product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
CD25
catalog :
564458
quantity :
25 µg
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
PC61
reactivity :
human, mouse
application :
immunocytochemistry, flow cytometry, blocking or activating experiments
more info or order :
citations: 70
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:200; loading ...; fig 1j
Goyette M, Elkholi I, Apcher C, Kuasne H, Rothlin C, Muller W, et al. Targeting Axl favors an antitumorigenic microenvironment that enhances immunotherapy responses by decreasing Hif-1α levels. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d
Kerselidou D, Dohai B, Nelson D, Daakour S, De Cock N, Hassoun Z, et al. Alternative glycosylation controls endoplasmic reticulum dynamics and tubular extension in mammalian cells. Sci Adv. 2021;7: pubmed publisher
  • flow cytometry; mouse; 1:100
Castiello M, Bosticardo M, Sacchetti N, Calzoni E, Fontana E, Yamazaki Y, et al. Efficacy and safety of anti-CD45-saporin as conditioning agent for RAG deficiency. J Allergy Clin Immunol. 2021;147:309-320.e6 pubmed publisher
  • flow cytometry; mouse; fig 2h
Williford J, Ishihara J, Ishihara A, Mansurov A, Hosseinchi P, Marchell T, et al. Recruitment of CD103+ dendritic cells via tumor-targeted chemokine delivery enhances efficacy of checkpoint inhibitor immunotherapy. Sci Adv. 2019;5:eaay1357 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b, 4b
Lecocq Q, Zeven K, De Vlaeminck Y, Martens S, Massa S, Goyvaerts C, et al. Noninvasive Imaging of the Immune Checkpoint LAG-3 Using Nanobodies, from Development to Pre-Clinical Use. Biomolecules. 2019;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Silva D, Yu S, Ulge U, Spangler J, Jude K, Labao Almeida C, et al. De novo design of potent and selective mimics of IL-2 and IL-15. Nature. 2019;565:186-191 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s8a
Dong S, Harrington B, Hu E, Greene J, Lehman A, Tran M, et al. PI3K p110δ inactivation antagonizes chronic lymphocytic leukemia and reverses T cell immune suppression. J Clin Invest. 2019;129:122-136 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3c, 4d
Atretkhany K, Mufazalov I, Dunst J, Kuchmiy A, Gogoleva V, Andruszewski D, et al. Intrinsic TNFR2 signaling in T regulatory cells provides protection in CNS autoimmunity. Proc Natl Acad Sci U S A. 2018;115:13051-13056 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s17a
Vendetti F, Karukonda P, Clump D, Teo T, Lalonde R, Nugent K, et al. ATR kinase inhibitor AZD6738 potentiates CD8+ T cell-dependent antitumor activity following radiation. J Clin Invest. 2018;128:3926-3940 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1g
Tanaka S, Pfleger C, Lai J, Roan F, Sun S, Ziegler S. KAP1 Regulates Regulatory T Cell Function and Proliferation in Both Foxp3-Dependent and -Independent Manners. Cell Rep. 2018;23:796-807 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 3a
Olesen M, Christiansen J, Petersen S, Jensen P, Paslawski W, Romero Ramos M, et al. CD4 T cells react to local increase of α-synuclein in a pathology-associated variant-dependent manner and modify brain microglia in absence of brain pathology. Heliyon. 2018;4:e00513 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4e
Seki A, Rutz S. Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells. J Exp Med. 2018;215:985-997 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Fahl S, Coffey F, Kain L, Zarin P, Dunbrack R, Teyton L, et al. Role of a selecting ligand in shaping the murine γδ-TCR repertoire. Proc Natl Acad Sci U S A. 2018;115:1889-1894 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1b
Szilagyi B, Triebus J, Kressler C, De Almeida M, Tierling S, Durek P, et al. Gut memories do not fade: epigenetic regulation of lasting gut homing receptor expression in CD4+ memory T cells. Mucosal Immunol. 2017;10:1443-1454 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2
