product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
p62 Lck Ligand
catalog :
610833
quantity :
150 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3/P62 LCK LIGAND
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, reverse phase protein lysate microarray
more info or order :
citations: 76
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:2000; loading ...; fig 4b
Prins M, Giugliano F, van Roest M, van de Graaf S, Koelink P, Wildenberg M. Thiopurines correct the effects of autophagy impairment on intestinal healing - a potential role for ARHGAP18/RhoA. Dis Model Mech. 2021;14: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 4a
Cendrowski J, Kaczmarek M, Mazur M, Kuzmicz Kowalska K, Jastrzębski K, Brewińska Olchowik M, et al. Splicing variation of BMP2K balances abundance of COPII assemblies and autophagic degradation in erythroid cells. elife. 2020;9: pubmed publisher
  • western blot; human; loading ...; fig 3c
Chen W, Wang Q, Xu X, Saxton B, Tessema M, Leng S, et al. Vasorin/ATIA Promotes Cigarette Smoke-Induced Transformation of Human Bronchial Epithelial Cells by Suppressing Autophagy-Mediated Apoptosis. Transl Oncol. 2020;13:32-41 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1d, 1f
  • western blot; human; 1:1000; loading ...; fig 8b
Yang Y, Willis T, Button R, Strang C, Fu Y, Wen X, et al. Cytoplasmic DAXX drives SQSTM1/p62 phase condensation to activate Nrf2-mediated stress response. Nat Commun. 2019;10:3759 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 6e
  • western blot; human; 1:2500; loading ...; fig 1e
Goodwin J, Dowdle W, Dejesus R, Wang Z, Bergman P, Kobylarz M, et al. Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9. Cell Rep. 2017;20:2341-2356 pubmed publisher
  • immunocytochemistry; human; 1:2000; fig 7
  • western blot; human; 1:2000; fig 3b
Wang G, Zhou H, Strulovici Barel Y, Al Hijji M, Ou X, Salit J, et al. Role of OSGIN1 in mediating smoking-induced autophagy in the human airway epithelium. Autophagy. 2017;13:1205-1220 pubmed publisher
  • western blot; human; loading ...; fig e1c
  • western blot; mouse; loading ...; fig e2d
Ashkenazi A, Bento C, Ricketts T, Vicinanza M, Siddiqi F, Pavel M, et al. Polyglutamine tracts regulate beclin 1-dependent autophagy. Nature. 2017;545:108-111 pubmed publisher
  • reverse phase protein lysate microarray; human; loading ...; fig st6
Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
  • reverse phase protein lysate microarray; human; loading ...; fig 3a
. Integrated genomic and molecular characterization of cervical cancer. Nature. 2017;543:378-384 pubmed publisher
  • western blot; human; loading ...; fig 2a
Zhang L, Dai F, Cui L, Zhou B, Guo Y. Up-regulation of the active form of small GTPase Rab13 promotes macroautophagy in vascular endothelial cells. Biochim Biophys Acta Mol Cell Res. 2017;1864:613-624 pubmed publisher
  • western blot; human; loading ...; fig 3a
Van Puyenbroeck V, Claeys E, Schols D, Bell T, Vermeire K. A Proteomic Survey Indicates Sortilin as a Secondary Substrate of the ER Translocation Inhibitor Cyclotriazadisulfonamide (CADA). Mol Cell Proteomics. 2017;16:157-167 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 3d
Fernández B, Fdez E, Gomez Suaga P, Gil F, Molina Villalba I, Ferrer I, et al. Iron overload causes endolysosomal deficits modulated by NAADP-regulated 2-pore channels and RAB7A. Autophagy. 2016;12:1487-506 pubmed publisher
  • western blot; human
Dejesus R, Moretti F, McAllister G, Wang Z, Bergman P, Liu S, et al. Functional CRISPR screening identifies the ufmylation pathway as a regulator of SQSTM1/p62. elife. 2016;5: pubmed publisher
