product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
p27 Kip1
catalog :
610242
quantity :
150 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
57/Kip1/p27
reactivity :
human, mouse, rat, dogs
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation
more info or order :
citations: 48
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; fig 2
Orlando S, Gallastegui E, Besson A, Abril G, Aligué R, Pujol M, et al. p27Kip1 and p21Cip1 collaborate in the regulation of transcription by recruiting cyclin-Cdk complexes on the promoters of target genes. Nucleic Acids Res. 2015;43:6860-73 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...
Gualtieri A, Kyprianou N, Gregory L, Vignola M, Nicholson J, Tan R, et al. Activating mutations in BRAF disrupt the hypothalamo-pituitary axis leading to hypopituitarism in mice and humans. Nat Commun. 2021;12:2028 pubmed publisher
  • western blot; mouse; loading ...; fig 3d
Lee J, Park I, Kwak M, Rhee W, Kim S, Shin J. HMGB1 orchestrates STING-mediated senescence via TRIM30α modulation in cancer cells. Cell Death Discov. 2021;7:28 pubmed publisher
  • flow cytometry; human; 1:250; loading ...; fig s5d
Zatulovskiy E, Zhang S, Berenson D, Topacio B, Skotheim J. Cell growth dilutes the cell cycle inhibitor Rb to trigger cell division. Science. 2020;369:466-471 pubmed publisher
  • immunohistochemistry; human; fig 2g
  • western blot; human; loading ...; fig 2e
Sin Chan P, Mumal I, Suwal T, Ho B, Fan X, Singh I, et al. A C19MC-LIN28A-MYCN Oncogenic Circuit Driven by Hijacked Super-enhancers Is a Distinct Therapeutic Vulnerability in ETMRs: A Lethal Brain Tumor. Cancer Cell. 2019;36:51-67.e7 pubmed publisher
  • western blot; human; loading ...; fig 1d
Ishikawa Y, Gamo K, Yabuki M, Takagi S, Toyoshima K, Nakayama K, et al. A Novel LSD1 Inhibitor T-3775440 Disrupts GFI1B-Containing Complex Leading to Transdifferentiation and Impaired Growth of AML Cells. Mol Cancer Ther. 2017;16:273-284 pubmed publisher
  • western blot; human; fig 2h
Cramer S, Saha A, Liu J, Tadi S, Tiziani S, Yan W, et al. Systemic depletion of L-cyst(e)ine with cyst(e)inase increases reactive oxygen species and suppresses tumor growth. Nat Med. 2017;23:120-127 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...
Brocks T, Fedorchenko O, Schliermann N, Stein A, Moll U, Seegobin S, et al. Macrophage migration inhibitory factor protects from nonmelanoma epidermal tumors by regulating the number of antigen-presenting cells in skin. FASEB J. 2017;31:526-543 pubmed publisher
  • western blot; human; 0.5 ug/ml; loading ...; fig 3B
Uematsu K, Okumura F, Tonogai S, Joo Okumura A, Alemayehu D, Nishikimi A, et al. ASB7 regulates spindle dynamics and genome integrity by targeting DDA3 for proteasomal degradation. J Cell Biol. 2016;215:95-106 pubmed
  • western blot; mouse; loading ...; fig 2k
Jayapal S, Ang H, Wang C, Bisteau X, Caldez M, Xuan G, et al. Cyclin A2 regulates erythrocyte morphology and numbers. Cell Cycle. 2016;15:3070-3081 pubmed
  • western blot; human; fig 5
Ferrán B, Martí Pàmies I, Alonso J, Rodríguez Calvo R, Aguiló S, Vidal F, et al. The nuclear receptor NOR-1 regulates the small muscle protein, X-linked (SMPX) and myotube differentiation. Sci Rep. 2016;6:25944 pubmed publisher
  • western blot; human; fig 5
Cao C, Wang Z, Huang L, Bai L, Wang Y, Liang Y, et al. Down-regulation of tumor endothelial marker 8 suppresses cell proliferation mediated by ERK1/2 activity. Sci Rep. 2016;6:23419 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6e
Zhao H, Wang H, Bauzon F, Lu Z, Fu H, Cui J, et al. Deletions of Retinoblastoma 1 (Rb1) and Its Repressing Target S Phase Kinase-associated protein 2 (Skp2) Are Synthetic Lethal in Mouse Embryogenesis. J Biol Chem. 2016;291:10201-9 pubmed publisher
  • western blot; human; 1:1000; fig 1
Pavlides S, Lecanda J, Daubriac J, Pandya U, Gama P, Blank S, et al. TGF-β activates APC through Cdh1 binding for Cks1 and Skp2 proteasomal destruction stabilizing p27kip1 for normal endometrial growth. Cell Cycle. 2016;15:931-47 pubmed publisher
