product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
RIP
catalog :
610458
quantity :
50 µg
price :
207.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
38/RIP
reactivity :
human, mouse, rat, dogs, chicken
application :
western blot, immunocytochemistry, immunoprecipitation, other, western blot knockout validation
more info or order :
citations: 44
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 3d
  • western blot knockout validation; human; loading ...; fig 4d
Tanzer M, Khan N, Rickard J, Etemadi N, Lalaoui N, Spall S, et al. Combination of IAP antagonist and IFNγ activates novel caspase-10- and RIPK1-dependent cell death pathways. Cell Death Differ. 2017;24:481-491 pubmed publisher
  • western blot knockout validation; human; 1:1000; loading ...; fig 4b
Guicciardi M, Krishnan A, Bronk S, Hirsova P, Griffith T, Gores G. Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice. Cell Death Dis. 2017;8:e2535 pubmed publisher
  • western blot; mouse; loading ...; fig s7e
Yang S, Harding A, Sweeney C, Miao D, Swan G, Zhou C, et al. Control of antiviral innate immune response by protein geranylgeranylation. Sci Adv. 2019;5:eaav7999 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 4g
Daruich A, Le Rouzic Q, Jonet L, Naud M, Kowalczuk L, Pournaras J, et al. Iron is neurotoxic in retinal detachment and transferrin confers neuroprotection. Sci Adv. 2019;5:eaau9940 pubmed publisher
  • western blot; human; fig 3f
Fauster A, Rebsamen M, Willmann K, César Razquin A, Girardi E, Bigenzahn J, et al. Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking. Cell Death Differ. 2019;26:1138-1155 pubmed publisher
  • western blot; human; loading ...; fig s3
Hartwig T, Montinaro A, von Karstedt S, Sevko A, Surinova S, Chakravarthy A, et al. The TRAIL-Induced Cancer Secretome Promotes a Tumor-Supportive Immune Microenvironment via CCR2. Mol Cell. 2017;65:730-742.e5 pubmed publisher
  • western blot; human; loading ...; fig s1b
Samson A, Ho B, Au A, Schoenwaelder S, Smyth M, Bottomley S, et al. Physicochemical properties that control protein aggregation also determine whether a protein is retained or released from necrotic cells. Open Biol. 2016;6: pubmed
  • western blot; human; 1:1000; fig s15
Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, et al. Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. Nat Commun. 2016;7:12547 pubmed publisher
  • western blot; human; fig 5
Wagner S, Satpathy S, Beli P, Choudhary C. SPATA2 links CYLD to the TNF-? receptor signaling complex and modulates the receptor signaling outcomes. EMBO J. 2016;35:1868-84 pubmed publisher
  • immunoprecipitation; mouse; loading ...; fig s1
  • western blot; mouse; loading ...; fig 2a
Shutinoski B, Alturki N, Rijal D, Bertin J, Gough P, Schlossmacher M, et al. K45A mutation of RIPK1 results in poor necroptosis and cytokine signaling in macrophages, which impacts inflammatory responses in vivo. Cell Death Differ. 2016;23:1628-37 pubmed publisher
  • other; human; loading ...; fig st1
Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  • western blot; mouse; fig 3
Jacobsen A, Lowes K, Tanzer M, Lucet I, Hildebrand J, Petrie E, et al. HSP90 activity is required for MLKL oligomerisation and membrane translocation and the induction of necroptotic cell death. Cell Death Dis. 2016;7:e2051 pubmed publisher
  • western blot; mouse; 1:500; fig 6
Ogura Y, Hindi S, Sato S, Xiong G, Akira S, Kumar A. TAK1 modulates satellite stem cell homeostasis and skeletal muscle repair. Nat Commun. 2015;6:10123 pubmed publisher
  • western blot; human
