product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
Rac1
catalog :
610650
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
102/Rac1
reactivity :
human, mouse, rat, dogs, chicken
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, other, western blot knockout validation, immunocytochemistry knockout validation
more info or order :
citations: 58
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry knockout validation; mouse; 1:250; loading ...; fig 4b
  • other; mouse; 1:100; fig 3c
O Neil S, Racz B, Brown W, Gao Y, Soderblom E, Yasuda R, et al. Action potential-coupled Rho GTPase signaling drives presynaptic plasticity. elife. 2021;10: pubmed publisher
  • western blot knockout validation; mouse; loading ...; fig 3a
Ge J, Burnier L, Adamopoulou M, Kwa M, Schaks M, Rottner K, et al. RhoA, Rac1, and Cdc42 differentially regulate αSMA and collagen I expression in mesenchymal stem cells. J Biol Chem. 2018;293:9358-9369 pubmed publisher
  • western blot knockout validation; mouse; fig 1
Delaney M, Liu J, Zheng Y, Berndt M, Du X. The role of Rac1 in glycoprotein Ib-IX-mediated signal transduction and integrin activation. Arterioscler Thromb Vasc Biol. 2012;32:2761-8 pubmed publisher
  • western blot; human; 1:200; loading ...; fig 6h
Fayad R, Rojas M, Partisani M, Finetti P, Dib S, Abélanet S, et al. EFA6B regulates a stop signal for collective invasion in breast cancer. Nat Commun. 2021;12:2198 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:2000; loading ...; fig 4a
Nomura Komoike K, Saitoh F, Fujieda H. Phosphatidylserine recognition and Rac1 activation are required for Müller glia proliferation, gliosis and phagocytosis after retinal injury. Sci Rep. 2020;10:1488 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig e1g
Ramírez C, Hauser A, Vucic E, Bar Sagi D. Plasma membrane V-ATPase controls oncogenic RAS-induced macropinocytosis. Nature. 2019;576:477-481 pubmed publisher
  • western blot; human; loading ...; fig s1a
Ungefroren H, Otterbein H, Fiedler C, Mihara K, Hollenberg M, Gieseler F, et al. RAC1B Suppresses TGF-β1-Dependent Cell Migration in Pancreatic Carcinoma Cells through Inhibition of the TGF-β Type I Receptor ALK5. Cancers (Basel). 2019;11: pubmed publisher
  • western blot; mouse; loading ...; fig 6b
Lin C, Zhang Y, Zhang K, Zheng Y, Lu L, Chang H, et al. Fever Promotes T Lymphocyte Trafficking via a Thermal Sensory Pathway Involving Heat Shock Protein 90 and α4 Integrins. Immunity. 2019;50:137-151.e6 pubmed publisher
  • western blot; mouse; loading ...; fig 4c
Roy N, MacKay J, Robertson T, Hammer D, Burkhardt J. Crk adaptor proteins mediate actin-dependent T cell migration and mechanosensing induced by the integrin LFA-1. Sci Signal. 2018;11: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 3c
Polacheck W, Kutys M, Yang J, Eyckmans J, Wu Y, Vasavada H, et al. A non-canonical Notch complex regulates adherens junctions and vascular barrier function. Nature. 2017;552:258-262 pubmed publisher
  • western blot; human; loading ...; fig 3a
Barbiero I, Peroni D, Tramarin M, Chandola C, Rusconi L, Landsberger N, et al. The neurosteroid pregnenolone reverts microtubule derangement induced by the loss of a functional CDKL5-IQGAP1 complex. Hum Mol Genet. 2017;26:3520-3530 pubmed publisher
  • immunocytochemistry; human
  • western blot; human; loading ...; fig 1d
Yoon C, Cho S, Chang K, Park D, Ryeom S, Yoon S. Role of Rac1 Pathway in Epithelial-to-Mesenchymal Transition and Cancer Stem-like Cell Phenotypes in Gastric Adenocarcinoma. Mol Cancer Res. 2017;15:1106-1116 pubmed publisher
  • western blot; human; loading ...; fig 1b
