product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
p62 Lck Ligand
catalog :
610832
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3/P62 LCK LIGAND
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation
more info or order :
citations: 82
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; loading ...; fig 4e
Poon A, Saini H, Sethi S, O Sullivan G, Plun Favreau H, Wray S, et al. The role of SQSTM1 (p62) in mitochondrial function and clearance in human cortical neurons. Stem Cell Reports. 2021;16:1276-1289 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig s5i
Jena K, Kolapalli S, Mehto S, Nath P, Das B, Sahoo P, et al. TRIM16 controls assembly and degradation of protein aggregates by modulating the p62-NRF2 axis and autophagy. EMBO J. 2018;37: pubmed publisher
  • western blot; human; 1:10,000; loading ...; fig 4e
Law K, Bronte Tinkew D, Di Pietro E, Snowden A, Jones R, Moser A, et al. The peroxisomal AAA ATPase complex prevents pexophagy and development of peroxisome biogenesis disorders. Autophagy. 2017;13:868-884 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 5f
Jeong J, Kim W, Kim L, VanHouten J, Wysolmerski J. HER2 signaling regulates HER2 localization and membrane retention. PLoS ONE. 2017;12:e0174849 pubmed publisher
  • western blot; human; loading ...; fig 1c
Marcassa E, Raimondi M, Anwar T, Eskelinen E, Myers M, Triolo G, et al. Calpain mobilizes Atg9/Bif-1 vesicles from Golgi stacks upon autophagy induction by thapsigargin. Biol Open. 2017;6:551-562 pubmed publisher
  • immunocytochemistry; human; loading ...
Jeong J, VanHouten J, Kim W, Dann P, Sullivan C, Choi J, et al. The scaffolding protein NHERF1 regulates the stability and activity of the tyrosine kinase HER2. J Biol Chem. 2017;292:6555-6568 pubmed publisher
  • western blot; human; loading ...; fig 3a
Van Puyenbroeck V, Claeys E, Schols D, Bell T, Vermeire K. A Proteomic Survey Indicates Sortilin as a Secondary Substrate of the ER Translocation Inhibitor Cyclotriazadisulfonamide (CADA). Mol Cell Proteomics. 2017;16:157-167 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1f
Fracchiolla D, Sawa Makarska J, Zens B, Ruiter A, Zaffagnini G, Brezovich A, et al. Mechanism of cargo-directed Atg8 conjugation during selective autophagy. elife. 2016;5: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:250; loading ...; fig 6b
Yang C, Danielson E, Qiao T, Metterville J, Brown R, Landers J, et al. Mutant PFN1 causes ALS phenotypes and progressive motor neuron degeneration in mice by a gain of toxicity. Proc Natl Acad Sci U S A. 2016;113:E6209-E6218 pubmed
  • immunohistochemistry - frozen section; mouse; 1:500; fig s2
Alves S, Marais T, Biferi M, Furling D, Marinello M, El Hachimi K, et al. Lentiviral vector-mediated overexpression of mutant ataxin-7 recapitulates SCA7 pathology and promotes accumulation of the FUS/TLS and MBNL1 RNA-binding proteins. Mol Neurodegener. 2016;11:58 pubmed publisher
  • immunocytochemistry; mouse; fig 3
Hamlin A, Basford J, Jaeschke A, Hui D. LRP1 Protein Deficiency Exacerbates Palmitate-induced Steatosis and Toxicity in Hepatocytes. J Biol Chem. 2016;291:16610-9 pubmed publisher
  • western blot; human; 1:1000; fig 1