Fauster A, Rebsamen M, Huber K, Bigenzahn J, Stukalov A, Lardeau C, et al. A cellular screen identifies ponatinib and pazopanib as inhibitors of necroptosis. Cell Death Dis. 2015;6:e1767 pubmed publisher
  • western blot; human; loading ...; fig 1a,2a,3
Selmi T, Alecci C, dell Aquila M, Montorsi L, Martello A, Guizzetti F, et al. ZFP36 stabilizes RIP1 via degradation of XIAP and cIAP2 thereby promoting ripoptosome assembly. BMC Cancer. 2015;15:357 pubmed publisher
  • western blot; human
Yanagi T, Shi R, Aza Blanc P, Reed J, Matsuzawa S. PCTAIRE1-knockdown sensitizes cancer cells to TNF family cytokines. PLoS ONE. 2015;10:e0119404 pubmed publisher
  • western blot; mouse; 1:1000; fig 4e
Lawlor K, Khan N, Mildenhall A, Gerlic M, Croker B, D Cruz A, et al. RIPK3 promotes cell death and NLRP3 inflammasome activation in the absence of MLKL. Nat Commun. 2015;6:6282 pubmed publisher
  • western blot; human
Shi S, Wang Q, Xu J, Jang J, Padilla M, Nyunoya T, et al. Synergistic anticancer effect of cisplatin and Chal-24 combination through IAP and c-FLIPL degradation, Ripoptosome formation and autophagy-mediated apoptosis. Oncotarget. 2015;6:1640-51 pubmed
  • western blot; human
Wang Y, Tan B, Mu R, Chang Y, Wu M, Tu H, et al. Ubiquitin-associated domain-containing ubiquitin regulatory X (UBX) protein UBXN1 is a negative regulator of nuclear factor κB (NF-κB) signaling. J Biol Chem. 2015;290:10395-405 pubmed publisher
  • immunoprecipitation; mouse; fig 2a
  • western blot; mouse; fig 2d
Chen G, Cheng X, Zhao M, Lin S, Lu J, Kang J, et al. RIP1-dependent Bid cleavage mediates TNFα-induced but Caspase-3-independent cell death in L929 fibroblastoma cells. Apoptosis. 2015;20:92-109 pubmed publisher
  • western blot; mouse
McComb S, Shutinoski B, Thurston S, Cessford E, Kumar K, Sad S. Cathepsins limit macrophage necroptosis through cleavage of Rip1 kinase. J Immunol. 2014;192:5671-8 pubmed publisher
  • western blot; human
Wang Q, Shi S, He W, Padilla M, Zhang L, Wang X, et al. Retaining MKP1 expression and attenuating JNK-mediated apoptosis by RIP1 for cisplatin resistance through miR-940 inhibition. Oncotarget. 2014;5:1304-14 pubmed
  • western blot; human
He W, Wang Q, Srinivasan B, Xu J, Padilla M, Li Z, et al. A JNK-mediated autophagy pathway that triggers c-IAP degradation and necroptosis for anticancer chemotherapy. Oncogene. 2014;33:3004-13 pubmed publisher
  • western blot; human
Han J, Soletti R, Sadarangani A, Sridevi P, Ramirez M, Eckmann L, et al. Nuclear expression of ?-catenin promotes RB stability and resistance to TNF-induced apoptosis in colon cancer cells. Mol Cancer Res. 2013;11:207-18 pubmed publisher
Wang Z, Feng J, Yu J, Chen G. FKBP12 mediates necroptosis by initiating RIPK1-RIPK3-MLKL signal transduction in response to TNF receptor 1 ligation. J Cell Sci. 2019;132: pubmed publisher
Smith J, Zhang Y, Li J, McCabe A, Jo H, Maloney J, et al. Type I IFNs drive hematopoietic stem and progenitor cell collapse via impaired proliferation and increased RIPK1-dependent cell death during shock-like ehrlichial infection. PLoS Pathog. 2018;14:e1007234 pubmed publisher
Kabashima A, Hirsova P, Bronk S, Hernandez M, Truty M, Rizvi S, et al. Fibroblast growth factor receptor inhibition induces loss of matrix MCL1 and necrosis in cholangiocarcinoma. J Hepatol. 2018;68:1228-1238 pubmed publisher
Stutz M, Ojaimi S, Allison C, Preston S, Arandjelovic P, Hildebrand J, et al. Necroptotic signaling is primed in Mycobacterium tuberculosis-infected macrophages, but its pathophysiological consequence in disease is restricted. Cell Death Differ. 2018;25:951-965 pubmed publisher