  • western blot; human; fig 6
Ardini E, Menichincheri M, Banfi P, Bosotti R, De Ponti C, Pulci R, et al. Entrectinib, a Pan-TRK, ROS1, and ALK Inhibitor with Activity in Multiple Molecularly Defined Cancer Indications. Mol Cancer Ther. 2016;15:628-39 pubmed publisher
  • western blot; human; fig 1
Podmirseg S, Jäkel H, Ranches G, Kullmann M, Sohm B, Villunger A, et al. Caspases uncouple p27(Kip1) from cell cycle regulated degradation and abolish its ability to stimulate cell migration and invasion. Oncogene. 2016;35:4580-90 pubmed publisher
  • western blot; human
Bornstein G, Grossman C. COP9-Signalosome deneddylase activity is enhanced by simultaneous neddylation: insights into the regulation of an enzymatic protein complex. Cell Div. 2015;10:5 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; fig 1
Zuckermann M, Hovestadt V, Knobbe Thomsen C, Zapatka M, Northcott P, Schramm K, et al. Somatic CRISPR/Cas9-mediated tumour suppressor disruption enables versatile brain tumour modelling. Nat Commun. 2015;6:7391 pubmed publisher
  • western blot; human
Oliveira C, de Bock C, Molloy T, Sadeqzadeh E, Geng X, Hersey P, et al. Macrophage migration inhibitory factor engages PI3K/Akt signalling and is a prognostic factor in metastatic melanoma. BMC Cancer. 2014;14:630 pubmed publisher
  • western blot; mouse
Lecona E, Rojas L, Bonasio R, Johnston A, Fernandez Capetillo O, Reinberg D. Polycomb protein SCML2 regulates the cell cycle by binding and modulating CDK/CYCLIN/p21 complexes. PLoS Biol. 2013;11:e1001737 pubmed publisher
  • western blot; mouse; 1:1000
Nakayama A, Nakayama M, Turner C, Höing S, Lepore J, Adams R. Ephrin-B2 controls PDGFR? internalization and signaling. Genes Dev. 2013;27:2576-89 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 0.5 ug/mL
Perez Sanz J, Arluzea J, Matorras R, González Santiago N, Bilbao J, Yeh N, et al. Increased number of multi-oocyte follicles (MOFs) in juvenile p27Kip1 mutant mice: potential role of granulosa cells. Hum Reprod. 2013;28:1023-30 pubmed publisher
  • western blot; mouse; fig 4
Diril M, Ratnacaram C, Padmakumar V, Du T, Wasser M, Coppola V, et al. Cyclin-dependent kinase 1 (Cdk1) is essential for cell division and suppression of DNA re-replication but not for liver regeneration. Proc Natl Acad Sci U S A. 2012;109:3826-31 pubmed publisher
Nowosad A, Creff J, Jeannot P, Culerrier R, Codogno P, Manenti S, et al. p27 controls autophagic vesicle trafficking in glucose-deprived cells via the regulation of ATAT1-mediated microtubule acetylation. Cell Death Dis. 2021;12:481 pubmed publisher
Costa C, Wang Y, Ly A, Hosono Y, Murchie E, Walmsley C, et al. PTEN Loss Mediates Clinical Cross-Resistance to CDK4/6 and PI3Kα Inhibitors in Breast Cancer. Cancer Discov. 2019;: pubmed publisher
Nachmani D, Bothmer A, Grisendi S, Mele A, Bothmer D, Lee J, et al. Germline NPM1 mutations lead to altered rRNA 2'-O-methylation and cause dyskeratosis congenita. Nat Genet. 2019;51:1518-1529 pubmed publisher
Arreal L, Piva M, Fernandez S, Revandkar A, Schaub Clerigué A, Villanueva J, et al. Targeting PML in triple negative breast cancer elicits growth suppression and senescence. Cell Death Differ. 2019;: pubmed publisher
Yin Y, Xu L, Chang Y, Zeng T, Chen X, Wang A, et al. N-Myc promotes therapeutic resistance development of neuroendocrine prostate cancer by differentially regulating miR-421/ATM pathway. Mol Cancer. 2019;18:11 pubmed publisher
Scott D, Hammill J, Min J, Rhee D, Connelly M, Sviderskiy V, et al. Blocking an N-terminal acetylation-dependent protein interaction inhibits an E3 ligase. Nat Chem Biol. 2017;13:850-857 pubmed publisher
Jeannot P, Nowosad A, Perchey R, Callot C, Bennana E, Katsube T, et al. p27Kip1 promotes invadopodia turnover and invasion through the regulation of the PAK1/Cortactin pathway. elife. 2017;6: pubmed publisher
Perearnau A, Orlando S, Islam A, Gallastegui E, Martinez J, Jordan A, et al. p27Kip1, PCAF and PAX5 cooperate in the transcriptional regulation of specific target genes. Nucleic Acids Res. 2017;45:5086-5099 pubmed publisher