  • western blot; human; fig s1
Karvela M, Baquero P, Kuntz E, Mukhopadhyay A, Mitchell R, Allan E, et al. ATG7 regulates energy metabolism, differentiation and survival of Philadelphia-chromosome-positive cells. Autophagy. 2016;12:936-48 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; tbl 1
Ito M, Nakamura K, Mori F, Miki Y, Tanji K, Wakabayashi K. Novel eosinophilic neuronal cytoplasmic inclusions in the external cuneate nucleus of humans. Neuropathology. 2016;36:441-447 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 2
  • western blot; human; 1:1000; fig 2
Button R, Vincent J, Strang C, Luo S. Dual PI-3 kinase/mTOR inhibition impairs autophagy flux and induces cell death independent of apoptosis and necroptosis. Oncotarget. 2016;7:5157-75 pubmed publisher
  • immunocytochemistry; human; fig 4
Puvirajesinghe T, Bertucci F, Jain A, Scerbo P, Belotti E, Audebert S, et al. Identification of p62/SQSTM1 as a component of non-canonical Wnt VANGL2-JNK signalling in breast cancer. Nat Commun. 2016;7:10318 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1a
  • western blot; human; loading ...; fig 3d
Ivankovic D, Chau K, Schapira A, Gegg M. Mitochondrial and lysosomal biogenesis are activated following PINK1/parkin-mediated mitophagy. J Neurochem. 2016;136:388-402 pubmed publisher
  • immunohistochemistry; rat; fig 6
  • western blot; rat; fig 5
Chen D, Tao X, Wang Y, Tian F, Wei Y, Chen G, et al. Curcumin accelerates reendothelialization and ameliorates intimal hyperplasia in balloon-injured rat carotid artery via the upregulation of endothelial cell autophagy. Int J Mol Med. 2015;36:1563-71 pubmed publisher
  • western blot; human; 1:1000; fig 2
Bae E, Yang N, Lee C, Kim S, Lee H, Lee S. Haploinsufficiency of cathepsin D leads to lysosomal dysfunction and promotes cell-to-cell transmission of α-synuclein aggregates. Cell Death Dis. 2015;6:e1901 pubmed publisher
  • western blot; human; fig 2e
Levin A, Koelink P, Bloemendaal F, Vos A, D Haens G, van den Brink G, et al. Autophagy Contributes to the Induction of Anti-TNF Induced Macrophages. J Crohns Colitis. 2016;10:323-9 pubmed publisher
  • western blot; human; 1:1000; fig 4
Cañeque T, Gomes F, Mai T, Maestri G, Malacria M, Rodriguez R. Synthesis of marmycin A and investigation into its cellular activity. Nat Chem. 2015;7:744-51 pubmed publisher
  • western blot; human; loading ...; fig 2c
Colecchia D, Rossi M, Sasdelli F, Sanzone S, Strambi A, Chiariello M. MAPK15 mediates BCR-ABL1-induced autophagy and regulates oncogene-dependent cell proliferation and tumor formation. Autophagy. 2015;11:1790-802 pubmed publisher
  • western blot; human; fig 4
Zhang L, Dai F, Sheng P, Chen Z, Xu Q, Guo Y. Resveratrol analogue 3,4,4'-trihydroxy-trans-stilbene induces apoptosis and autophagy in human non-small-cell lung cancer cells in vitro. Acta Pharmacol Sin. 2015;36:1256-65 pubmed publisher
  • immunocytochemistry; human; 1:200
Schraivogel D, Schindler S, Danner J, Kremmer E, Pfaff J, Hannus S, et al. Importin-β facilitates nuclear import of human GW proteins and balances cytoplasmic gene silencing protein levels. Nucleic Acids Res. 2015;43:7447-61 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 5
Smith B, Vance C, Scotter E, Troakes C, Wong C, Topp S, et al. Novel mutations support a role for Profilin 1 in the pathogenesis of ALS. Neurobiol Aging. 2015;36:1602.e17-27 pubmed publisher
  • western blot; human
Zhang L, Dai F, Cui L, Jing H, Fan P, Tan X, et al. Novel role for TRPC4 in regulation of macroautophagy by a small molecule in vascular endothelial cells. Biochim Biophys Acta. 2015;1853:377-87 pubmed publisher
  • western blot; mouse; 1:1000