Van Opdenbosch N, Van Gorp H, Verdonckt M, Saavedra P, de Vasconcelos N, Gonçalves A, et al. Caspase-1 Engagement and TLR-Induced c-FLIP Expression Suppress ASC/Caspase-8-Dependent Apoptosis by Inflammasome Sensors NLRP1b and NLRC4. Cell Rep. 2017;21:3427-3444 pubmed publisher
Chang X, Wang L, Wang Z, Wu S, Zhu X, Hu S, et al. TRADD mediates the tumor necrosis factor-induced apoptosis of L929 cells in the absence of RIP3. Sci Rep. 2017;7:16111 pubmed publisher
Jiao Y, Li Z, Loughran P, Fan E, Scott M, Li Y, et al. Frontline Science: Macrophage-derived exosomes promote neutrophil necroptosis following hemorrhagic shock. J Leukoc Biol. 2017;: pubmed publisher
Mason A, Elia L, Finkbeiner S. The Receptor-interacting Serine/Threonine Protein Kinase 1 (RIPK1) Regulates Progranulin Levels. J Biol Chem. 2017;292:3262-3272 pubmed publisher
Perimenis P, Galaris A, Voulgari A, Prassa M, Pintzas A. IAP antagonists Birinapant and AT-406 efficiently synergise with either TRAIL, BRAF, or BCL-2 inhibitors to sensitise BRAFV600E colorectal tumour cells to apoptosis. BMC Cancer. 2016;16:624 pubmed publisher
Etemadi N, Chopin M, Anderton H, Tanzer M, Rickard J, Abeysekera W, et al. TRAF2 regulates TNF and NF-κB signalling to suppress apoptosis and skin inflammation independently of Sphingosine kinase 1. elife. 2015;4: pubmed publisher
Wang Y, Wang J, Huang Y, Liu F, Zeng W, Li Y, et al. Tissue acidosis induces neuronal necroptosis via ASIC1a channel independent of its ionic conduction. elife. 2015;4: pubmed publisher
Echeverry N, Ziltener G, Barbone D, Weder W, Stahel R, Broaddus V, et al. Inhibition of autophagy sensitizes malignant pleural mesothelioma cells to dual PI3K/mTOR inhibitors. Cell Death Dis. 2015;6:e1757 pubmed publisher
Cieply B, Koontz C, Frisch S. CD44S-hyaluronan interactions protect cells resulting from EMT against anoikis. Matrix Biol. 2015;48:55-65 pubmed publisher
Rickard J, Anderton H, Etemadi N, Nachbur U, Darding M, Peltzer N, et al. TNFR1-dependent cell death drives inflammation in Sharpin-deficient mice. elife. 2014;3: pubmed publisher
Zheng H, Chen J, Sha S. Receptor-interacting protein kinases modulate noise-induced sensory hair cell death. Cell Death Dis. 2014;5:e1262 pubmed publisher
Maas C, Tromp J, van Laar J, Thijssen R, Elias J, Malara A, et al. CLL cells are resistant to smac mimetics because of an inability to form a ripoptosome complex. Cell Death Dis. 2013;4:e782 pubmed publisher
Sosna J, Voigt S, Mathieu S, Lange A, Thon L, Davarnia P, et al. TNF-induced necroptosis and PARP-1-mediated necrosis represent distinct routes to programmed necrotic cell death. Cell Mol Life Sci. 2014;71:331-48 pubmed publisher
Moujalled D, Cook W, Okamoto T, Murphy J, Lawlor K, Vince J, et al. TNF can activate RIPK3 and cause programmed necrosis in the absence of RIPK1. Cell Death Dis. 2013;4:e465 pubmed publisher
Dunai Z, Imre G, Barna G, Korcsmáros T, Petak I, Bauer P, et al. Staurosporine induces necroptotic cell death under caspase-compromised conditions in U937 cells. PLoS ONE. 2012;7:e41945 pubmed publisher
Huang Y, Chen L, Zhou Y, Liu H, Yang J, Liu Z, et al. UXT-V1 protects cells against TNF-induced apoptosis through modulating complex II formation. Mol Biol Cell. 2011;22:1389-97 pubmed publisher
product information
Cat.# :
610458
Brand :
BD Transduction Laboratories
Name :
RIP
Format :
Purified
Clone :
38/RIP
Isotype :
Ms IgG 2a
React :
Hu , Ms , Rat , Dog , Chick
Conc :
250 µg/ml
Apps :
IF , IP , WB
Size :
50 µg
Status :
RUO
List Price :
207.00 USD
more info or order :
company information