Published Application/Species/Sample/Dilution | Reference |
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- western blot knockout validation; mouse; loading ...; fig 3c
| Wang H, Meng H, Li X, Zhu K, Dong K, Mookhtiar A, et al. PELI1 functions as a dual modulator of necroptosis and apoptosis by regulating ubiquitination of RIPK1 and mRNA levels of c-FLIP. Proc Natl Acad Sci U S A. 2017;114:11944-11949 pubmed publisher
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- western blot knockout validation; mouse; loading ...; fig 1h
| Van T, Polykratis A, Straub B, Kondylis V, Papadopoulou N, Pasparakis M. Kinase-independent functions of RIPK1 regulate hepatocyte survival and liver carcinogenesis. J Clin Invest. 2017;127:2662-2677 pubmed publisher
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- western blot knockout validation; mouse; loading ...; fig s4a
- immunoprecipitation; mouse; loading ...; fig 2a
| Newton K, Wickliffe K, Maltzman A, Dugger D, Strasser A, Pham V, et al. RIPK1 inhibits ZBP1-driven necroptosis during development. Nature. 2016;540:129-133 pubmed publisher
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- western blot knockout validation; mouse; fig 9
| Kang S, Fernandes Alnemri T, Rogers C, Mayes L, Wang Y, Dillon C, et al. Caspase-8 scaffolding function and MLKL regulate NLRP3 inflammasome activation downstream of TLR3. Nat Commun. 2015;6:7515 pubmed publisher
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- immunohistochemistry; human; loading ...; fig 2b
| Lomphithak T, Akara Amornthum P, Murakami K, Hashimoto M, Usubuchi H, Iwabuchi E, et al. Tumor necroptosis is correlated with a favorable immune cell signature and programmed death-ligand 1 expression in cholangiocarcinoma. Sci Rep. 2021;11:11743 pubmed publisher
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- immunohistochemistry - paraffin section; rat; 1:200; loading ...; fig 1k
- immunocytochemistry; rat; 1:200; loading ...; fig 1m
- western blot; rat; 1:1000; loading ...; fig 2e
| Cheng J, Duan X, Fu X, Jiang Y, Yang P, Cao C, et al. RIP1 Perturbation Induces Chondrocyte Necroptosis and Promotes Osteoarthritis Pathogenesis via Targeting BMP7. Front Cell Dev Biol. 2021;9:638382 pubmed publisher
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- immunocytochemistry; human; loading ...; fig 4a, 4b
- western blot; human; loading ...; fig 5a, 5c
| Hu H, Wu G, Shu Z, Yu D, Nan N, Yuan F, et al. ICP6 Prevents RIP1 Activation to Hinder Necroptosis Signaling. Front Cell Dev Biol. 2020;8:595253 pubmed publisher
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- western blot; human; loading ...; fig 3d
| Lomphithak T, Choksi S, Mutirangura A, Tohtong R, Tencomnao T, Usubuchi H, et al. Receptor-interacting protein kinase 1 is a key mediator in TLR3 ligand and Smac mimetic-induced cell death and suppresses TLR3 ligand-promoted invasion in cholangiocarcinoma. Cell Commun Signal. 2020;18:161 pubmed publisher
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- western blot; human; 1:6000; loading ...; fig 2c
| Wu Y, Chou T, Young L, Hsieh F, Pan H, Mo S, et al. Tumor suppressor death-associated protein kinase 1 inhibits necroptosis by p38 MAPK activation. Cell Death Dis. 2020;11:305 pubmed publisher
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- immunohistochemistry; mouse; 1:200; loading ...; fig s12c
- western blot; mouse; 1:1000; loading ...; fig s12b
| Tanaka H, Homma H, Fujita K, Kondo K, Yamada S, Jin X, et al. YAP-dependent necrosis occurs in early stages of Alzheimer's disease and regulates mouse model pathology. Nat Commun. 2020;11:507 pubmed publisher
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- western blot; human; fig 1h
| Lalaoui N, Boyden S, Oda H, Wood G, Stone D, Chau D, et al. Mutations that prevent caspase cleavage of RIPK1 cause autoinflammatory disease. Nature. 2020;577:103-108 pubmed publisher
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- western blot; mouse; loading ...; fig 3d
| Lim J, Park H, Heisler J, Maculins T, Roose Girma M, Xu M, et al. Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins. elife. 2019;8: pubmed publisher
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- western blot; mouse; loading ...; fig 4c
| Krishna Subramanian S, Singer S, Armaka M, Banales J, Hölzer K, Schirmacher P, et al. RIPK1 and death receptor signaling drive biliary damage and early liver tumorigenesis in mice with chronic hepatobiliary injury. Cell Death Differ. 2019;: pubmed publisher