Li L, Grausam K, Wang J, Lun M, Ohli J, Lidov H, et al. Sonic Hedgehog promotes proliferation of Notch-dependent monociliated choroid plexus tumour cells. Nat Cell Biol. 2016;18:418-30 pubmed publisher
Veas Pérez de Tudela M, Maestre C, Delgado Esteban M, Bolaños J, Almeida A. Cdk5-mediated inhibition of APC/C-Cdh1 switches on the cyclin D1-Cdk4-pRb pathway causing aberrant S-phase entry of postmitotic neurons. Sci Rep. 2015;5:18180 pubmed publisher
Rogers S, Gloss B, Lee C, Sergio C, Dinger M, Musgrove E, et al. Cyclin E2 is the predominant E-cyclin associated with NPAT in breast cancer cells. Cell Div. 2015;10:1 pubmed publisher
Vosper J, Masuccio A, Kullmann M, Ploner C, Geley S, Hengst L. Statin-induced depletion of geranylgeranyl pyrophosphate inhibits cell proliferation by a novel pathway of Skp2 degradation. Oncotarget. 2015;6:2889-902 pubmed
Gonzalez Granado J, Navarro Puche A, Molina Sánchez P, Blanco Berrocal M, Viana R, Font de Mora J, et al. Sorting nexin 6 enhances lamin a synthesis and incorporation into the nuclear envelope. PLoS ONE. 2014;9:e115571 pubmed publisher
Zindy F, Kawauchi D, Lee Y, Ayrault O, Ben Merzoug L, McKinnon P, et al. Role of the miR-17?92 cluster family in cerebellar and medulloblastoma development. Biol Open. 2014;3:597-605 pubmed publisher
Bjerke G, Pietrzak K, Melhuish T, Frierson H, Paschal B, Wotton D. Prostate cancer induced by loss of Apc is restrained by TGF? signaling. PLoS ONE. 2014;9:e92800 pubmed publisher
Falkenberg N, Anastasov N, Rappl K, Braselmann H, Auer G, Walch A, et al. MiR-221/-222 differentiate prognostic groups in advanced breast cancers and influence cell invasion. Br J Cancer. 2013;109:2714-23 pubmed publisher
Bjerke G, Yang C, Frierson H, Paschal B, Wotton D. Activation of Akt signaling in prostate induces a TGF?-mediated restraint on cancer progression and metastasis. Oncogene. 2014;33:3660-7 pubmed publisher
Grey W, Izatt L, Sahraoui W, Ng Y, Ogilvie C, Hulse A, et al. Deficiency of the cyclin-dependent kinase inhibitor, CDKN1B, results in overgrowth and neurodevelopmental delay. Hum Mutat. 2013;34:864-8 pubmed publisher
Chen L, Chen D, Kurtyka C, Rawal B, Fulp W, Haura E, et al. Tripartite motif containing 28 (Trim28) can regulate cell proliferation by bridging HDAC1/E2F interactions. J Biol Chem. 2012;287:40106-18 pubmed publisher
Wang H, Xu Y, Fang Z, Chen S, Balk S, Yuan X. Doxycycline regulated induction of AKT in murine prostate drives proliferation independently of p27 cyclin dependent kinase inhibitor downregulation. PLoS ONE. 2012;7:e41330 pubmed publisher
Pérez Luna M, Aguasca M, Perearnau A, Serratosa J, Martínez Balbás M, Jesús Pujol M, et al. PCAF regulates the stability of the transcriptional regulator and cyclin-dependent kinase inhibitor p27 Kip1. Nucleic Acids Res. 2012;40:6520-33 pubmed publisher
Fussbroich B, Wagener N, Macher Goeppinger S, Benner A, Fälth M, Sultmann H, et al. EZH2 depletion blocks the proliferation of colon cancer cells. PLoS ONE. 2011;6:e21651 pubmed publisher
Morishita D, Takami M, Yoshikawa S, Katayama R, Sato S, Kukimoto Niino M, et al. Cell-permeable carboxyl-terminal p27(Kip1) peptide exhibits anti-tumor activity by inhibiting Pim-1 kinase. J Biol Chem. 2011;286:2681-8 pubmed publisher
Kikuchi J, Kinoshita I, Shimizu Y, Oizumi S, Nishimura M, Birrer M, et al. Simultaneous blockade of AP-1 and phosphatidylinositol 3-kinase pathway in non-small cell lung cancer cells. Br J Cancer. 2008;99:2013-9 pubmed publisher
Trotman L, Niki M, Dotan Z, Koutcher J, Di Cristofano A, Xiao A, et al. Pten dose dictates cancer progression in the prostate. PLoS Biol. 2003;1:E59 pubmed
product information
Cat.# :
610242
Brand :
BD Transduction Laboratories
Name :
p27 Kip1
Format :
Purified
Clone :
57/Kip1/p27
Isotype :
Ms IgG 1
React :
Hu , Ms , Rat , Dog , Chick , Frog
Conc :
250 µg/ml
Apps :
IF , IHC , IP , WB
Size :
150 µg
Status :
RUO
more info or order :
company information