product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
Clathrin Heavy Chain
catalog :
610499
quantity :
50 µg
price :
207.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
23/Clathrin Heavy Chain
reactivity :
human, mouse, rat, dog, chicken, pig
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 42
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; loading ...; fig s1e
Zhang Y, Tan L, Yang Q, Li C, Liou Y. The microtubule-associated protein HURP recruits the centrosomal protein TACC3 to regulate K-fiber formation and support chromosome congression. J Biol Chem. 2018;293:15733-15747 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 5e
Wang L, Chai Y, Li C, Liu H, Su W, Liu X, et al. Oxidized phospholipids are ligands for LRP6. Bone Res. 2018;6:22 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s3a
Vidal Quadras M, Holst M, Francis M, Larsson E, Hachimi M, Yau W, et al. Endocytic turnover of Rab8 controls cell polarization. J Cell Sci. 2017;130:1147-1157 pubmed publisher
  • western blot; human; loading ...; fig 1c
Van Puyenbroeck V, Claeys E, Schols D, Bell T, Vermeire K. A Proteomic Survey Indicates Sortilin as a Secondary Substrate of the ER Translocation Inhibitor Cyclotriazadisulfonamide (CADA). Mol Cell Proteomics. 2017;16:157-167 pubmed publisher
  • immunocytochemistry; pig; 1:50; loading ...; tbl 5
Démoulins T, Englezou P, Milona P, Ruggli N, Tirelli N, Pichon C, et al. Self-Replicating RNA Vaccine Delivery to Dendritic Cells. Methods Mol Biol. 2017;1499:37-75 pubmed
  • immunocytochemistry; human; fig 2
  • western blot; human; fig 3
Thomas R, Henson A, Gerrish A, Jones L, Williams J, Kidd E. Decreasing the expression of PICALM reduces endocytosis and the activity of β-secretase: implications for Alzheimer's disease. BMC Neurosci. 2016;17:50 pubmed publisher
  • western blot; pig; 1:1000; loading ...; fig 1c
Hernaez B, Guerra M, Salas M, Andres G. African Swine Fever Virus Undergoes Outer Envelope Disruption, Capsid Disassembly and Inner Envelope Fusion before Core Release from Multivesicular Endosomes. PLoS Pathog. 2016;12:e1005595 pubmed publisher
  • western blot; human; 1:3000; fig 2
Gschweitl M, Ulbricht A, Barnes C, Enchev R, Stoffel Studer I, Meyer Schaller N, et al. A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes. elife. 2016;5:e13841 pubmed publisher
  • western blot; human; 1:1000; tbl 4
Wollscheid H, Biancospino M, He F, Magistrati E, Molteni E, Lupia M, et al. Diverse functions of myosin VI elucidated by an isoform-specific α-helix domain. Nat Struct Mol Biol. 2016;23:300-308 pubmed publisher
  • immunocytochemistry; mouse; fig s4
  • western blot; mouse; fig s4
Bhattacharyya S, Rainey M, Arya P, Mohapatra B, Mushtaq I, Dutta S, et al. Endocytic recycling protein EHD1 regulates primary cilia morphogenesis and SHH signaling during neural tube development. Sci Rep. 2016;6:20727 pubmed publisher
  • western blot; rat; fig 1
Wang C, Wang Y, Hu M, Chai Z, Wu Q, Huang R, et al. Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis. EMBO Rep. 2016;17:47-63 pubmed publisher
  • immunocytochemistry; mouse; fig 5a
Yu C, Rafiq N, Cao F, Zhou Y, Krishnasamy A, Biswas K, et al. Integrin-beta3 clusters recruit clathrin-mediated endocytic machinery in the absence of traction force. Nat Commun. 2015;6:8672 pubmed publisher
  • western blot; human; fig s5
Moody P, Sayers E, Magnusson J, Alexander C, Borri P, Watson P, et al. Receptor Crosslinking: A General Method to Trigger Internalization and Lysosomal Targeting of Therapeutic Receptor:Ligand Complexes. Mol Ther. 2015;23:1888-98 pubmed publisher