Blanquiceth Y, Rodríguez Perea A, Tabares Guevara J, Correa L, Sánchez M, Ramirez Pineda J, et al. Increase of Frequency and Modulation of Phenotype of Regulatory T Cells by Atorvastatin Is Associated with Decreased Lung Inflammatory Cell Infiltration in a Murine Model of Acute Allergic Asthma. Front Immunol. 2016;7:620 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3c
Wei Y, Lu C, Chen J, Cui G, Wang L, Yu T, et al. High salt diet stimulates gut Th17 response and exacerbates TNBS-induced colitis in mice. Oncotarget. 2017;8:70-82 pubmed publisher
  • flow cytometry; mouse; 2:100; loading ...; fig 5f
Morita K, Okamura T, Inoue M, Komai T, Teruya S, Iwasaki Y, et al. Egr2 and Egr3 in regulatory T cells cooperatively control systemic autoimmunity through Ltbp3-mediated TGF-β3 production. Proc Natl Acad Sci U S A. 2016;113:E8131-E8140 pubmed
  • flow cytometry; mouse; loading ...; fig 2d
Jirmo A, Daluege K, Happle C, Albrecht M, Dittrich A, Busse M, et al. IL-27 Is Essential for Suppression of Experimental Allergic Asthma by the TLR7/8 Agonist R848 (Resiquimod). J Immunol. 2016;197:4219-4227 pubmed
  • flow cytometry; mouse; loading ...; fig s2b
Coursey T, Bian F, Zaheer M, Pflugfelder S, Volpe E, de Paiva C. Age-related spontaneous lacrimal keratoconjunctivitis is accompanied by dysfunctional T regulatory cells. Mucosal Immunol. 2017;10:743-756 pubmed publisher
  • flow cytometry; mouse; 1:200
Fabbiano S, Suárez Zamorano N, Rigo D, Veyrat Durebex C, Stevanovic Dokic A, Colin D, et al. Caloric Restriction Leads to Browning of White Adipose Tissue through Type 2 Immune Signaling. Cell Metab. 2016;24:434-446 pubmed publisher
  • flow cytometry; mouse; fig s2
Drake L, Iijima K, Bartemes K, Kita H. Group 2 Innate Lymphoid Cells Promote an Early Antibody Response to a Respiratory Antigen in Mice. J Immunol. 2016;197:1335-42 pubmed publisher
  • flow cytometry; mouse
Kathania M, Khare P, Zeng M, Cantarel B, Zhang H, Ueno H, et al. Itch inhibits IL-17-mediated colon inflammation and tumorigenesis by ROR-?t ubiquitination. Nat Immunol. 2016;17:997-1004 pubmed publisher
  • flow cytometry; mouse; fig s4
Brandstätter O, Schanz O, Vorac J, König J, Mori T, Maruyama T, et al. Balancing intestinal and systemic inflammation through cell type-specific expression of the aryl hydrocarbon receptor repressor. Sci Rep. 2016;6:26091 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3e
Rao E, Zhang Y, Li Q, Hao J, Egilmez N, Suttles J, et al. AMPK-dependent and independent effects of AICAR and compound C on T-cell responses. Oncotarget. 2016;7:33783-95 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3b
Salao K, Jiang L, Li H, Tsai V, Husaini Y, Curmi P, et al. CLIC1 regulates dendritic cell antigen processing and presentation by modulating phagosome acidification and proteolysis. Biol Open. 2016;5:620-30 pubmed publisher
  • flow cytometry; mouse; 1:200; fig 3
Swamy M, Pathak S, Grzes K, Damerow S, Sinclair L, van Aalten D, et al. Glucose and glutamine fuel protein O-GlcNAcylation to control T cell self-renewal and malignancy. Nat Immunol. 2016;17:712-20 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
Goldstein J, Burlion A, Zaragoza B, Sendeyo K, Polansky J, Huehn J, et al. Inhibition of the JAK/STAT Signaling Pathway in Regulatory T Cells Reveals a Very Dynamic Regulation of Foxp3 Expression. PLoS ONE. 2016;11:e0153682 pubmed publisher
  • immunocytochemistry; mouse
Riquelme S, Pogu J, Anegon I, Bueno S, Kalergis A. Carbon monoxide impairs mitochondria-dependent endosomal maturation and antigen presentation in dendritic cells. Eur J Immunol. 2015;45:3269-88 pubmed publisher
  • flow cytometry; mouse
Ladell K, Hazenberg M, Fitch M, Emson C, McEvoy Hein Asgarian B, Mold J, et al. Continuous Antigenic Stimulation of DO11.10 TCR Transgenic Mice in the Presence or Absence of IL-1?: Possible Implications for Mechanisms of T Cell Depletion in HIV Disease. J Immunol. 2015;195:4096-105 pubmed publisher
  • flow cytometry; mouse; loading ...
