product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
Nek2
catalog :
610593
quantity :
50 µg
price :
207.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
20/Nek2
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, other
more info or order :
BD Biosciences product webpage
citations: 10
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:500; loading ...; fig 1c, 2e
Allan L, Skowyra A, Rogers K, Zeller D, Clarke P. Atypical APC/C-dependent degradation of Mcl-1 provides an apoptotic timer during mitotic arrest. EMBO J. 2018;37: pubmed publisher
  • other; human; loading ...; fig st1
Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  • immunoprecipitation; human; fig 1
  • immunocytochemistry; human; 1:100; fig 2
  • western blot; human; 1:100; fig s11
Kim S, Lee K, Choi J, Ringstad N, Dynlacht B. Nek2 activation of Kif24 ensures cilium disassembly during the cell cycle. Nat Commun. 2015;6:8087 pubmed publisher
  • immunoprecipitation; human; 1:500
Boekhout M, Wolthuis R. Nek2A destruction marks APC/C activation at the prophase-to-prometaphase transition by spindle-checkpoint-restricted Cdc20. J Cell Sci. 2015;128:1639-53 pubmed publisher
  • western blot; human; 1:200; fig 2
Pagan J, Marzio A, Jones M, Saraf A, Jallepalli P, Florens L, et al. Degradation of Cep68 and PCNT cleavage mediate Cep215 removal from the PCM to allow centriole separation, disengagement and licensing. Nat Cell Biol. 2015;17:31-43 pubmed publisher
  • western blot; human
Lee M, Moreno C, Saavedra H. E2F activators signal and maintain centrosome amplification in breast cancer cells. Mol Cell Biol. 2014;34:2581-99 pubmed
  • western blot; human; 1:1000
Yu Y, Munger K. Human papillomavirus type 16 E7 oncoprotein inhibits the anaphase promoting complex/cyclosome activity by dysregulating EMI1 expression in mitosis. Virology. 2013;446:251-9 pubmed publisher
  • western blot; human
Harrison Pitner M, Saavedra H. Cdk4 and nek2 signal binucleation and centrosome amplification in a her2+ breast cancer model. PLoS ONE. 2013;8:e65971 pubmed publisher
Zeng X, King R. An APC/C inhibitor stabilizes cyclin B1 by prematurely terminating ubiquitination. Nat Chem Biol. 2012;8:383-92 pubmed publisher
Inanç B, Dodson H, Morrison C. A centrosome-autonomous signal that involves centriole disengagement permits centrosome duplication in G2 phase after DNA damage. Mol Biol Cell. 2010;21:3866-77 pubmed publisher
product information
Cat.# :
610593
Brand :
BD Transduction Laboratories
Name :
Nek2
Format :
Purified
Clone :
20/Nek2
Isotype :
Ms IgG 1
React :
Hu
Conc :
250 µg/ml
Apps :
IF , IHC(F)* , WB
Size :
50 µg
Status :
RUO
List Price :
207.00 USD
more info or order :
BD Biosciences product webpage
company information