Boada Romero E, Serramito Gómez I, Sacristán M, Boone D, Xavier R, Pimentel Muiños F. The T300A Crohn's disease risk polymorphism impairs function of the WD40 domain of ATG16L1. Nat Commun. 2016;7:11821 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:500; fig 5
  • western blot; human; fig 1
Jennewein L, Ronellenfitsch M, Antonietti P, Ilina E, Jung J, Stadel D, et al. Diagnostic and clinical relevance of the autophago-lysosomal network in human gliomas. Oncotarget. 2016;7:20016-32 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; tbl 1
Ito M, Nakamura K, Mori F, Miki Y, Tanji K, Wakabayashi K. Novel eosinophilic neuronal cytoplasmic inclusions in the external cuneate nucleus of humans. Neuropathology. 2016;36:441-447 pubmed publisher
  • western blot; human; 1:2000; fig 4
Kim N, Kim M, Sung P, Bae Y, Shin E, Yoo J. Interferon-inducible protein SCOTIN interferes with HCV replication through the autolysosomal degradation of NS5A. Nat Commun. 2016;7:10631 pubmed publisher
  • western blot; human; 1:2000; fig 1
Tokuda E, Brännström T, Andersen P, Marklund S. Low autophagy capacity implicated in motor system vulnerability to mutant superoxide dismutase. Acta Neuropathol Commun. 2016;4:6 pubmed publisher
  • western blot; mouse; 1:1000; fig 4
Ruiz A, Rockfield S, Taran N, Haller E, Engelman R, Flores I, et al. Effect of hydroxychloroquine and characterization of autophagy in a mouse model of endometriosis. Cell Death Dis. 2016;7:e2059 pubmed publisher
  • immunocytochemistry; human; fig 5
Jeong J, VanHouten J, Dann P, Kim W, Sullivan C, Yu H, et al. PMCA2 regulates HER2 protein kinase localization and signaling and promotes HER2-mediated breast cancer. Proc Natl Acad Sci U S A. 2016;113:E282-90 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 6fs1b
  • western blot; human; loading ...; fig 6c
Wurzer B, Zaffagnini G, Fracchiolla D, Turco E, Abert C, Romanov J, et al. Oligomerization of p62 allows for selection of ubiquitinated cargo and isolation membrane during selective autophagy. elife. 2015;4:e08941 pubmed publisher
  • western blot; human; 1:1000; fig 4b
Granato M, Gilardini Montani M, Filardi M, Faggioni A, Cirone M. Capsaicin triggers immunogenic PEL cell death, stimulates DCs and reverts PEL-induced immune suppression. Oncotarget. 2015;6:29543-54 pubmed publisher
  • western blot; human; 1:1000; fig 7
Sakabe I, Hu R, Jin L, Clarke R, Kasid U. TMEM33: a new stress-inducible endoplasmic reticulum transmembrane protein and modulator of the unfolded protein response signaling. Breast Cancer Res Treat. 2015;153:285-97 pubmed publisher
  • immunocytochemistry; human; fig 4
  • western blot; human; fig 4
Riz I, Hawley T, Hawley R. KLF4-SQSTM1/p62-associated prosurvival autophagy contributes to carfilzomib resistance in multiple myeloma models. Oncotarget. 2015;6:14814-31 pubmed
  • immunocytochemistry; mouse
Cemma M, Lam G, Stöckli M, Higgins D, Brumell J. Strain-Specific Interactions of Listeria monocytogenes with the Autophagy System in Host Cells. PLoS ONE. 2015;10:e0125856 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig s3
Freischmidt A, Wieland T, Richter B, Ruf W, Schaeffer V, Müller K, et al. Haploinsufficiency of TBK1 causes familial ALS and fronto-temporal dementia. Nat Neurosci. 2015;18:631-6 pubmed publisher
  • western blot; human