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- western blot; mouse; 1:1000; loading ...; fig 3i
| Frank T, Tuppi M, Hugle M, Dötsch V, van Wijk S, Fulda S. Cell cycle arrest in mitosis promotes interferon-induced necroptosis. Cell Death Differ. 2019;: pubmed publisher
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- western blot; mouse; 1:500; loading ...; fig 4g
| Daruich A, Le Rouzic Q, Jonet L, Naud M, Kowalczuk L, Pournaras J, et al. Iron is neurotoxic in retinal detachment and transferrin confers neuroprotection. Sci Adv. 2019;5:eaau9940 pubmed publisher
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- proximity ligation assay; mouse; 1:50; loading ...; fig 1f
- western blot; mouse; 1:1000; loading ...; fig 1b
- proximity ligation assay; human; 1:50; loading ...; fig 1d
- immunoprecipitation; human; loading ...; fig 2a
- western blot; human; 1:1000; loading ...; fig 1a
| Liccardi G, Ramos Garcia L, Tenev T, Annibaldi A, Legrand A, Robertson D, et al. RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation. Mol Cell. 2019;73:413-428.e7 pubmed publisher
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- western blot; mouse; loading ...; fig 3b
| Siegmund D, Ehrenschwender M, Wajant H. TNFR2 unlocks a RIPK1 kinase activity-dependent mode of proinflammatory TNFR1 signaling. Cell Death Dis. 2018;9:921 pubmed publisher
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- western blot; mouse; loading ...; fig 2b
| Peltzer N, Darding M, Montinaro A, Dráber P, Draberova H, Kupka S, et al. LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis. Nature. 2018;557:112-117 pubmed publisher
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- western blot; mouse; loading ...; fig 6g
- western blot; human; loading ...; fig 8b
| Lafont E, Kantari Mimoun C, Dráber P, De Miguel D, Hartwig T, Reichert M, et al. The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death. EMBO J. 2017;36:1147-1166 pubmed publisher
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- immunoprecipitation; mouse; fig 4
- western blot; mouse; fig 1
| Lin J, Kumari S, Kim C, Van T, Wachsmuth L, Polykratis A, et al. RIPK1 counteracts ZBP1-mediated necroptosis to inhibit inflammation. Nature. 2016;540:124-128 pubmed publisher
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- western blot; human; fig 1
| Kupka S, De Miguel D, Dráber P, Martino L, Surinova S, Rittinger K, et al. SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes. Cell Rep. 2016;16:2271-80 pubmed publisher
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- western blot; mouse; fig 5
| Tortola L, Nitsch R, Bertrand M, Kogler M, Redouane Y, Kozieradzki I, et al. The Tumor Suppressor Hace1 Is a Critical Regulator of TNFR1-Mediated Cell Fate. Cell Rep. 2016;15:1481-1492 pubmed publisher
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- western blot; human; fig 2
| Emmerich C, Bakshi S, Kelsall I, Ortiz Guerrero J, Shpiro N, Cohen P. Lys63/Met1-hybrid ubiquitin chains are commonly formed during the activation of innate immune signalling. Biochem Biophys Res Commun. 2016;474:452-461 pubmed publisher
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- western blot; mouse; fig 1
| Aaes T, Kaczmarek A, Delvaeye T, De Craene B, De Koker S, Heyndrickx L, et al. Vaccination with Necroptotic Cancer Cells Induces Efficient Anti-tumor Immunity. Cell Rep. 2016;15:274-87 pubmed publisher
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- western blot; mouse; fig 6j
| Vlantis K, Wullaert A, Polykratis A, Kondylis V, Dannappel M, Schwarzer R, et al. NEMO Prevents RIP Kinase 1-Mediated Epithelial Cell Death and Chronic Intestinal Inflammation by NF-κB-Dependent and -Independent Functions. Immunity. 2016;44:553-567 pubmed publisher
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- proximity ligation assay; human; fig 7
- western blot; human; 1:1000; fig 6
- proximity ligation assay; mouse; fig 7
- western blot; mouse; 1:1000; fig 8
| Orme M, Liccardi G, Moderau N, Feltham R, Wicky John S, Tenev T, et al. The unconventional myosin CRINKLED and its mammalian orthologue MYO7A regulate caspases in their signalling roles. Nat Commun. 2016;7:10972 pubmed publisher
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- western blot; mouse; fig 7