Martin Blondel G, Pignolet B, Tietz S, Yshii L, Gebauer C, Périnat T, et al. Migration of encephalitogenic CD8 T cells into the central nervous system is dependent on the α4β1-integrin. Eur J Immunol. 2015;45:3302-12 pubmed publisher
  • flow cytometry; mouse; fig 2
Fiume G, Scialdone A, Albano F, Rossi A, Tuccillo F, Rea D, et al. Impairment of T cell development and acute inflammatory response in HIV-1 Tat transgenic mice. Sci Rep. 2015;5:13864 pubmed publisher
  • flow cytometry; mouse; fig s3
Saulep Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, et al. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. Leukemia. 2016;30:163-72 pubmed publisher
  • flow cytometry; mouse
Ackerknecht M, Hauser M, Legler D, Stein J. In vivo TCR Signaling in CD4(+) T Cells Imprints a Cell-Intrinsic, Transient Low-Motility Pattern Independent of Chemokine Receptor Expression Levels, or Microtubular Network, Integrin, and Protein Kinase C Activity. Front Immunol. 2015;6:297 pubmed publisher
  • flow cytometry; mouse; fig 5
  • immunocytochemistry; mouse; fig 4
Thiault N, Darrigues J, Adoue V, Gros M, Binet B, Pérals C, et al. Peripheral regulatory T lymphocytes recirculating to the thymus suppress the development of their precursors. Nat Immunol. 2015;16:628-34 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4
Bouchery T, Kyle R, Camberis M, Shepherd A, Filbey K, Smith A, et al. ILC2s and T cells cooperate to ensure maintenance of M2 macrophages for lung immunity against hookworms. Nat Commun. 2015;6:6970 pubmed publisher
  • flow cytometry; mouse; fig 1
Charlton J, Tsoukatou D, Mamalaki C, Chatzidakis I. Programmed death 1 regulates memory phenotype CD4 T cell accumulation, inhibits expansion of the effector memory phenotype subset and modulates production of effector cytokines. PLoS ONE. 2015;10:e0119200 pubmed publisher
  • flow cytometry; mouse; fig 8
Van Den Ham K, Shio M, Rainone A, Fournier S, Krawczyk C, Olivier M. Iron prevents the development of experimental cerebral malaria by attenuating CXCR3-mediated T cell chemotaxis. PLoS ONE. 2015;10:e0118451 pubmed publisher
  • flow cytometry; mouse; fig 1
Michelet X, Garg S, Wolf B, Tuli A, Ricciardi Castagnoli P, Brenner M. MHC class II presentation is controlled by the lysosomal small GTPase, Arl8b. J Immunol. 2015;194:2079-88 pubmed publisher
  • flow cytometry; mouse; fig 1
Wirsdörfer F, Bangen J, Pastille E, Hansen W, Flohé S. Breaking the co-operation between bystander T-cells and natural killer cells prevents the development of immunosuppression after traumatic skeletal muscle injury in mice. Clin Sci (Lond). 2015;128:825-38 pubmed publisher
  • flow cytometry; mouse; fig 8
Zhang Y, Wu B, Metelli A, Thaxton J, Hong F, Rachidi S, et al. GP96 is a GARP chaperone and controls regulatory T cell functions. J Clin Invest. 2015;125:859-69 pubmed publisher
  • flow cytometry; mouse; 1.0 ug/ml; fig 3
Bergot A, Monnet N, Le Tran S, Mittal D, Al Kouba J, Steptoe R, et al. HPV16 E7 expression in skin induces TSLP secretion, type 2 ILC infiltration and atopic dermatitis-like lesions. Immunol Cell Biol. 2015;93:540-7 pubmed publisher
  • flow cytometry; human; fig 5
Hannani D, Vétizou M, Enot D, Rusakiewicz S, Chaput N, Klatzmann D, et al. Anticancer immunotherapy by CTLA-4 blockade: obligatory contribution of IL-2 receptors and negative prognostic impact of soluble CD25. Cell Res. 2015;25:208-24 pubmed publisher