Ruhe F, Olling A, Abromeit R, Rataj D, Grieschat M, Zeug A, et al. Overexpression of the Endosomal Anion/Proton Exchanger ClC-5 Increases Cell Susceptibility toward Clostridium difficile Toxins TcdA and TcdB. Front Cell Infect Microbiol. 2017;7:67 pubmed publisher
  • western blot; mouse; loading ...; fig 7D
Jeannot P, Nowosad A, Perchey R, Callot C, Bennana E, Katsube T, et al. p27Kip1 promotes invadopodia turnover and invasion through the regulation of the PAK1/Cortactin pathway. elife. 2017;6: pubmed publisher
  • western blot; human; loading ...; fig 6a
Eppler F, Quast T, Kolanus W. Dynamin2 controls Rap1 activation and integrin clustering in human T lymphocyte adhesion. PLoS ONE. 2017;12:e0172443 pubmed publisher
  • western blot; rat; loading ...; fig 3c
Lovric S, Gonçalves S, Gee H, Oskouian B, Srinivas H, Choi W, et al. Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency. J Clin Invest. 2017;127:912-928 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 6b
Rafiq N, Lieu Z, Jiang T, Yu C, Matsudaira P, Jones G, et al. Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO. J Cell Biol. 2017;216:181-197 pubmed publisher
  • western blot; human; loading ...; fig s3c
Tao L, Zhang J, Meraner P, Tovaglieri A, Wu X, Gerhard R, et al. Frizzled proteins are colonic epithelial receptors for C. difficile toxin B. Nature. 2016;538:350-355 pubmed publisher
  • western blot; human; loading ...; fig 5b
Frank S, Köllmann C, van Lidth de Jeude J, Thiagarajah J, Engelholm L, Frödin M, et al. The focal adhesion-associated proteins DOCK5 and GIT2 comprise a rheostat in control of epithelial invasion. Oncogene. 2017;36:1816-1828 pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig s4
Fusté N, Fernández Hernández R, Cemeli T, Mirantes C, Pedraza N, Rafel M, et al. Cytoplasmic cyclin D1 regulates cell invasion and metastasis through the phosphorylation of paxillin. Nat Commun. 2016;7:11581 pubmed publisher
  • western blot; mouse; fig 3A
Chen L, DeWispelaere A, Dastvan F, Osborne W, Blechner C, Windhorst S, et al. Smooth Muscle-Alpha Actin Inhibits Vascular Smooth Muscle Cell Proliferation and Migration by Inhibiting Rac1 Activity. PLoS ONE. 2016;11:e0155726 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 4
Takenaka N, Nihata Y, Satoh T. Rac1 Activation Caused by Membrane Translocation of a Guanine Nucleotide Exchange Factor in Akt2-Mediated Insulin Signaling in Mouse Skeletal Muscle. PLoS ONE. 2016;11:e0155292 pubmed publisher
  • immunocytochemistry; human; fig 7
  • western blot; human; fig 7
Marcos Ramiro B, García Weber D, Barroso S, Feito J, Ortega M, Cernuda Morollón E, et al. RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking to the cell border. J Cell Biol. 2016;213:385-402 pubmed publisher
  • western blot; mouse; 1:1000; fig 1
Marei H, Carpy A, Woroniuk A, Vennin C, White G, Timpson P, et al. Differential Rac1 signalling by guanine nucleotide exchange factors implicates FLII in regulating Rac1-driven cell migration. Nat Commun. 2016;7:10664 pubmed publisher
  • western blot; mouse; fig s3
Giampietro C, Disanza A, Bravi L, Barrios Rodiles M, Corada M, Frittoli E, et al. The actin-binding protein EPS8 binds VE-cadherin and modulates YAP localization and signaling. J Cell Biol. 2015;211:1177-92 pubmed publisher
  • flow cytometry; human; fig 8
  • immunocytochemistry; human; fig 3
Oprea T, Sklar L, Agola J, Guo Y, Silberberg M, Roxby J, et al. Novel Activities of Select NSAID R-Enantiomers against Rac1 and Cdc42 GTPases. PLoS ONE. 2015;10:e0142182 pubmed publisher
  • western blot; human
Fujimura K, Choi S, Wyse M, Strnadel J, Wright T, Klemke R. Eukaryotic Translation Initiation Factor 5A (EIF5A) Regulates Pancreatic Cancer Metastasis by Modulating RhoA and Rho-associated Kinase (ROCK) Protein Expression Levels. J Biol Chem. 2015;290:29907-19 pubmed publisher
