Smith E, Smedberg J, Yang D, Yang W, et al. Global deletion of Trp53 reverts ovarian tumor phenotype of the germ cell-deficient white spotting variant (Wv) mice. Neoplasia. 2015;17:89-100 pubmed publisher
  • western blot; mouse; 1:2000
Tao W, Moore R, Smith E, Xu X. Hormonal induction and roles of Disabled-2 in lactation and involution. PLoS ONE. 2014;9:e110737 pubmed publisher
  • immunocytochemistry; human; fig 1
  • western blot; human; fig 1
Cai X, Dai Z, Reeves R, Caballero Benítez A, Duran K, Delrow J, et al. Autonomous stimulation of cancer cell plasticity by the human NKG2D lymphocyte receptor coexpressed with its ligands on cancer cells. PLoS ONE. 2014;9:e108942 pubmed publisher
  • western blot; human
Ohkawa T, Satake S, Yokoi N, Miyazaki Y, Ohshita T, Sobue G, et al. Identification and characterization of GABA(A) receptor autoantibodies in autoimmune encephalitis. J Neurosci. 2014;34:8151-63 pubmed publisher
  • western blot; human
Jung S, Ohk J, Jeong D, Li C, Lee S, Duan J, et al. Distinct regulatory effect of the p34SEI-1 oncoprotein on cancer metastasis in HER2/neu-positive and -negative cells. Int J Oncol. 2014;45:189-96 pubmed publisher
  • immunohistochemistry; mouse; 1:400
  • western blot; mouse; 1:2000
Nikitczuk J, Patil S, Matikainen Ankney B, Scarpa J, Shapiro M, Benson D, et al. N-cadherin regulates molecular organization of excitatory and inhibitory synaptic circuits in adult hippocampus in vivo. Hippocampus. 2014;24:943-962 pubmed publisher
  • western blot; mouse
Toyoshima D, Mandai K, Maruo T, Supriyanto I, Togashi H, Inoue T, et al. Afadin regulates puncta adherentia junction formation and presynaptic differentiation in hippocampal neurons. PLoS ONE. 2014;9:e89763 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Peitsch W, Doerflinger Y, Fischer Colbrie R, Huck V, Bauer A, Utikal J, et al. Desmoglein 2 depletion leads to increased migration and upregulation of the chemoattractant secretoneurin in melanoma cells. PLoS ONE. 2014;9:e89491 pubmed publisher
  • immunohistochemistry - paraffin section; human
  • immunohistochemistry - frozen section; mouse
Sigurdsson V, Ingthorsson S, Hilmarsdottir B, Gustafsdottir S, Franzdóttir S, Arason A, et al. Expression and functional role of sprouty-2 in breast morphogenesis. PLoS ONE. 2013;8:e60798 pubmed publisher
  • immunocytochemistry; rat; 1:250
  • western blot; rat; 1:1000
Martiáñez T, Lamarca A, Casals N, Gella A. N-cadherin expression is regulated by UTP in schwannoma cells. Purinergic Signal. 2013;9:259-70 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:5000
Mortillo S, Elste A, Ge Y, Patil S, Hsiao K, Huntley G, et al. Compensatory redistribution of neuroligins and N-cadherin following deletion of synaptic ?1-integrin. J Comp Neurol. 2012;520:2041-52 pubmed publisher
  • immunohistochemistry - frozen section; domestic sheep
  • western blot; domestic sheep
Chkourko H, Guerrero Serna G, Lin X, Darwish N, Pohlmann J, Cook K, et al. Remodeling of mechanical junctions and of microtubule-associated proteins accompany cardiac connexin43 lateralization. Heart Rhythm. 2012;9:1133-1140.e6 pubmed publisher
  • western blot; mouse; fig 4e
Yang Y, Ahn Y, Gibbons D, Zang Y, Lin W, Thilaganathan N, et al. The Notch ligand Jagged2 promotes lung adenocarcinoma metastasis through a miR-200-dependent pathway in mice. J Clin Invest. 2011;121:1373-85 pubmed publisher
  • western blot; human; fig 5