  • flow cytometry; mouse; fig 4
Krishnamoorthy N, Burkett P, Dalli J, Abdulnour R, Colas R, Ramon S, et al. Cutting edge: maresin-1 engages regulatory T cells to limit type 2 innate lymphoid cell activation and promote resolution of lung inflammation. J Immunol. 2015;194:863-7 pubmed publisher
  • flow cytometry; mouse
Yuan X, Dee M, Altman N, Malek T. IL-2Rβ-dependent signaling and CD103 functionally cooperate to maintain tolerance in the gut mucosa. J Immunol. 2015;194:1334-46 pubmed publisher
  • flow cytometry; mouse; fig 5
Böhm L, Maxeiner J, Meyer Martin H, Reuter S, Finotto S, Klein M, et al. IL-10 and regulatory T cells cooperate in allergen-specific immunotherapy to ameliorate allergic asthma. J Immunol. 2015;194:887-97 pubmed publisher
  • flow cytometry; mouse
Rutz S, Kayagaki N, Phung Q, Eidenschenk C, Noubade R, Wang X, et al. Deubiquitinase DUBA is a post-translational brake on interleukin-17 production in T cells. Nature. 2015;518:417-21 pubmed publisher
  • flow cytometry; mouse; fig 2
Uchiyama M, Jin X, Yin E, Shimokawa T, Niimi M. Treadmill exercise induces murine cardiac allograft survival and generates regulatory T cell. Transpl Int. 2015;28:352-62 pubmed publisher
  • flow cytometry; mouse
Kern J, Drutel R, Leanhart S, Bogacz M, Pacholczyk R. Reduction of T cell receptor diversity in NOD mice prevents development of type 1 diabetes but not Sjögren's syndrome. PLoS ONE. 2014;9:e112467 pubmed publisher
  • flow cytometry; mouse; fig 2
Velardi E, Tsai J, Holland A, Wertheimer T, Yu V, Zakrzewski J, et al. Sex steroid blockade enhances thymopoiesis by modulating Notch signaling. J Exp Med. 2014;211:2341-9 pubmed publisher
  • flow cytometry; mouse
Chen M, Chen Y, Wu M, Yu G, Lin W, Tan T, et al. PP4 is essential for germinal center formation and class switch recombination in mice. PLoS ONE. 2014;9:e107505 pubmed publisher
  • flow cytometry; mouse; fig 5
Naik E, Webster J, DeVoss J, Liu J, Suriben R, Dixit V. Regulation of proximal T cell receptor signaling and tolerance induction by deubiquitinase Usp9X. J Exp Med. 2014;211:1947-55 pubmed publisher
  • flow cytometry; mouse
Penaloza MacMaster P, Kamphorst A, Wieland A, Araki K, Iyer S, West E, et al. Interplay between regulatory T cells and PD-1 in modulating T cell exhaustion and viral control during chronic LCMV infection. J Exp Med. 2014;211:1905-18 pubmed publisher
  • flow cytometry; mouse
Weber G, Chousterman B, Hilgendorf I, Robbins C, Theurl I, Gerhardt L, et al. Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis. J Exp Med. 2014;211:1243-56 pubmed publisher
  • flow cytometry; mouse
Zhang Y, Yan W, Mathew E, Bednar F, Wan S, Collins M, et al. CD4+ T lymphocyte ablation prevents pancreatic carcinogenesis in mice. Cancer Immunol Res. 2014;2:423-35 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 6c
León B, Bradley J, Lund F, Randall T, Ballesteros Tato A. FoxP3+ regulatory T cells promote influenza-specific Tfh responses by controlling IL-2 availability. Nat Commun. 2014;5:3495 pubmed publisher
  • flow cytometry; mouse
Joedicke J, Dietze K, Zelinskyy G, Dittmer U. The phenotype and activation status of regulatory T cells during Friend retrovirus infection. Virol Sin. 2014;29:48-60 pubmed publisher
  • flow cytometry; mouse
Hu Y, Xiao H, Shi T, Oppenheim J, Chen X. Progranulin promotes tumour necrosis factor-induced proliferation of suppressive mouse CD4? Foxp3? regulatory T cells. Immunology. 2014;142:193-201 pubmed publisher