Guo H, Chitiprolu M, Gagnon D, Meng L, Perez Iratxeta C, Lagace D, et al. Autophagy supports genomic stability by degrading retrotransposon RNA. Nat Commun. 2014;5:5276 pubmed publisher
  • western blot; human; 1:5000; fig 4
Dowdle W, Nyfeler B, Nagel J, Elling R, Liu S, Triantafellow E, et al. Selective VPS34 inhibitor blocks autophagy and uncovers a role for NCOA4 in ferritin degradation and iron homeostasis in vivo. Nat Cell Biol. 2014;16:1069-79 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 2
  • immunocytochemistry; mouse; 1:100; fig s5
Zhang Y, Jansen West K, Xu Y, Gendron T, Bieniek K, Lin W, et al. Aggregation-prone c9FTD/ALS poly(GA) RAN-translated proteins cause neurotoxicity by inducing ER stress. Acta Neuropathol. 2014;128:505-24 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100
Mori F, Watanabe Y, Miki Y, Tanji K, Odagiri S, Eto K, et al. Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases. Neuropathology. 2014;34:140-7 pubmed
  • immunohistochemistry - paraffin section; human; 1:100; fig 4
Bodi I, Curran O, Selway R, Elwes R, Burrone J, Laxton R, et al. Two cases of multinodular and vacuolating neuronal tumour. Acta Neuropathol Commun. 2014;2:7 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Manzoni C, Mamais A, Dihanich S, McGoldrick P, Devine M, Zerle J, et al. Pathogenic Parkinson's disease mutations across the functional domains of LRRK2 alter the autophagic/lysosomal response to starvation. Biochem Biophys Res Commun. 2013;441:862-6 pubmed publisher
  • western blot; human; fig 1a
Newman A, Scholefield C, Kemp A, Newman M, McIver E, Kamal A, et al. TBK1 kinase addiction in lung cancer cells is mediated via autophagy of Tax1bp1/Ndp52 and non-canonical NF-κB signalling. PLoS ONE. 2012;7:e50672 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100
Kon T, Mori F, Tanji K, Miki Y, Kimura T, Wakabayashi K. Giant cell polymyositis and myocarditis associated with myasthenia gravis and thymoma. Neuropathology. 2013;33:281-7 pubmed publisher
Gleixner A, Verdone B, Otte C, Anderson E, Ramesh N, Shapiro O, et al. NUP62 localizes to ALS/FTLD pathological assemblies and contributes to TDP-43 insolubility. Nat Commun. 2022;13:3380 pubmed publisher
Jia R, Bonifacino J. Negative regulation of autophagy by UBA6-BIRC6-mediated ubiquitination of LC3. elife. 2019;8: pubmed publisher
Granato M, Gilardini Montani M, Zompetta C, Santarelli R, Gonnella R, Romeo M, et al. Quercetin Interrupts the Positive Feedback Loop Between STAT3 and IL-6, Promotes Autophagy, and Reduces ROS, Preventing EBV-Driven B Cell Immortalization. Biomolecules. 2019;9: pubmed publisher
Nair S, Strohecker A, Persaud A, Bissa B, Muruganandan S, McElroy C, et al. Adult stem cell deficits drive Slc29a3 disorders in mice. Nat Commun. 2019;10:2943 pubmed publisher
Chiramel A, Meyerson N, McNally K, Broeckel R, Montoya V, Méndez Solís O, et al. TRIM5α Restricts Flavivirus Replication by Targeting the Viral Protease for Proteasomal Degradation. Cell Rep. 2019;27:3269-3283.e6 pubmed publisher
Tian Y, Wang J, Huang W, Zeng S, Jiao B, Liu Z, et al. Expansion of Human-Specific GGC Repeat in Neuronal Intranuclear Inclusion Disease-Related Disorders. Am J Hum Genet. 2019;: pubmed publisher
Mamelona J, Filice L, Oussedik Y, Crapoulet N, Ouellette R, Marrero A. A novel missense mutation in the MYH7 gene causes an uncharacteristic phenotype of myosin storage myopathy: a case report. BMC Med Genet. 2019;20:78 pubmed publisher
Shaw B, Burrell C, Green D, Navarro Martinez A, Scott D, Daroszewska A, et al. Molecular insights into an ancient form of Paget's disease of bone. Proc Natl Acad Sci U S A. 2019;116:10463-10472 pubmed publisher