Shi S, Wang Q, Xu J, Jang J, Padilla M, Nyunoya T, et al. Synergistic anticancer effect of cisplatin and Chal-24 combination through IAP and c-FLIPL degradation, Ripoptosome formation and autophagy-mediated apoptosis. Oncotarget. 2015;6:1640-51 pubmed
  • immunocytochemistry; human; fig 6
Guerrero A, Iglesias C, Raguz S, Floridia E, Gil J, Pombo C, et al. The cerebral cavernous malformation 3 gene is necessary for senescence induction. Aging Cell. 2015;14:274-83 pubmed publisher
  • western blot; human; fig 6
Mudie S, Bandarra D, Batie M, Biddlestone J, Moniz S, Ortmann B, et al. PITX1, a specificity determinant in the HIF-1α-mediated transcriptional response to hypoxia. Cell Cycle. 2014;13:3878-91 pubmed publisher
  • immunocytochemistry; human; fig 3
  • western blot; human; fig 1
Brandstaetter H, Kishi Itakura C, Tumbarello D, Manstein D, Buss F. Loss of functional MYO1C/myosin 1c, a motor protein involved in lipid raft trafficking, disrupts autophagosome-lysosome fusion. Autophagy. 2014;10:2310-23 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Sarkar C, Zhao Z, Aungst S, Sabirzhanov B, Faden A, Lipinski M. Impaired autophagy flux is associated with neuronal cell death after traumatic brain injury. Autophagy. 2014;10:2208-22 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:500
Nakamura M, Murray M, Lin W, Kusaka H, Dickson D. Optineurin immunoreactivity in neuronal and glial intranuclear inclusions in adult-onset neuronal intranuclear inclusion disease. Am J Neurodegener Dis. 2014;3:93-102 pubmed
  • western blot; human; fig 3
Elzi D, Song M, Hakala K, Weintraub S, Shiio Y. Proteomic Analysis of the EWS-Fli-1 Interactome Reveals the Role of the Lysosome in EWS-Fli-1 Turnover. J Proteome Res. 2014;13:3783-91 pubmed publisher
  • western blot; human; 1:500
Fiesel F, Moussaud Lamodière E, Ando M, Springer W. A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently. J Cell Sci. 2014;127:3488-504 pubmed publisher
  • immunohistochemistry - paraffin section; human
Satoh J, Motohashi N, Kino Y, Ishida T, Yagishita S, Jinnai K, et al. LC3, an autophagosome marker, is expressed on oligodendrocytes in Nasu-Hakola disease brains. Orphanet J Rare Dis. 2014;9:68 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100
Mori F, Watanabe Y, Miki Y, Tanji K, Odagiri S, Eto K, et al. Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases. Neuropathology. 2014;34:140-7 pubmed
  • western blot; human
He W, Wang Q, Srinivasan B, Xu J, Padilla M, Li Z, et al. A JNK-mediated autophagy pathway that triggers c-IAP degradation and necroptosis for anticancer chemotherapy. Oncogene. 2014;33:3004-13 pubmed publisher
  • western blot; human; fig 1a
Newman A, Scholefield C, Kemp A, Newman M, McIver E, Kamal A, et al. TBK1 kinase addiction in lung cancer cells is mediated via autophagy of Tax1bp1/Ndp52 and non-canonical NF-κB signalling. PLoS ONE. 2012;7:e50672 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100
Kon T, Mori F, Tanji K, Miki Y, Kimura T, Wakabayashi K. Giant cell polymyositis and myocarditis associated with myasthenia gravis and thymoma. Neuropathology. 2013;33:281-7 pubmed publisher
  • immunocytochemistry; human
Narita M, Young A, Arakawa S, Samarajiwa S, Nakashima T, Yoshida S, et al. Spatial coupling of mTOR and autophagy augments secretory phenotypes. Science. 2011;332:966-70 pubmed publisher
