product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
p27 Kip1
catalog :
610241
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
57/Kip1/p27
reactivity :
human, mouse, rat, dogs, chicken
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation
more info or order :
citations: 67
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; rat; 1:1000; loading ...; fig s1d
Ding L, Shunkwiler L, Harper N, Zhao Y, Hinohara K, Huh S, et al. Deletion of Cdkn1b in ACI rats leads to increased proliferation and pregnancy-associated changes in the mammary gland due to perturbed systemic endocrine environment. PLoS Genet. 2019;15:e1008002 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig s4c
Walter D, Yates T, Ruiz Torres M, Kim Kiselak C, Gudiel A, Deshpande C, et al. RB constrains lineage fidelity and multiple stages of tumour progression and metastasis. Nature. 2019;569:423-427 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig 3b
Lawton A, Engstrom T, Rohrbach D, Omura M, Turnbull D, Mamou J, et al. Cerebellar folding is initiated by mechanical constraints on a fluid-like layer without a cellular pre-pattern. elife. 2019;8: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 2c
Chen X, Chanda A, Ikeuchi Y, Zhang X, Goodman J, Reddy N, et al. The Transcriptional Regulator SnoN Promotes the Proliferation of Cerebellar Granule Neuron Precursors in the Postnatal Mouse Brain. J Neurosci. 2019;39:44-62 pubmed publisher
  • western blot; human; loading ...; fig 2c
Juhasz A, Markel S, Gaur S, Liu H, Lu J, Jiang G, et al. NADPH oxidase 1 supports proliferation of colon cancer cells by modulating reactive oxygen species-dependent signal transduction. J Biol Chem. 2017;292:7866-7887 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 1d
  • immunocytochemistry; mouse; 1:500; fig s8b
Feng W, Kawauchi D, Körkel Qu H, Deng H, Serger E, Sieber L, et al. Chd7 is indispensable for mammalian brain development through activation of a neuronal differentiation programme. Nat Commun. 2017;8:14758 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...
Brocks T, Fedorchenko O, Schliermann N, Stein A, Moll U, Seegobin S, et al. Macrophage migration inhibitory factor protects from nonmelanoma epidermal tumors by regulating the number of antigen-presenting cells in skin. FASEB J. 2017;31:526-543 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 9a
Fujimura K, Mitsuhashi T, Shibata S, Shimozato S, Takahashi T. In Utero Exposure to Valproic Acid Induces Neocortical Dysgenesis via Dysregulation of Neural Progenitor Cell Proliferation/Differentiation. J Neurosci. 2016;36:10908-10919 pubmed
  • western blot; human; 1:1000; loading ...; fig 3d
Qi D, Cobrinik D. MDM2 but not MDM4 promotes retinoblastoma cell proliferation through p53-independent regulation of MYCN translation. Oncogene. 2017;36:1760-1769 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 2c
Xue C, Yu D, Gherardi S, Koach J, Milazzo G, Gamble L, et al. MYCN promotes neuroblastoma malignancy by establishing a regulatory circuit with transcription factor AP4. Oncotarget. 2016;7:54937-54951 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:1000; tbl 3
Frohwitter G, Buerger H, van Diest P, Korsching E, Kleinheinz J, Fillies T. Cytokeratin and protein expression patterns in squamous cell carcinoma of the oral cavity provide evidence for two distinct pathogenetic pathways. Oncol Lett. 2016;12:107-113 pubmed
  • western blot; human; fig S1B
Ding X, Jiang W, Zhou P, Liu L, Wan X, Yuan X, et al. Mixed Lineage Leukemia 5 (MLL5) Protein Stability Is Cooperatively Regulated by O-GlcNac Transferase (OGT) and Ubiquitin Specific Protease 7 (USP7). PLoS ONE. 2015;10:e0145023 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 1
  • western blot; mouse; 1:2000; fig 1
  • western blot; human; 1:2000; fig 4
Lyu L, Whitcomb E, Jiang S, Chang M, Gu Y, Duncan M, et al. Unfolded-protein response-associated stabilization of p27(Cdkn1b) interferes with lens fiber cell denucleation, leading to cataract. FASEB J. 2016;30:1087-95 pubmed publisher