Valverde D, Yu S, Boggavarapu V, Kumar N, Lees J, Walz T, et al. ATG2 transports lipids to promote autophagosome biogenesis. J Cell Biol. 2019;218:1787-1798 pubmed publisher
Wang Y, Xiong H, Liu D, Hill C, Ertay A, Li J, et al. Autophagy inhibition specifically promotes epithelial-mesenchymal transition and invasion in RAS-mutated cancer cells. Autophagy. 2019;15:886-899 pubmed publisher
Birgisdottir A, Mouilleron S, Bhujabal Z, Wirth M, Sjøttem E, Evjen G, et al. Members of the autophagy class III phosphatidylinositol 3-kinase complex I interact with GABARAP and GABARAPL1 via LIR motifs. Autophagy. 2019;15:1333-1355 pubmed publisher
Larson C, Sandoz K, Cockrell D, Heinzen R. Noncanonical Inhibition of mTORC1 by Coxiella burnetii Promotes Replication within a Phagolysosome-Like Vacuole. MBio. 2019;10: pubmed publisher
Hoffmann S, Orlando M, Andrzejak E, Bruns C, Trimbuch T, Rosenmund C, et al. Light-Activated ROS Production Induces Synaptic Autophagy. J Neurosci. 2019;39:2163-2183 pubmed publisher
Leonardi M, Perna E, Tronnolone S, Colecchia D, Chiariello M. Activated kinase screening identifies the IKBKE oncogene as a positive regulator of autophagy. Autophagy. 2018;:1-15 pubmed publisher
Pellegrini P, Serviss J, Lundbäck T, Bancaro N, Mazurkiewicz M, Kolosenko I, et al. A drug screening assay on cancer cells chronically adapted to acidosis. Cancer Cell Int. 2018;18:147 pubmed publisher
Colecchia D, Dapporto F, Tronnolone S, Salvini L, Chiariello M. MAPK15 is part of the ULK complex and controls its activity to regulate early phases of the autophagic process. J Biol Chem. 2018;293:15962-15976 pubmed publisher
Mamais A, Manzoni C, Nazish I, Arber C, Sonustun B, Wray S, et al. Analysis of macroautophagy related proteins in G2019S LRRK2 Parkinson's disease brains with Lewy body pathology. Brain Res. 2018;1701:75-84 pubmed publisher
Meyer N, Zielke S, Michaelis J, Linder B, Warnsmann V, Rakel S, et al. AT 101 induces early mitochondrial dysfunction and HMOX1 (heme oxygenase 1) to trigger mitophagic cell death in glioma cells. Autophagy. 2018;14:1693-1709 pubmed publisher
Marcassa E, Kallinos A, Jardine J, Rusilowicz Jones E, Martinez A, Kuehl S, et al. Dual role of USP30 in controlling basal pexophagy and mitophagy. EMBO Rep. 2018;19: pubmed publisher
Manzoni C, Mamais A, Dihanich S, Soutar M, Plun Favreau H, Bandopadhyay R, et al. mTOR independent alteration in ULK1 Ser758 phosphorylation following chronic LRRK2 kinase inhibition. Biosci Rep. 2018;38: pubmed publisher
Søreng K, Munson M, Lamb C, Bjørndal G, Pankiv S, Carlsson S, et al. SNX18 regulates ATG9A trafficking from recycling endosomes by recruiting Dynamin-2. EMBO Rep. 2018;19: pubmed publisher
Colecchia D, Stasi M, Leonardi M, Manganelli F, Nolano M, Veneziani B, et al. Alterations of autophagy in the peripheral neuropathy Charcot-Marie-Tooth type 2B. Autophagy. 2017;: pubmed publisher
Linares J, Cordes T, Duran A, Reina Campos M, Valencia T, Ahn C, et al. ATF4-Induced Metabolic Reprograming Is a Synthetic Vulnerability of the p62-Deficient Tumor Stroma. Cell Metab. 2017;26:817-829.e6 pubmed publisher
Goode A, Butler K, Long J, Cavey J, Scott D, Shaw B, et al. Defective recognition of LC3B by mutant SQSTM1/p62 implicates impairment of autophagy as a pathogenic mechanism in ALS-FTLD. Autophagy. 2016;12:1094-104 pubmed publisher
Casellas A, Mallol C, Salavert A, Jimenez V, Garcia M, Agudo J, et al. Insulin-like Growth Factor 2 Overexpression Induces β-Cell Dysfunction and Increases Beta-cell Susceptibility to Damage. J Biol Chem. 2015;290:16772-85 pubmed publisher