  • immunocytochemistry; pig
Nimlamool W, Andrews R, Falk M. Connexin43 phosphorylation by PKC and MAPK signals VEGF-mediated gap junction internalization. Mol Biol Cell. 2015;26:2755-68 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 4
Kahra D, Mondol T, Niemiec M, Wittung Stafshede P. Human Copper Chaperone Atox1 Translocates to the Nucleus but does not Bind DNA In Vitro. Protein Pept Lett. 2015;22:532-8 pubmed
  • immunohistochemistry - paraffin section; mouse; 1:500
Tao W, Moore R, Smith E, Xu X. Hormonal induction and roles of Disabled-2 in lactation and involution. PLoS ONE. 2014;9:e110737 pubmed publisher
  • western blot; mouse
Zhang X, Lui W. Dysregulation of nectin-2 in the testicular cells: an explanation of cadmium-induced male infertility. Biochim Biophys Acta. 2014;1839:873-84 pubmed publisher
  • western blot; mouse; 1:1000
Bejarano E, Yuste A, Patel B, Stout R, Spray D, Cuervo A. Connexins modulate autophagosome biogenesis. Nat Cell Biol. 2014;16:401-14 pubmed publisher
  • western blot; human; 1:5000; fig 7
Sakane H, Horii Y, Nogami S, Kawano Y, Kaneko Kawano T, Shirataki H. ?-Taxilin interacts with sorting nexin 4 and participates in the recycling pathway of transferrin receptor. PLoS ONE. 2014;9:e93509 pubmed publisher
  • western blot; mouse; 1:2000
Fong J, Nimlamool W, Falk M. EGF induces efficient Cx43 gap junction endocytosis in mouse embryonic stem cell colonies via phosphorylation of Ser262, Ser279/282, and Ser368. FEBS Lett. 2014;588:836-44 pubmed publisher
  • western blot; rat; 1:10,000; fig 2d
Li P, Fu Z, Zhang Y, Zhang J, Xu C, Ma Y, et al. The clathrin adaptor Numb regulates intestinal cholesterol absorption through dynamic interaction with NPC1L1. Nat Med. 2014;20:80-6 pubmed publisher
  • western blot; human
Moravec R, Conger K, D Souza R, Allison A, Casanova J. BRAG2/GEP100/IQSec1 interacts with clathrin and regulates ?5?1 integrin endocytosis through activation of ADP ribosylation factor 5 (Arf5). J Biol Chem. 2012;287:31138-47 pubmed publisher
  • immunohistochemistry; human
Saghizadeh M, Kramerov A, Yaghoobzadeh Y, Hu J, Ljubimova J, Black K, et al. Adenovirus-driven overexpression of proteinases in organ-cultured normal human corneas leads to diabetic-like changes. Brain Res Bull. 2010;81:262-72 pubmed publisher
  • western blot; human
Piehl M, Lehmann C, Gumpert A, Denizot J, Segretain D, Falk M. Internalization of large double-membrane intercellular vesicles by a clathrin-dependent endocytic process. Mol Biol Cell. 2007;18:337-47 pubmed
  • western blot; human
Austin C, Lawrence D, Peden A, Varfolomeev E, Totpal K, De Mazière A, et al. Death-receptor activation halts clathrin-dependent endocytosis. Proc Natl Acad Sci U S A. 2006;103:10283-10288 pubmed publisher
Pascolutti R, Algisi V, Conte A, Raimondi A, Pasham M, Upadhyayula S, et al. Molecularly Distinct Clathrin-Coated Pits Differentially Impact EGFR Fate and Signaling. Cell Rep. 2019;27:3049-3061.e6 pubmed publisher
Lambert L, Dubayle D, Fafouri A, Herzog E, Csaba Z, Dournaud P, et al. Endocytosis of Activated Muscarinic m2 Receptor (m2R) in Live Mouse Hippocampal Neurons Occurs via a Clathrin-Dependent Pathway. Front Cell Neurosci. 2018;12:450 pubmed publisher
Nygård Skalman L, Holst M, Larsson E, Lundmark R. Plasma membrane damage caused by listeriolysin O is not repaired through endocytosis of the membrane pore. Biol Open. 2018;7: pubmed publisher