  • western blot; human
Jamieson C, Lui C, Brocardo M, Martino Echarri E, Henderson B. Rac1 augments Wnt signaling by stimulating β-catenin-lymphoid enhancer factor-1 complex assembly independent of β-catenin nuclear import. J Cell Sci. 2015;128:3933-46 pubmed publisher
  • western blot; human; fig 5
He S, Zhao Z, Yang Y, O Connell D, Zhang X, Oh S, et al. Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers. Nat Commun. 2015;6:7839 pubmed publisher
  • western blot; human
Hahn C, Scott D, Xu X, Roda M, Payne G, Wells J, et al. The matrikine N-α-PGP couples extracellular matrix fragmentation to endothelial permeability. Sci Adv. 2015;1: pubmed
  • western blot; human; fig 7
Newell Litwa K, Badoual M, Asmussen H, Patel H, Whitmore L, Horwitz A. ROCK1 and 2 differentially regulate actomyosin organization to drive cell and synaptic polarity. J Cell Biol. 2015;210:225-42 pubmed publisher
  • western blot; human; 1:1000; fig 4
Whalley H, Porter A, Diamantopoulou Z, White G, Castañeda Saucedo E, Malliri A. Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation. Nat Commun. 2015;6:7437 pubmed publisher
  • western blot; human
Yamana S, Tokiyama A, Mizutani K, Hirata K, Takai Y, Rikitake Y. The Cell Adhesion Molecule Necl-4/CADM4 Serves as a Novel Regulator for Contact Inhibition of Cell Movement and Proliferation. PLoS ONE. 2015;10:e0124259 pubmed publisher
  • western blot; human; fig 1
Yuan P, Zhang H, Cai C, Zhu S, Zhou Y, Yang X, et al. Chondroitin sulfate proteoglycan 4 functions as the cellular receptor for Clostridium difficile toxin B. Cell Res. 2015;25:157-68 pubmed publisher
  • western blot; mouse; 1:1000
Delloye Bourgeois C, Jacquier A, Charoy C, Reynaud F, Nawabi H, Thoinet K, et al. PlexinA1 is a new Slit receptor and mediates axon guidance function of Slit C-terminal fragments. Nat Neurosci. 2015;18:36-45 pubmed publisher
  • immunoprecipitation; human
van Adrichem A, Fagerholm A, Turunen L, Lehto A, Saarela J, Koskinen A, et al. Discovery of MINC1, a GTPase-activating protein small molecule inhibitor, targeting MgcRacGAP. Comb Chem High Throughput Screen. 2015;18:3-17 pubmed
  • western blot; human
Kovacs I, Horvath M, Kovacs T, Somogyi K, Tretter L, Geiszt M, et al. Comparison of proton channel, phagocyte oxidase, and respiratory burst levels between human eosinophil and neutrophil granulocytes. Free Radic Res. 2014;48:1190-9 pubmed publisher
  • western blot; human; fig s1
Takeda A, Oberoi Khanuja T, Glatz G, Schulenburg K, Scholz R, Carpy A, et al. Ubiquitin-dependent regulation of MEKK2/3-MEK5-ERK5 signaling module by XIAP and cIAP1. EMBO J. 2014;33:1784-801 pubmed publisher
  • western blot; human
Zhong J, Bach C, Shum M, O Neill G. NEDD9 regulates 3D migratory activity independent of the Rac1 morphology switch in glioma and neuroblastoma. Mol Cancer Res. 2014;12:264-73 pubmed publisher
Luo W, Song Z, Sun H, Liang J, Zhao S. Bergamottin, a natural furanocoumarin abundantly present in grapefruit juice, suppresses the invasiveness of human glioma cells via inactivation of Rac1 signaling. Oncol Lett. 2018;15:3259-3266 pubmed publisher
Kroh H, Chandrasekaran R, Zhang Z, Rosenthal K, Woods R, Jin X, et al. A neutralizing antibody that blocks delivery of the enzymatic cargo of Clostridium difficile toxin TcdB into host cells. J Biol Chem. 2018;293:941-952 pubmed publisher
Zhang Y, Li J, Li B, Wang J, Liu C. Mycobacterium tuberculosis Mce3C promotes mycobacteria entry into macrophages through activation of ?2 integrin-mediated signalling pathway. Cell Microbiol. 2017;: pubmed publisher