Walenda T, Bork S, Horn P, Wein F, Saffrich R, Diehlmann A, et al. Co-culture with mesenchymal stromal cells increases proliferation and maintenance of haematopoietic progenitor cells. J Cell Mol Med. 2010;14:337-50 pubmed publisher
Xu S, Bala S, Bendeck M. Discoidin domain receptor 1 deficiency in vascular smooth muscle cells leads to mislocalisation of N-cadherin contacts. Biol Open. 2019;8: pubmed publisher
Zeitz M, Calhoun P, James C, Taetzsch T, George K, Robel S, et al. Dynamic UTR Usage Regulates Alternative Translation to Modulate Gap Junction Formation during Stress and Aging. Cell Rep. 2019;27:2737-2747.e5 pubmed publisher
Hocevar B. Loss of Disabled-2 Expression in Pancreatic Cancer Progression. Sci Rep. 2019;9:7532 pubmed publisher
Vezzoli E, Caron I, Talpo F, Besusso D, Conforti P, Battaglia E, et al. Inhibiting pathologically active ADAM10 rescues synaptic and cognitive decline in Huntington's disease. J Clin Invest. 2019;130:2390-2403 pubmed publisher
Savci Heijink C, Halfwerk H, Hooijer G, Koster J, Horlings H, Meijer S, et al. Epithelial-to-mesenchymal transition status of primary breast carcinomas and its correlation with metastatic behavior. Breast Cancer Res Treat. 2019;174:649-659 pubmed publisher
Pyun J, Kim H, Jeong M, Ahn B, Vuong T, Lee D, et al. Cardiac specific PRMT1 ablation causes heart failure through CaMKII dysregulation. Nat Commun. 2018;9:5107 pubmed publisher
Bydoun M, Sterea A, Weaver I, Bharadwaj A, Waisman D. A novel mechanism of plasminogen activation in epithelial and mesenchymal cells. Sci Rep. 2018;8:14091 pubmed publisher
Walker J, Bleaken B, Romisher A, Alnwibit A, Menko A. In wound repair vimentin mediates the transition of mesenchymal leader cells to a myofibroblast phenotype. Mol Biol Cell. 2018;29:1555-1570 pubmed publisher
Vite A, Zhang C, Yi R, Emms S, Radice G. α-Catenin-dependent cytoskeletal tension controls Yap activity in the heart. Development. 2018;145: pubmed publisher
Chockley P, Chen J, Chen G, Beer D, Standiford T, Keshamouni V. Epithelial-mesenchymal transition leads to NK cell-mediated metastasis-specific immunosurveillance in lung cancer. J Clin Invest. 2018;128:1384-1396 pubmed publisher
Clements M, Byrne E, Camarillo Guerrero L, Cattin A, Zakka L, Ashraf A, et al. The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. Neuron. 2017;96:98-114.e7 pubmed publisher
Wei Y, Kim T, Peng D, Duan D, Gibbons D, Yamauchi M, et al. Fibroblast-specific inhibition of TGF-?1 signaling attenuates lung and tumor fibrosis. J Clin Invest. 2017;127:3675-3688 pubmed publisher
Whelan K, Chandramouleeswaran P, Tanaka K, Natsuizaka M, Guha M, Srinivasan S, et al. Autophagy supports generation of cells with high CD44 expression via modulation of oxidative stress and Parkin-mediated mitochondrial clearance. Oncogene. 2017;36:4843-4858 pubmed publisher
Liu C, Yu T, Huang Y, Cui L, Hong W. ETS (E26 transformation-specific) up-regulation of the transcriptional co-activator TAZ promotes cell migration and metastasis in prostate cancer. J Biol Chem. 2017;292:9420-9430 pubmed publisher
Falk S, Bugeon S, Ninkovic J, Pilz G, Postiglione M, Cremer H, et al. Time-Specific Effects of Spindle Positioning on Embryonic Progenitor Pool Composition and Adult Neural Stem Cell Seeding. Neuron. 2017;93:777-791.e3 pubmed publisher
Foerster P, Daclin M, Asm S, Faucourt M, Boletta A, Genovesio A, et al. mTORC1 signaling and primary cilia are required for brain ventricle morphogenesis. Development. 2017;144:201-210 pubmed publisher
Park D, Shekhar A, Marra C, Lin X, Vasquez C, Solinas S, et al. Fhf2 gene deletion causes temperature-sensitive cardiac conduction failure. Nat Commun. 2016;7:12966 pubmed publisher