  • western blot; human; 1:1000; fig 1
Qin J, Zhou Z, Chen W, Wang C, Zhang H, Ge G, et al. BAP1 promotes breast cancer cell proliferation and metastasis by deubiquitinating KLF5. Nat Commun. 2015;6:8471 pubmed publisher
  • western blot; human; 1:500; fig 1
Ge F, Chen W, Qin J, Zhou Z, Liu R, Liu L, et al. Ataxin-3 like (ATXN3L), a member of the Josephin family of deubiquitinating enzymes, promotes breast cancer proliferation by deubiquitinating Krüppel-like factor 5 (KLF5). Oncotarget. 2015;6:21369-78 pubmed
  • western blot; human; fig 1
Wang C, Nie Z, Zhou Z, Zhang H, Liu R, Wu J, et al. The interplay between TEAD4 and KLF5 promotes breast cancer partially through inhibiting the transcription of p27Kip1. Oncotarget. 2015;6:17685-97 pubmed
  • western blot; human; 1:1000
Xu D, Li C, Zhang X, Gong Z, Chan C, Lee S, et al. Skp2-macroH2A1-CDK8 axis orchestrates G2/M transition and tumorigenesis. Nat Commun. 2015;6:6641 pubmed publisher
  • western blot; human; fig 2
Vogel C, Smit M, Maddalo G, Possik P, Sparidans R, van der Burg S, et al. Cooperative induction of apoptosis in NRAS mutant melanoma by inhibition of MEK and ROCK. Pigment Cell Melanoma Res. 2015;28:307-17 pubmed publisher
  • immunohistochemistry; mouse; 1:300
Urness L, Wang X, Shibata S, Ohyama T, Mansour S. Fgf10 is required for specification of non-sensory regions of the cochlear epithelium. Dev Biol. 2015;400:59-71 pubmed publisher
  • western blot; mouse
Chen J, Shin J, Zhao R, Phan L, Wang H, Xue Y, et al. CSN6 drives carcinogenesis by positively regulating Myc stability. Nat Commun. 2014;5:5384 pubmed publisher
  • western blot; mouse
Perry M, Dufour C, Eichner L, Tsang D, Deblois G, Muller W, et al. ERBB2 deficiency alters an E2F-1-dependent adaptive stress response and leads to cardiac dysfunction. Mol Cell Biol. 2014;34:4232-43 pubmed publisher
  • western blot; mouse; 1:1000; fig 8
Nishimura Y, Shikanai M, Hoshino M, Ohshima T, Nabeshima Y, Mizutani K, et al. Cdk5 and its substrates, Dcx and p27kip1, regulate cytoplasmic dilation formation and nuclear elongation in migrating neurons. Development. 2014;141:3540-50 pubmed publisher
  • western blot; human
Wojdyla L, Stone A, Sethakorn N, Uppada S, Devito J, Bissonnette M, et al. T-oligo as an anticancer agent in colorectal cancer. Biochem Biophys Res Commun. 2014;446:596-601 pubmed publisher
  • western blot; human; 1:1000
Kim J, Kim H, Park J, Park D, Cho Y, Sohn C, et al. Epidermal growth factor upregulates Skp2/Cks1 and p27(kip1) in human extrahepatic cholangiocarcinoma cells. World J Gastroenterol. 2014;20:755-73 pubmed publisher
  • western blot; human
Fiori M, Barbini C, Haas T, Marroncelli N, Patrizii M, Biffoni M, et al. Antitumor effect of miR-197 targeting in p53 wild-type lung cancer. Cell Death Differ. 2014;21:774-82 pubmed publisher
  • western blot; mouse
van Boxtel R, Gomez Puerto C, Mokry M, Eijkelenboom A, van der Vos K, Nieuwenhuis E, et al. FOXP1 acts through a negative feedback loop to suppress FOXO-induced apoptosis. Cell Death Differ. 2013;20:1219-29 pubmed publisher
  • immunohistochemistry - frozen section; chicken; 1:200
Shirazi Fard S, Jarrin M, Boije H, Fillon V, All Eriksson C, Hallböök F. Heterogenic final cell cycle by chicken retinal Lim1 horizontal progenitor cells leads to heteroploid cells with a remaining replicated genome. PLoS ONE. 2013;8:e59133 pubmed publisher
  • western blot; human
Caldon C, Sergio C, Burgess A, Deans A, Sutherland R, Musgrove E. Cyclin E2 induces genomic instability by mechanisms distinct from cyclin E1. Cell Cycle. 2013;12:606-17 pubmed publisher
  • western blot; human; fig 7
Macia A, Gallel P, Vaquero M, Gou Fabregas M, Santacana M, Maliszewska A, et al. Sprouty1 is a candidate tumor-suppressor gene in medullary thyroid carcinoma. Oncogene. 2012;31:3961-72 pubmed publisher
  • western blot; mouse
Inaki M, Kato D, Utsugi T, Onoda F, Hanaoka F, Murakami Y. Genetic analyses using a mouse cell cycle mutant identifies magoh as a novel gene involved in Cdk regulation. Genes Cells. 2011;16:166-78 pubmed publisher