  • western blot; human
Yee K, Wilkinson S, James J, Ryan K, Vousden K. PUMA- and Bax-induced autophagy contributes to apoptosis. Cell Death Differ. 2009;16:1135-45 pubmed publisher
Zhu L, Retana D, García Gómez P, Álvaro Espinosa L, Priego N, Masmudi Martín M, et al. A clinically compatible drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis. EMBO Mol Med. 2022;14:e14552 pubmed publisher
Jung Y, Artan M, Kim N, Yeom J, Hwang A, Jeong D, et al. MON-2, a Golgi protein, mediates autophagy-dependent longevity in Caenorhabditis elegans. Sci Adv. 2021;7:eabj8156 pubmed publisher
Lee J, Kim J, Shin J, Kang Y, Choi J, Cheong H. ATG101 Degradation by HUWE1-Mediated Ubiquitination Impairs Autophagy and Reduces Survival in Cancer Cells. Int J Mol Sci. 2021;22: pubmed publisher
Trinkaus V, Riera Tur I, Martinez Sanchez A, Bäuerlein F, Guo Q, Arzberger T, et al. In situ architecture of neuronal α-Synuclein inclusions. Nat Commun. 2021;12:2110 pubmed publisher
Panier S, Maric M, Hewitt G, Mason Osann E, Gali H, Dai A, et al. SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance. Mol Cell. 2019;76:27-43.e11 pubmed publisher
Rockfield S, Kee Y, Nanjundan M. Chronic iron exposure and c-Myc/H-ras-mediated transformation in fallopian tube cells alter the expression of EVI1, amplified at 3q26.2 in ovarian cancer. Oncogenesis. 2019;8:46 pubmed publisher
Radhi O, Davidson S, Scott F, Zeng R, Jones D, Tomkinson N, et al. Inhibition of the ULK1 protein complex suppresses Staphylococcus-induced autophagy and cell death. J Biol Chem. 2019;294:14289-14307 pubmed publisher
Turco E, Witt M, Abert C, Bock Bierbaum T, Su M, Trapannone R, et al. FIP200 Claw Domain Binding to p62 Promotes Autophagosome Formation at Ubiquitin Condensates. Mol Cell. 2019;74:330-346.e11 pubmed publisher
Suvorova I, Knyazeva A, Petukhov A, Aksenov N, Pospelov V. Resveratrol enhances pluripotency of mouse embryonic stem cells by activating AMPK/Ulk1 pathway. Cell Death Discov. 2019;5:61 pubmed publisher
Mehto S, Jena K, Nath P, Chauhan S, Kolapalli S, Das S, et al. The Crohn's Disease Risk Factor IRGM Limits NLRP3 Inflammasome Activation by Impeding Its Assembly and by Mediating Its Selective Autophagy. Mol Cell. 2019;73:429-445.e7 pubmed publisher
Hu W, Zhang L, Li M, Shen J, Liu X, Xiao Z, et al. Vitamin D3 activates the autolysosomal degradation function against Helicobacter pylori through the PDIA3 receptor in gastric epithelial cells. Autophagy. 2019;:1-19 pubmed publisher
Karras P, Riveiro Falkenbach E, Cañón E, Tejedo C, Calvo T, Martínez Herranz R, et al. p62/SQSTM1 Fuels Melanoma Progression by Opposing mRNA Decay of a Selective Set of Pro-metastatic Factors. Cancer Cell. 2019;35:46-63.e10 pubmed publisher
Thomas H, Zhang Y, Stefely J, Veiga S, Thomas G, Kozma S, et al. Mitochondrial Complex I Activity Is Required for Maximal Autophagy. Cell Rep. 2018;24:2404-2417.e8 pubmed publisher
Puri C, Vicinanza M, Ashkenazi A, Gratian M, Zhang Q, Bento C, et al. The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A. Dev Cell. 2018;45:114-131.e8 pubmed publisher
Nwadike C, Williamson L, Gallagher L, Guan J, Chan E. AMPK Inhibits ULK1-Dependent Autophagosome Formation and Lysosomal Acidification via Distinct Mechanisms. Mol Cell Biol. 2018;38: pubmed publisher