| Thornton T, Delgado P, Chen L, Salas B, Krementsov D, Fernández M, et al. Inactivation of nuclear GSK3β by Ser(389) phosphorylation promotes lymphocyte fitness during DNA double-strand break response. Nat Commun. 2016;7:10553 pubmed publisher
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- other; human; loading ...; fig st1
| Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
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- western blot; rat; 1:1000; fig 3
| Zhang T, Zhang Y, Cui M, Jin L, Wang Y, Lv F, et al. CaMKII is a RIP3 substrate mediating ischemia- and oxidative stress-induced myocardial necroptosis. Nat Med. 2016;22:175-82 pubmed publisher
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- immunocytochemistry; human; fig s9
- western blot; human; fig 5b
| Kharaziha P, Chioureas D, Baltatzis G, Fonseca P, Rodriguez P, Gogvadze V, et al. Sorafenib-induced defective autophagy promotes cell death by necroptosis. Oncotarget. 2015;6:37066-82 pubmed publisher
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- western blot; mouse
- western blot; human
| Yoon S, Bogdanov K, Kovalenko A, Wallach D. Necroptosis is preceded by nuclear translocation of the signaling proteins that induce it. Cell Death Differ. 2016;23:253-60 pubmed publisher
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- western blot; mouse; loading ...; fig 3f
| Boutaffala L, Bertrand M, Remouchamps C, Seleznik G, Reisinger F, Janas M, et al. NIK promotes tissue destruction independently of the alternative NF-κB pathway through TNFR1/RIP1-induced apoptosis. Cell Death Differ. 2015;22:2020-33 pubmed publisher
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- western blot; human; fig 4a
| Philipp S, Sosna J, Plenge J, Kalthoff H, Adam D. Homoharringtonine, a clinically approved anti-leukemia drug, sensitizes tumor cells for TRAIL-induced necroptosis. Cell Commun Signal. 2015;13:25 pubmed publisher
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- western blot; mouse; 1:2000; fig 4
| Ko R, Park J, Ha H, Choi Y, Lee S. Glycogen synthase kinase 3β ubiquitination by TRAF6 regulates TLR3-mediated pro-inflammatory cytokine production. Nat Commun. 2015;6:6765 pubmed publisher
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- western blot; mouse; 1:1000; fig 2
| Dowling J, Nair A, Zhang J. A novel function of RIP1 in postnatal development and immune homeostasis by protecting against RIP3-dependent necroptosis and FADD-mediated apoptosis. Front Cell Dev Biol. 2015;3:12 pubmed publisher
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- western blot; mouse; 1:1000; fig 5
| Chen W, Wu J, Li L, Zhang Z, Ren J, Liang Y, et al. Ppm1b negatively regulates necroptosis through dephosphorylating Rip3. Nat Cell Biol. 2015;17:434-44 pubmed publisher
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- western blot; mouse; fig 2
| Saveljeva S, Mc Laughlin S, Vandenabeele P, Samali A, Bertrand M. Endoplasmic reticulum stress induces ligand-independent TNFR1-mediated necroptosis in L929 cells. Cell Death Dis. 2015;6:e1587 pubmed publisher
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- western blot; mouse; fig 3
| Dannappel M, Vlantis K, Kumari S, Polykratis A, Kim C, Wachsmuth L, et al. RIPK1 maintains epithelial homeostasis by inhibiting apoptosis and necroptosis. Nature. 2014;513:90-4 pubmed publisher
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| Liu S, Wang X, Li Y, Xu L, Yu X, Ge L, et al. Necroptosis mediates TNF-induced toxicity of hippocampal neurons. Biomed Res Int. 2014;2014:290182 pubmed publisher
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| Elmallah M, Cogo S, Constantinescu A, Elifio Esposito S, Abdelfattah M, Micheau O. Marine Actinomycetes-Derived Secondary Metabolites Overcome TRAIL-Resistance via the Intrinsic Pathway through Downregulation of Survivin and XIAP. Cells. 2020;9: pubmed publisher
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| Schwarzer R, Jiao H, Wachsmuth L, Tresch A, Pasparakis M. FADD and Caspase-8 Regulate Gut Homeostasis and Inflammation by Controlling MLKL- and GSDMD-Mediated Death of Intestinal Epithelial Cells. Immunity. 2020;52:978-993.e6 pubmed publisher
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| Xu X, Kalac M, Markson M, Chan M, Brody J, Bhagat G, et al. Reversal of CYLD phosphorylation as a novel therapeutic approach for adult T-cell leukemia/lymphoma (ATLL). Cell Death Dis. 2020;11:94 pubmed publisher