  • western blot; mouse; 1:500
Martín Ibáñez R, Crespo E, Urbán N, Sergent Tanguy S, Herranz C, Jaumot M, et al. Ikaros-1 couples cell cycle arrest of late striatal precursors with neurogenesis of enkephalinergic neurons. J Comp Neurol. 2010;518:329-51 pubmed publisher
  • western blot; human
Sahin O, Frohlich H, Löbke C, Korf U, Burmester S, Majety M, et al. Modeling ERBB receptor-regulated G1/S transition to find novel targets for de novo trastuzumab resistance. BMC Syst Biol. 2009;3:1 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100
Poche R, Furuta Y, Chaboissier M, Schedl A, Behringer R. Sox9 is expressed in mouse multipotent retinal progenitor cells and functions in Müller glial cell development. J Comp Neurol. 2008;510:237-50 pubmed publisher
  • immunohistochemistry; mouse; 1:200
Elshatory Y, Deng M, Xie X, Gan L. Expression of the LIM-homeodomain protein Isl1 in the developing and mature mouse retina. J Comp Neurol. 2007;503:182-97 pubmed
  • western blot; human
Chew E, Hagen T. Substrate-mediated regulation of cullin neddylation. J Biol Chem. 2007;282:17032-40 pubmed
Ng M, Gan Y, Hagen T. Characterisation of cellular effects of Burkholderia pseudomallei cycle inhibiting factor (Cif). Biol Open. 2018;7: pubmed publisher
Siddam A, Gautier Courteille C, Perez Campos L, Anand D, Kakrana A, Dang C, et al. The RNA-binding protein Celf1 post-transcriptionally regulates p27Kip1 and Dnase2b to control fiber cell nuclear degradation in lens development. PLoS Genet. 2018;14:e1007278 pubmed publisher
Mikamo M, Kitagawa K, Sakai S, Uchida C, Ohhata T, Nishimoto K, et al. Inhibiting Skp2 E3 Ligase Suppresses Bleomycin-Induced Pulmonary Fibrosis. Int J Mol Sci. 2018;19: pubmed publisher
Bohn J, Van Etten J, Schagat T, Bowman B, McEachin R, Freddolino P, et al. Identification of diverse target RNAs that are functionally regulated by human Pumilio proteins. Nucleic Acids Res. 2018;46:362-386 pubmed publisher
Grey W, Ivey A, Milne T, Haferlach T, Grimwade D, Uhlmann F, et al. The Cks1/Cks2 axis fine-tunes Mll1 expression and is crucial for MLL-rearranged leukaemia cell viability. Biochim Biophys Acta Mol Cell Res. 2018;1865:105-116 pubmed publisher
Srivastava S, Mohibi S, Mirza S, Band H, Band V. Epidermal Growth Factor Receptor activation promotes ADA3 acetylation through the AKT-p300 pathway. Cell Cycle. 2017;16:1515-1525 pubmed publisher
Iida Y, Ciechanover A, Marzese D, Hata K, Bustos M, Ono S, et al. Epigenetic Regulation of KPC1 Ubiquitin Ligase Affects the NF-?B Pathway in Melanoma. Clin Cancer Res. 2017;23:4831-4842 pubmed publisher
Griffin N, Sharma G, Zhao X, Mirza S, Srivastava S, Dave B, et al. ADA3 regulates normal and tumor mammary epithelial cell proliferation through c-MYC. Breast Cancer Res. 2016;18:113 pubmed
Gómez Puerto M, Verhagen L, Braat A, Lam E, Coffer P, Lorenowicz M. Activation of autophagy by FOXO3 regulates redox homeostasis during osteogenic differentiation. Autophagy. 2016;12:1804-1816 pubmed
Moura D, Fernández I, Marín Royo G, López Sánchez I, Martín Doncel E, Vega F, et al. Oncogenic Sox2 regulates and cooperates with VRK1 in cell cycle progression and differentiation. Sci Rep. 2016;6:28532 pubmed publisher
Li L, Grausam K, Wang J, Lun M, Ohli J, Lidov H, et al. Sonic Hedgehog promotes proliferation of Notch-dependent monociliated choroid plexus tumour cells. Nat Cell Biol. 2016;18:418-30 pubmed publisher
Hatori Y, Yan Y, Schmidt K, Furukawa E, Hasan N, Yang N, et al. Neuronal differentiation is associated with a redox-regulated increase of copper flow to the secretory pathway. Nat Commun. 2016;7:10640 pubmed publisher
Jiang Q, Cheng K, Mei X, Qiu J, Zhang W, Xue Y, et al. Synergistic anticancer effects of triptolide and celastrol, two main compounds from thunder god vine. Oncotarget. 2015;6:32790-804 pubmed publisher
Panopoulos A, Pacios Bras C, Choi J, Yenjerla M, Sussman M, Fotedar R, et al. Failure of cell cleavage induces senescence in tetraploid primary cells. Mol Biol Cell. 2014;25:3105-18 pubmed publisher