Ma L, Maruwge W, Strambi A, D Arcy P, Pellegrini P, Kis L, et al. SIRT1 and SIRT2 inhibition impairs pediatric soft tissue sarcoma growth. Cell Death Dis. 2014;5:e1483 pubmed publisher
Moreau K, Fleming A, Imarisio S, Lopez Ramirez A, Mercer J, Jimenez Sanchez M, et al. PICALM modulates autophagy activity and tau accumulation. Nat Commun. 2014;5:4998 pubmed publisher
Peng N, Meng N, Wang S, Zhao F, Zhao J, Su L, et al. An activator of mTOR inhibits oxLDL-induced autophagy and apoptosis in vascular endothelial cells and restricts atherosclerosis in apolipoprotein E?/? mice. Sci Rep. 2014;4:5519 pubmed publisher
Nicot A, Lo Verso F, Ratti F, Pilot Storck F, Streichenberger N, Sandri M, et al. Phosphorylation of NBR1 by GSK3 modulates protein aggregation. Autophagy. 2014;10:1036-53 pubmed publisher
Santarelli R, Gonnella R, Di Giovenale G, Cuomo L, Capobianchi A, Granato M, et al. STAT3 activation by KSHV correlates with IL-10, IL-6 and IL-23 release and an autophagic block in dendritic cells. Sci Rep. 2014;4:4241 pubmed publisher
Zhang X, Fryknäs M, Hernlund E, Fayad W, De Milito A, Olofsson M, et al. Induction of mitochondrial dysfunction as a strategy for targeting tumour cells in metabolically compromised microenvironments. Nat Commun. 2014;5:3295 pubmed publisher
Scotter E, Vance C, Nishimura A, Lee Y, Chen H, Urwin H, et al. Differential roles of the ubiquitin proteasome system and autophagy in the clearance of soluble and aggregated TDP-43 species. J Cell Sci. 2014;127:1263-78 pubmed publisher
Li H, Huang S, Wang S, Zhao J, Su L, Zhao B, et al. Targeting annexin A7 by a small molecule suppressed the activity of phosphatidylcholine-specific phospholipase C in vascular endothelial cells and inhibited atherosclerosis in apolipoprotein E?/?mice. Cell Death Dis. 2013;4:e806 pubmed publisher
Burchell V, Nelson D, Sanchez Martinez A, Delgado Camprubi M, Ivatt R, Pogson J, et al. The Parkinson's disease-linked proteins Fbxo7 and Parkin interact to mediate mitophagy. Nat Neurosci. 2013;16:1257-65 pubmed publisher
Manzoni C, Mamais A, Dihanich S, Abeti R, Soutar M, Plun Favreau H, et al. Inhibition of LRRK2 kinase activity stimulates macroautophagy. Biochim Biophys Acta. 2013;1833:2900-2910 pubmed publisher
Granato M, Lacconi V, Peddis M, Lotti L, di Renzo L, Renzo L, et al. HSP70 inhibition by 2-phenylethynesulfonamide induces lysosomal cathepsin D release and immunogenic cell death in primary effusion lymphoma. Cell Death Dis. 2013;4:e730 pubmed publisher
Lorin S, Tol M, Bauvy C, Strijland A, Poüs C, Verhoeven A, et al. Glutamate dehydrogenase contributes to leucine sensing in the regulation of autophagy. Autophagy. 2013;9:850-60 pubmed publisher
Yue W, Hamai A, Tonelli G, Bauvy C, Nicolas V, Tharinger H, et al. Inhibition of the autophagic flux by salinomycin in breast cancer stem-like/progenitor cells interferes with their maintenance. Autophagy. 2013;9:714-29 pubmed publisher
Chargui A, Cesaro A, Mimouna S, Fareh M, Brest P, Naquet P, et al. Subversion of autophagy in adherent invasive Escherichia coli-infected neutrophils induces inflammation and cell death. PLoS ONE. 2012;7:e51727 pubmed publisher
Colecchia D, Strambi A, Sanzone S, Iavarone C, Rossi M, Dall Armi C, et al. MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins. Autophagy. 2012;8:1724-40 pubmed publisher
Bensaad K, Cheung E, Vousden K. Modulation of intracellular ROS levels by TIGAR controls autophagy. EMBO J. 2009;28:3015-26 pubmed publisher
product information
Cat.# :
610833
Brand :
BD Transduction Laboratories
Name :
p62 Lck Ligand
Format :
Purified
Clone :
3/P62 LCK LIGAND
Isotype :
Ms IgG 1
React :
Hu , Ms , Rat
Conc :
250 µg/ml
Apps :
IF , WB
Size :
150 µg
Status :
RUO
more info or order :
company information