Rockfield S, Flores I, Nanjundan M. Expression and function of nuclear receptor coactivator 4 isoforms in transformed endometriotic and malignant ovarian cells. Oncotarget. 2018;9:5344-5367 pubmed publisher
Mackenzie I, Nicholson A, Sarkar M, Messing J, Purice M, Pottier C, et al. TIA1 Mutations in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia Promote Phase Separation and Alter Stress Granule Dynamics. Neuron. 2017;95:808-816.e9 pubmed publisher
Liu L, He J, Wei X, Wan G, Lao Y, Xu W, et al. MicroRNA-20a-mediated loss of autophagy contributes to breast tumorigenesis by promoting genomic damage and instability. Oncogene. 2017;36:5874-5884 pubmed publisher
D Eliseo D, Di Renzo L, Santoni A, Velotti F. Docosahexaenoic acid (DHA) promotes immunogenic apoptosis in human multiple myeloma cells, induces autophagy and inhibits STAT3 in both tumor and dendritic cells. Genes Cancer. 2017;8:426-437 pubmed publisher
Sheu S, Chen J, Bee Y, Chen Y, Lin S, Shu C. Differential autophagic effects of vital dyes in retinal pigment epithelial ARPE-19 and photoreceptor 661W cells. PLoS ONE. 2017;12:e0174736 pubmed publisher
Cooper Knock J, Green C, Altschuler G, Wei W, Bury J, Heath P, et al. A data-driven approach links microglia to pathology and prognosis in amyotrophic lateral sclerosis. Acta Neuropathol Commun. 2017;5:23 pubmed publisher
Song C, Mitter S, Qi X, Beli E, Rao H, Ding J, et al. Oxidative stress-mediated NFκB phosphorylation upregulates p62/SQSTM1 and promotes retinal pigmented epithelial cell survival through increased autophagy. PLoS ONE. 2017;12:e0171940 pubmed publisher
Baranowska K, Misund K, Starheim K, Holien T, Johansson I, Darvekar S, et al. Hydroxychloroquine potentiates carfilzomib toxicity towards myeloma cells. Oncotarget. 2016;7:70845-70856 pubmed publisher
Riz I, Hawley T, Marsal J, Hawley R. Noncanonical SQSTM1/p62-Nrf2 pathway activation mediates proteasome inhibitor resistance in multiple myeloma cells via redox, metabolic and translational reprogramming. Oncotarget. 2016;7:66360-66385 pubmed publisher
Davis M, Delaney J, Patel C, Storgard R, Stupack D. Nelfinavir is effective against human cervical cancer cells in vivo: a potential treatment modality in resource-limited settings. Drug Des Devel Ther. 2016;10:1837-46 pubmed publisher
Panda P, Behera B, Meher B, Das D, Mukhopadhyay S, Sinha N, et al. Abrus Agglutinin, a type II ribosome inactivating protein inhibits Akt/PH domain to induce endoplasmic reticulum stress mediated autophagy-dependent cell death. Mol Carcinog. 2017;56:389-401 pubmed publisher
Plantinga T, Tesselaar M, Morreau H, Corssmit E, Willemsen B, Kusters B, et al. Autophagy activity is associated with membranous sodium iodide symporter expression and clinical response to radioiodine therapy in non-medullary thyroid cancer. Autophagy. 2016;12:1195-205 pubmed publisher
Gordeev S, Bykova T, Zubova S, Bystrova O, Martynova M, Pospelov V, et al. mTOR kinase inhibitor pp242 causes mitophagy terminated by apoptotic cell death in E1A-Ras transformed cells. Oncotarget. 2015;6:44905-26 pubmed publisher