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| Gomez Giro G, Arias Fuenzalida J, Jarazo J, Zeuschner D, Ali M, Possemis N, et al. Synapse alterations precede neuronal damage and storage pathology in a human cerebral organoid model of CLN3-juvenile neuronal ceroid lipofuscinosis. Acta Neuropathol Commun. 2019;7:222 pubmed publisher
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| Zhang X, Zhang H, Xu C, Li X, Li M, Wu X, et al. Ubiquitination of RIPK1 suppresses programmed cell death by regulating RIPK1 kinase activation during embryogenesis. Nat Commun. 2019;10:4158 pubmed publisher
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| Cao M, Chen F, Xie N, Cao M, Chen P, Lou Q, et al. c-Jun N-terminal kinases differentially regulate TNF- and TLRs-mediated necroptosis through their kinase-dependent and -independent activities. Cell Death Dis. 2018;9:1140 pubmed publisher
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| Smith J, Zhang Y, Li J, McCabe A, Jo H, Maloney J, et al. Type I IFNs drive hematopoietic stem and progenitor cell collapse via impaired proliferation and increased RIPK1-dependent cell death during shock-like ehrlichial infection. PLoS Pathog. 2018;14:e1007234 pubmed publisher
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| Fuchslocher Chico J, Falk Paulsen M, Luzius A, Saggau C, Ruder B, Bolik J, et al. The enhanced susceptibility of ADAM-17 hypomorphic mice to DSS-induced colitis is not ameliorated by loss of RIPK3, revealing an unexpected function of ADAM-17 in necroptosis. Oncotarget. 2018;9:12941-12958 pubmed publisher
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| Annibaldi A, Wicky John S, Vanden Berghe T, Swatek K, Ruan J, Liccardi G, et al. Ubiquitin-Mediated Regulation of RIPK1 Kinase Activity Independent of IKK and MK2. Mol Cell. 2018;69:566-580.e5 pubmed publisher
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| Stutz M, Ojaimi S, Allison C, Preston S, Arandjelovic P, Hildebrand J, et al. Necroptotic signaling is primed in Mycobacterium tuberculosis-infected macrophages, but its pathophysiological consequence in disease is restricted. Cell Death Differ. 2018;25:951-965 pubmed publisher
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| Ishii T, Okai T, Iwatani Yoshihara M, Mochizuki M, Unno S, Kuno M, et al. CETSA quantitatively verifies in vivo target engagement of novel RIPK1 inhibitors in various biospecimens. Sci Rep. 2017;7:13000 pubmed publisher
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| Zhang Y, Su S, Zhao S, Yang Z, Zhong C, Chen X, et al. RIP1 autophosphorylation is promoted by mitochondrial ROS and is essential for RIP3 recruitment into necrosome. Nat Commun. 2017;8:14329 pubmed publisher
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| Wen S, Ling Y, Yang W, Shen J, Li C, Deng W, et al. Necroptosis is a key mediator of enterocytes loss in intestinal ischaemia/reperfusion injury. J Cell Mol Med. 2017;21:432-443 pubmed publisher
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| Estornes Y, Aguileta M, Dubuisson C, De Keyser J, Goossens V, Kersse K, et al. RIPK1 promotes death receptor-independent caspase-8-mediated apoptosis under unresolved ER stress conditions. Cell Death Dis. 2014;5:e1555 pubmed publisher
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| Remijsen Q, Goossens V, Grootjans S, Van den Haute C, Vanlangenakker N, Dondelinger Y, et al. Depletion of RIPK3 or MLKL blocks TNF-driven necroptosis and switches towards a delayed RIPK1 kinase-dependent apoptosis. Cell Death Dis. 2014;5:e1004 pubmed publisher
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| Choi J, Devkota S, Sung Y, Lee H. EI24 regulates epithelial-to-mesenchymal transition and tumor progression by suppressing TRAF2-mediated NF-?B activity. Oncotarget. 2013;4:2383-96 pubmed
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| Vanlangenakker N, Bertrand M, Bogaert P, Vandenabeele P, Vanden Berghe T. TNF-induced necroptosis in L929 cells is tightly regulated by multiple TNFR1 complex I and II members. Cell Death Dis. 2011;2:e230 pubmed publisher
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| Nagabhushana A, Bansal M, Swarup G. Optineurin is required for CYLD-dependent inhibition of TNF?-induced NF-?B activation. PLoS ONE. 2011;6:e17477 pubmed publisher
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