Ter Avetisyan G, Dumoulin A, Herrel A, Schmidt H, Strump J, Afzal S, et al. Loss of Axon Bifurcation in Mesencephalic Trigeminal Neurons Impairs the Maximal Biting Force in Npr2-Deficient Mice. Front Cell Neurosci. 2018;12:153 pubmed publisher
Langhorst H, Jüttner R, Groneberg D, Mohtashamdolatshahi A, Pelz L, Purfürst B, et al. The IgCAM CLMP regulates expression of Connexin43 and Connexin45 in intestinal and ureteral smooth muscle contraction in mice. Dis Model Mech. 2018;11: pubmed publisher
Thévenin A, Margraf R, Fisher C, Kells Andrews R, Falk M. Phosphorylation regulates connexin43/ZO-1 binding and release, an important step in gap junction turnover. Mol Biol Cell. 2017;28:3595-3608 pubmed publisher
Daste F, Walrant A, Holst M, Gadsby J, Mason J, Lee J, et al. Control of actin polymerization via the coincidence of phosphoinositides and high membrane curvature. J Cell Biol. 2017;216:3745-3765 pubmed publisher
Beautrait A, Paradis J, Zimmerman B, Giubilaro J, Nikolajev L, Armando S, et al. A new inhibitor of the β-arrestin/AP2 endocytic complex reveals interplay between GPCR internalization and signalling. Nat Commun. 2017;8:15054 pubmed publisher
Francis M, Holst M, Vidal Quadras M, Henriksson S, Santarella Mellwig R, Sandblad L, et al. Endocytic membrane turnover at the leading edge is driven by a transient interaction between Cdc42 and GRAF1. J Cell Sci. 2015;128:4183-95 pubmed publisher
Boassa D, Nguyen P, Hu J, Ellisman M, Sosinsky G. Pannexin2 oligomers localize in the membranes of endosomal vesicles in mammalian cells while Pannexin1 channels traffic to the plasma membrane. Front Cell Neurosci. 2014;8:468 pubmed publisher
Fong J, Kells R, Falk M. Two tyrosine-based sorting signals in the Cx43 C-terminus cooperate to mediate gap junction endocytosis. Mol Biol Cell. 2013;24:2834-48 pubmed publisher
Zlatic S, Grossniklaus E, Ryder P, Salazar G, Mattheyses A, Peden A, et al. Chemical-genetic disruption of clathrin function spares adaptor complex 3-dependent endosome vesicle biogenesis. Mol Biol Cell. 2013;24:2378-88 pubmed publisher
Gao Y, Lui W. Transforming growth factor-?1 (TGF-?1) regulates cell junction restructuring via Smad-mediated repression and clathrin-mediated endocytosis of nectin-like molecule 2 (Necl-2). PLoS ONE. 2013;8:e64316 pubmed publisher
Yamada K, Hayashi M, Madokoro H, Nishida H, Du W, Ohnuma K, et al. Nuclear localization of CD26 induced by a humanized monoclonal antibody inhibits tumor cell growth by modulating of POLR2A transcription. PLoS ONE. 2013;8:e62304 pubmed publisher
Roberts R, Al Jamal W, Whelband M, Thomas P, Jefferson M, Van den Bossche J, et al. Autophagy and formation of tubulovesicular autophagosomes provide a barrier against nonviral gene delivery. Autophagy. 2013;9:667-82 pubmed publisher
Rice W, Zhang Y, Chen Y, Matsuzaki T, Brown D, Lu H. Differential, phosphorylation dependent trafficking of AQP2 in LLC-PK1 cells. PLoS ONE. 2012;7:e32843 pubmed publisher
Papatheodorou P, Zamboglou C, Genisyuerek S, Guttenberg G, Aktories K. Clostridial glucosylating toxins enter cells via clathrin-mediated endocytosis. PLoS ONE. 2010;5:e10673 pubmed publisher
product information
Cat.# :
610499
Brand :
BD Transduction Laboratories
Name :
Clathrin Heavy Chain
Format :
Purified
Clone :
23/Clathrin Heavy Chain
Isotype :
Ms IgG 1
React :
Hu , Ms , Rat , Dog , Chick
Conc :
250 µg/ml
Apps :
Bioimg , IF , IHC , IP , WB
Size :
50 µg
Status :
RUO
List Price :
207.00 USD
more info or order :
company information