Smith K, Rajgor D, Hanley J. Differential regulation of the Rac1 GTPase-activating protein (GAP) BCR during oxygen/glucose deprivation in hippocampal and cortical neurons. J Biol Chem. 2017;292:20173-20183 pubmed publisher
He R, Peng J, Yuan P, Yang J, Wu X, Wang Y, et al. Glucosyltransferase Activity of Clostridium difficile Toxin B Triggers Autophagy-mediated Cell Growth Arrest. Sci Rep. 2017;7:10532 pubmed publisher
Hernández Vásquez M, Adame García S, Hamoud N, Chidiac R, Reyes Cruz G, Gratton J, et al. Cell adhesion controlled by adhesion G protein-coupled receptor GPR124/ADGRA2 is mediated by a protein complex comprising intersectins and Elmo-Dock. J Biol Chem. 2017;292:12178-12191 pubmed publisher
Schelle I, Bruening J, Buetepage M, Genth H. Role of p38alpha/beta MAP Kinase in Cell Susceptibility to Clostridium sordellii Lethal Toxin and Clostridium difficile Toxin B. Toxins (Basel). 2016;9: pubmed publisher
Marei H, Carpy A, Macek B, Malliri A. Proteomic analysis of Rac1 signaling regulation by guanine nucleotide exchange factors. Cell Cycle. 2016;15:1961-74 pubmed publisher
Rocca D, Hanley J. PICK1 links AMPA receptor stimulation to Cdc42. Neurosci Lett. 2015;585:155-9 pubmed publisher
Cai K, Mulatz K, Ard R, Nguyen T, Gee S. Increased diacylglycerol kinase ? expression in human metastatic colon cancer cells augments Rho GTPase activity and contributes to enhanced invasion. BMC Cancer. 2014;14:208 pubmed publisher
Chen P, Schauer K, Kong C, Harding A, Goud B, Stahl P. Rab5 isoforms orchestrate a "division of labor" in the endocytic network; Rab5C modulates Rac-mediated cell motility. PLoS ONE. 2014;9:e90384 pubmed publisher
Ungefroren H, Sebens S, Giehl K, Helm O, Groth S, Fandrich F, et al. Rac1b negatively regulates TGF-?1-induced cell motility in pancreatic ductal epithelial cells by suppressing Smad signalling. Oncotarget. 2014;5:277-90 pubmed
Berger A, Hoelbl Kovacic A, Bourgeais J, Hoefling L, Warsch W, Grundschober E, et al. PAK-dependent STAT5 serine phosphorylation is required for BCR-ABL-induced leukemogenesis. Leukemia. 2014;28:629-41 pubmed publisher
Yefimova M, Messaddeq N, Harnois T, Meunier A, Clarhaut J, Noblanc A, et al. A chimerical phagocytosis model reveals the recruitment by Sertoli cells of autophagy for the degradation of ingested illegitimate substrates. Autophagy. 2013;9:653-66 pubmed publisher
Taniuchi K, Yokotani K, Saibara T. BART inhibits pancreatic cancer cell invasion by PKC? inactivation through binding to ANX7. PLoS ONE. 2012;7:e35674 pubmed publisher
Greciano P, Moyano J, Buschmann M, Tang J, Lu Y, Rudnicki J, et al. Laminin 511 partners with laminin 332 to mediate directional migration of Madin-Darby canine kidney epithelial cells. Mol Biol Cell. 2012;23:121-36 pubmed publisher
Vockel M, Pollok S, Breitenbach U, Ridderbusch I, Kreienkamp H, Brandner J. Somatostatin inhibits cell migration and reduces cell counts of human keratinocytes and delays epidermal wound healing in an ex vivo wound model. PLoS ONE. 2011;6:e19740 pubmed publisher
Khusial P, Vadla B, Krishnan H, Ramlall T, Shen Y, Ichikawa H, et al. Src activates Abl to augment Robo1 expression in order to promote tumor cell migration. Oncotarget. 2010;1:198-209 pubmed
Papatheodorou P, Zamboglou C, Genisyuerek S, Guttenberg G, Aktories K. Clostridial glucosylating toxins enter cells via clathrin-mediated endocytosis. PLoS ONE. 2010;5:e10673 pubmed publisher
product information
Cat.# :
610650
Brand :
BD Transduction Laboratories
Name :
Rac1
Format :
Purified
Clone :
102/Rac1
Isotype :
Ms IgG 2b
React :
Hu , Ms , Rat , Dog , Chick
Conc :
250 µg/ml
Apps :
IF , IHC , WB
Size :
50 µg
Status :
RUO
more info or order :
company information