  • flow cytometry; mouse
Saligrama N, Case L, Krementsov D, Teuscher C. Histamine H₂ receptor signaling × environment interactions determine susceptibility to experimental allergic encephalomyelitis. FASEB J. 2014;28:1898-909 pubmed publisher
  • flow cytometry; mouse
Kim E, Gasper D, Lee S, Plisch E, Svaren J, Suresh M. Bach2 regulates homeostasis of Foxp3+ regulatory T cells and protects against fatal lung disease in mice. J Immunol. 2014;192:985-95 pubmed publisher
  • flow cytometry; mouse
Chan I, Jain R, Tessmer M, Gorman D, Mangadu R, Sathe M, et al. Interleukin-23 is sufficient to induce rapid de novo gut tumorigenesis, independent of carcinogens, through activation of innate lymphoid cells. Mucosal Immunol. 2014;7:842-56 pubmed publisher
  • flow cytometry; mouse
Qiao G, Zhao Y, Li Z, Tang P, Langdon W, Yang T, et al. T cell activation threshold regulated by E3 ubiquitin ligase Cbl-b determines fate of inducible regulatory T cells. J Immunol. 2013;191:632-9 pubmed publisher
  • flow cytometry; mouse
Toker A, Engelbert D, Garg G, Polansky J, Floess S, Miyao T, et al. Active demethylation of the Foxp3 locus leads to the generation of stable regulatory T cells within the thymus. J Immunol. 2013;190:3180-8 pubmed publisher
  • flow cytometry; mouse; fig 2
Uchiyama M, Jin X, Zhang Q, Hirai T, Amano A, Bashuda H, et al. Auditory stimulation of opera music induced prolongation of murine cardiac allograft survival and maintained generation of regulatory CD4+CD25+ cells. J Cardiothorac Surg. 2012;7:26 pubmed publisher
  • blocking or activating experiments; mouse; fig 2
Oestreich K, Mohn S, Weinmann A. Molecular mechanisms that control the expression and activity of Bcl-6 in TH1 cells to regulate flexibility with a TFH-like gene profile. Nat Immunol. 2012;13:405-11 pubmed publisher
  • flow cytometry; mouse
Hu H, Wang B, Borde M, Nardone J, Maika S, Allred L, et al. Foxp1 is an essential transcriptional regulator of B cell development. Nat Immunol. 2006;7:819-26 pubmed
Dooms H, Wolslegel K, Lin P, Abbas A. Interleukin-2 enhances CD4+ T cell memory by promoting the generation of IL-7R alpha-expressing cells. J Exp Med. 2007;204:547-57 pubmed
Maillard I, Tu L, Sambandam A, Yashiro Ohtani Y, Millholland J, Keeshan K, et al. The requirement for Notch signaling at the beta-selection checkpoint in vivo is absolute and independent of the pre-T cell receptor. J Exp Med. 2006;203:2239-45 pubmed
Bettelli E, Sullivan B, Szabo S, Sobel R, Glimcher L, Kuchroo V. Loss of T-bet, but not STAT1, prevents the development of experimental autoimmune encephalomyelitis. J Exp Med. 2004;200:79-87 pubmed
Caramalho I, Lopes Carvalho T, Ostler D, Zelenay S, Haury M, Demengeot J. Regulatory T cells selectively express toll-like receptors and are activated by lipopolysaccharide. J Exp Med. 2003;197:403-11 pubmed
Piccirillo C, Letterio J, Thornton A, McHugh R, Mamura M, Mizuhara H, et al. CD4(+)CD25(+) regulatory T cells can mediate suppressor function in the absence of transforming growth factor beta1 production and responsiveness. J Exp Med. 2002;196:237-46 pubmed
product information
Cat.# :
564458
Brand :
BD Horizon
Name :
CD25
Format :
BB515
Clone :
PC61
Isotype :
Rat IgG 1
React :
Ms
Conc :
0.2 mg/ml
Apps :
FCM
Size :
25 µg
Status :
RUO
more info or order :
company information