Chandrika B, Yang C, Ou Y, Feng X, Muhoza D, Holmes A, et al. Endoplasmic Reticulum Stress-Induced Autophagy Provides Cytoprotection from Chemical Hypoxia and Oxidant Injury and Ameliorates Renal Ischemia-Reperfusion Injury. PLoS ONE. 2015;10:e0140025 pubmed publisher
Souquere S, Weil D, Pierron G. Comparative ultrastructure of CRM1-Nucleolar bodies (CNoBs), Intranucleolar bodies (INBs) and hybrid PML/p62 bodies uncovers new facets of nuclear body dynamic and diversity. Nucleus. 2015;6:326-38 pubmed publisher
Ayaki T, Ito H, Fukushima H, Inoue T, Kondo T, Ikemoto A, et al. Immunoreactivity of valosin-containing protein in sporadic amyotrophic lateral sclerosis and in a case of its novel mutant. Acta Neuropathol Commun. 2014;2:172 pubmed publisher
Caulfield T, Fiesel F, Moussaud Lamodière E, Dourado D, Flores S, Springer W. Phosphorylation by PINK1 releases the UBL domain and initializes the conformational opening of the E3 ubiquitin ligase Parkin. PLoS Comput Biol. 2014;10:e1003935 pubmed publisher
McCabe K, Bacos K, Lu D, Delaney J, Axelrod J, Potter M, et al. Triggering necroptosis in cisplatin and IAP antagonist-resistant ovarian carcinoma. Cell Death Dis. 2014;5:e1496 pubmed publisher
Shanware N, Bray K, Eng C, Wang F, Follettie M, Myers J, et al. Glutamine deprivation stimulates mTOR-JNK-dependent chemokine secretion. Nat Commun. 2014;5:4900 pubmed publisher
Assani K, Tazi M, Amer A, Kopp B. IFN-γ stimulates autophagy-mediated clearance of Burkholderia cenocepacia in human cystic fibrosis macrophages. PLoS ONE. 2014;9:e96681 pubmed publisher
Wan G, Xie W, Liu Z, Xu W, Lao Y, Huang N, et al. Hypoxia-induced MIR155 is a potent autophagy inducer by targeting multiple players in the MTOR pathway. Autophagy. 2014;10:70-9 pubmed publisher
Sorbye S, Kilvaer T, Valkov A, Donnem T, Smeland E, Al Shibli K, et al. Prognostic impact of Jab1, p16, p21, p62, Ki67 and Skp2 in soft tissue sarcomas. PLoS ONE. 2012;7:e47068 pubmed publisher
Tumbarello D, Waxse B, Arden S, Bright N, Kendrick Jones J, Buss F. Autophagy receptors link myosin VI to autophagosomes to mediate Tom1-dependent autophagosome maturation and fusion with the lysosome. Nat Cell Biol. 2012;14:1024-35 pubmed publisher
Stingele S, Stoehr G, Peplowska K, Cox J, Mann M, Storchova Z. Global analysis of genome, transcriptome and proteome reveals the response to aneuploidy in human cells. Mol Syst Biol. 2012;8:608 pubmed publisher
Satoh J, Tabunoki H, Ishida T, Saito Y, Arima K. Dystrophic neurites express C9orf72 in Alzheimer's disease brains. Alzheimers Res Ther. 2012;4:33 pubmed publisher
Fong J, Kells R, Gumpert A, Marzillier J, Davidson M, Falk M. Internalized gap junctions are degraded by autophagy. Autophagy. 2012;8:794-811 pubmed publisher
Melvin A, Mudie S, Rocha S. The chromatin remodeler ISWI regulates the cellular response to hypoxia: role of FIH. Mol Biol Cell. 2011;22:4171-81 pubmed publisher
Dortet L, Mostowy S, Samba Louaka A, Louaka A, Gouin E, Nahori M, et al. Recruitment of the major vault protein by InlK: a Listeria monocytogenes strategy to avoid autophagy. PLoS Pathog. 2011;7:e1002168 pubmed publisher
product information
Cat.# :
610832
Brand :
BD Transduction Laboratories
Name :
p62 Lck Ligand
Format :
Purified
Clone :
3/P62 LCK LIGAND
Isotype :
Ms IgG 1
React :
Hu , Ms , Rat
Conc :
250 µg/ml
Apps :
IF , WB
Size :
50 µg
Status :
RUO
more info or order :
company information