Vorpahl M, Schönhofer Merl S, Michaelis C, Flotho A, Melchior F, Wessely R. The Ran GTPase-activating protein (RanGAP1) is critically involved in smooth muscle cell differentiation, proliferation and migration following vascular injury: implications for neointima formation and restenosis. PLoS ONE. 2014;9:e101519 pubmed publisher
Federico A, Forzati F, Esposito F, Arra C, Palma G, Barbieri A, et al. Hmga1/Hmga2 double knock-out mice display a "superpygmy" phenotype. Biol Open. 2014;3:372-8 pubmed publisher
Zhang X, Fryknäs M, Hernlund E, Fayad W, De Milito A, Olofsson M, et al. Induction of mitochondrial dysfunction as a strategy for targeting tumour cells in metabolically compromised microenvironments. Nat Commun. 2014;5:3295 pubmed publisher
Cavalheiro G, Matos Rodrigues G, Gomes A, Rodrigues P, Martins R. c-Myc regulates cell proliferation during lens development. PLoS ONE. 2014;9:e87182 pubmed publisher
Vitale N, Kisslinger A, Paladino S, Procaccini C, Matarese G, Pierantoni G, et al. Resveratrol couples apoptosis with autophagy in UVB-irradiated HaCaT cells. PLoS ONE. 2013;8:e80728 pubmed publisher
Kullmann M, Grubbauer C, Goetsch K, Jakel H, Podmirseg S, Trockenbacher A, et al. The p27-Skp2 axis mediates glucocorticoid-induced cell cycle arrest in T-lymphoma cells. Cell Cycle. 2013;12:2625-35 pubmed publisher
Tan C, Hagen T. mTORC1 dependent regulation of REDD1 protein stability. PLoS ONE. 2013;8:e63970 pubmed publisher
Ring H, Mendu S, Shirazi Fard S, Birnir B, Hallböök F. GABA maintains the proliferation of progenitors in the developing chick ciliary marginal zone and non-pigmented ciliary epithelium. PLoS ONE. 2012;7:e36874 pubmed publisher
Loponen H, Ylikoski J, Albrecht J, Pirvola U. Restrictions in cell cycle progression of adult vestibular supporting cells in response to ectopic cyclin D1 expression. PLoS ONE. 2011;6:e27360 pubmed publisher
Basile K, Abel E, Aplin A. Adaptive upregulation of FOXD3 and resistance to PLX4032/4720-induced cell death in mutant B-RAF melanoma cells. Oncogene. 2012;31:2471-9 pubmed publisher
Chua Y, Boh B, Ponyeam W, Hagen T. Regulation of cullin RING E3 ubiquitin ligases by CAND1 in vivo. PLoS ONE. 2011;6:e16071 pubmed publisher
Rico Bautista E, Yang C, Lu L, Roth G, Wolf D. Chemical genetics approach to restoring p27Kip1 reveals novel compounds with antiproliferative activity in prostate cancer cells. BMC Biol. 2010;8:153 pubmed publisher
Xu B, Gerin I, Miao H, Vu Phan D, Johnson C, Xu R, et al. Multiple roles for the non-coding RNA SRA in regulation of adipogenesis and insulin sensitivity. PLoS ONE. 2010;5:e14199 pubmed publisher
Guo Z, Packard A, Krolewski R, Harris M, Manglapus G, Schwob J. Expression of pax6 and sox2 in adult olfactory epithelium. J Comp Neurol. 2010;518:4395-418 pubmed publisher
Pajalunga D, Puggioni E, Mazzola A, Leva V, Montecucco A, Crescenzi M. DNA replication is intrinsically hindered in terminally differentiated myotubes. PLoS ONE. 2010;5:e11559 pubmed publisher
Bossuyt W, Kazanjian A, De Geest N, Van Kelst S, De Hertogh G, Geboes K, et al. Atonal homolog 1 is a tumor suppressor gene. PLoS Biol. 2009;7:e39 pubmed publisher
Mercatelli N, Coppola V, Bonci D, Miele F, Costantini A, Guadagnoli M, et al. The inhibition of the highly expressed miR-221 and miR-222 impairs the growth of prostate carcinoma xenografts in mice. PLoS ONE. 2008;3:e4029 pubmed publisher
Musgrove E, Sergio C, Loi S, Inman C, Anderson L, Alles M, et al. Identification of functional networks of estrogen- and c-Myc-responsive genes and their relationship to response to tamoxifen therapy in breast cancer. PLoS ONE. 2008;3:e2987 pubmed publisher
product information
Cat.# :
610241
Brand :
BD Transduction Laboratories
Name :
p27 Kip1
Format :
Purified
Clone :
57/Kip1/p27
Isotype :
Ms IgG 1
React :
Hu , Ms , Rat , Dog , Chick , Frog
Conc :
250 µg/ml
Apps :
IF , IHC , IP , WB
Size :
50 µg
Status :
RUO
more info or order :
company information