Jansen S, Poppinga W, de Jager W, Lezoualc h F, Cheng X, Wieland T, et al. Epac1 links prostaglandin E2 to ?-catenin-dependent transcription during epithelial-to-mesenchymal transition. Oncotarget. 2016;7:46354-46370 pubmed publisher
Valcourt U, Carthy J, Okita Y, Alcaraz L, Kato M, Thuault S, et al. Analysis of Epithelial-Mesenchymal Transition Induced by Transforming Growth Factor β. Methods Mol Biol. 2016;1344:147-81 pubmed publisher
Sahu S, Garding A, Tiwari N, Thakurela S, Toedling J, Gebhard S, et al. JNK-dependent gene regulatory circuitry governs mesenchymal fate. EMBO J. 2015;34:2162-81 pubmed publisher
Chen L, Gibbons D, Goswami S, Cortez M, Ahn Y, Byers L, et al. Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression. Nat Commun. 2014;5:5241 pubmed publisher
Ottone C, Krusche B, Whitby A, Clements M, Quadrato G, Pitulescu M, et al. Direct cell-cell contact with the vascular niche maintains quiescent neural stem cells. Nat Cell Biol. 2014;16:1045-56 pubmed publisher
Hua K, Wang M, Chen M, Wei L, Chen C, Ko C, et al. The H3K9 methyltransferase G9a is a marker of aggressive ovarian cancer that promotes peritoneal metastasis. Mol Cancer. 2014;13:189 pubmed publisher
Tan C, Gardiner B, Hirokawa Y, Smith D, Burgess A. Analysis of Wnt signaling ?-catenin spatial dynamics in HEK293T cells. BMC Syst Biol. 2014;8:44 pubmed publisher
Sher Y, Wang L, Chuang L, Tsai M, Kuo T, Huang C, et al. ADAM9 up-regulates N-cadherin via miR-218 suppression in lung adenocarcinoma cells. PLoS ONE. 2014;9:e94065 pubmed publisher
Lee S, Han S, Kim J, Chung K, Han K. Expression of E-cadherin in pig kidney. J Vet Sci. 2013;14:381-6 pubmed
Jung Y, Kim J, Shiozawa Y, Wang J, Mishra A, Joseph J, et al. Recruitment of mesenchymal stem cells into prostate tumours promotes metastasis. Nat Commun. 2013;4:1795 pubmed publisher
BRUSES J. Cell surface localization of ?3?4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility. PLoS ONE. 2013;8:e62435 pubmed publisher
Tiwari N, Meyer Schaller N, Arnold P, Antoniadis H, Pachkov M, van Nimwegen E, et al. Klf4 is a transcriptional regulator of genes critical for EMT, including Jnk1 (Mapk8). PLoS ONE. 2013;8:e57329 pubmed publisher
Vervoort S, Lourenço A, van Boxtel R, Coffer P. SOX4 mediates TGF-?-induced expression of mesenchymal markers during mammary cell epithelial to mesenchymal transition. PLoS ONE. 2013;8:e53238 pubmed publisher
Jin H, Hong S, Woo S, Lee J, Choe T, Kim E, et al. Silencing of Twist1 sensitizes NSCLC cells to cisplatin via AMPK-activated mTOR inhibition. Cell Death Dis. 2012;3:e319 pubmed publisher
Géraud C, Evdokimov K, Straub B, Peitsch W, Demory A, Dörflinger Y, et al. Unique cell type-specific junctional complexes in vascular endothelium of human and rat liver sinusoids. PLoS ONE. 2012;7:e34206 pubmed publisher
Kotb A, Hierholzer A, Kemler R. Replacement of E-cadherin by N-cadherin in the mammary gland leads to fibrocystic changes and tumor formation. Breast Cancer Res. 2011;13:R104 pubmed publisher
Martin J, Herbert B, Hocevar B. Disabled-2 downregulation promotes epithelial-to-mesenchymal transition. Br J Cancer. 2010;103:1716-23 pubmed publisher
product information
Cat.# :
610921
Brand :
BD Transduction Laboratories
Name :
N-Cadherin
Format :
Purified
Clone :
32/N-Cadherin
Isotype :
Ms IgG 1
React :
Hu , Ms , Rat , Chick
Conc :
250 µg/ml
Apps :
IF , WB
Size :
150 µg
Status :
RUO
more info or order :
company information