product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
p21
catalog :
556431
quantity :
0.1 mg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
SXM30
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 65
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; loading ...
Goncalves S, Yin K, Ito Y, Chan A, Olan I, Gough S, et al. COX2 regulates senescence secretome composition and senescence surveillance through PGE2. Cell Rep. 2021;34:108860 pubmed publisher
  • western blot; mouse; loading ...; fig 1e
Lee J, Park I, Kwak M, Rhee W, Kim S, Shin J. HMGB1 orchestrates STING-mediated senescence via TRIM30α modulation in cancer cells. Cell Death Discov. 2021;7:28 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 2e
Rothmiller S, Jäger N, Meier N, Meyer T, Neu A, Steinritz D, et al. Chronic senescent human mesenchymal stem cells as possible contributor to the wound healing disorder after exposure to the alkylating agent sulfur mustard. Arch Toxicol. 2021;95:727-747 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; fig s1h
Morris J, Yashinskie J, Koche R, Chandwani R, Tian S, Chen C, et al. α-Ketoglutarate links p53 to cell fate during tumour suppression. Nature. 2019;573:595-599 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig 1d
McMahon M, Contreras A, Holm M, Uechi T, Forester C, Pang X, et al. A single H/ACA small nucleolar RNA mediates tumor suppression downstream of oncogenic RAS. elife. 2019;8: pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 1e
  • western blot; human; 1:1000; loading ...; fig 1g
Gal H, Lysenko M, Stroganov S, Vadai E, Youssef S, Tzadikevitch Geffen K, et al. Molecular pathways of senescence regulate placental structure and function. EMBO J. 2019;38:e100849 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig 6c, s2d
Hari P, Millar F, Tarrats N, Birch J, Quintanilla A, Rink C, et al. The innate immune sensor Toll-like receptor 2 controls the senescence-associated secretory phenotype. Sci Adv. 2019;5:eaaw0254 pubmed publisher
  • western blot; human; loading ...; fig s1a
Kucab J, Zou X, Morganella S, Joel M, Nanda A, Nagy E, et al. A Compendium of Mutational Signatures of Environmental Agents. Cell. 2019;177:821-836.e16 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 3i
Ritschka B, Storer M, Mas A, Heinzmann F, Ortells M, Morton J, et al. The senescence-associated secretory phenotype induces cellular plasticity and tissue regeneration. Genes Dev. 2017;31:172-183 pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig 2a
Kucab J, Hollstein M, Arlt V, Phillips D. Nutlin-3a selects for cells harbouring TP53 mutations. Int J Cancer. 2017;140:877-887 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 2c
Schlierf A, Altmann E, Quancard J, Jefferson A, Assenberg R, Renatus M, et al. Targeted inhibition of the COP9 signalosome for treatment of cancer. Nat Commun. 2016;7:13166 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 6c
Hoare M, Ito Y, Kang T, Weekes M, Matheson N, Patten D, et al. NOTCH1 mediates a switch between two distinct secretomes during senescence. Nat Cell Biol. 2016;18:979-92 pubmed publisher
  • immunohistochemistry; mouse; 1:10; loading ...; fig s1
Dutta A, Le Magnen C, Mitrofanova A, Ouyang X, Califano A, Abate Shen C. Identification of an NKX3.1-G9a-UTY transcriptional regulatory network that controls prostate differentiation. Science. 2016;352:1576-80 pubmed publisher
  • western blot; mouse; loading ...; fig 1e
Chesnokova V, Zonis S, Zhou C, Recouvreux M, Ben Shlomo A, Araki T, et al. Growth hormone is permissive for neoplastic colon growth. Proc Natl Acad Sci U S A. 2016;113:E3250-9 pubmed publisher
  • western blot; human; fig 1
  • western blot; mouse; fig 1
Yosef R, Pilpel N, Tokarsky Amiel R, Biran A, Ovadya Y, Cohen S, et al. Directed elimination of senescent cells by inhibition of BCL-W and BCL-XL. Nat Commun. 2016;7:11190 pubmed publisher
  • western blot; human; fig 6
Ardini E, Menichincheri M, Banfi P, Bosotti R, De Ponti C, Pulci R, et al. Entrectinib, a Pan-TRK, ROS1, and ALK Inhibitor with Activity in Multiple Molecularly Defined Cancer Indications. Mol Cancer Ther. 2016;15:628-39 pubmed publisher
  • western blot; mouse; 1:2000; fig s4
Kucab J, Zwart E, van Steeg H, Luijten M, Schmeiser H, Phillips D, et al. TP53 and lacZ mutagenesis induced by 3-nitrobenzanthrone in Xpa-deficient human TP53 knock-in mouse embryo fibroblasts. DNA Repair (Amst). 2016;39:21-33 pubmed publisher
  • western blot; human; fig 11
Daou S, Hammond Martel I, Mashtalir N, Barbour H, Gagnon J, Iannantuono N, et al. The BAP1/ASXL2 Histone H2A Deubiquitinase Complex Regulates Cell Proliferation and Is Disrupted in Cancer. J Biol Chem. 2015;290:28643-63 pubmed publisher
  • western blot; human; fig 3d
Wang J, Hua W, Huang S, Fan K, Takeshima L, Mao Y, et al. RASSF8 regulates progression of cutaneous melanoma through nuclear factor-κb. Oncotarget. 2015;6:30165-77 pubmed publisher
  • western blot; human; 1:500
Trokovic R, Weltner J, Noisa P, Raivio T, Otonkoski T. Combined negative effect of donor age and time in culture on the reprogramming efficiency into induced pluripotent stem cells. Stem Cell Res. 2015;15:254-62 pubmed publisher
  • western blot; human; 1:1000; fig 1
Moiseeva O, Lessard F, Acevedo Aquino M, Vernier M, Tsantrizos Y, Ferbeyre G. Mutant lamin A links prophase to a p53 independent senescence program. Cell Cycle. 2015;14:2408-21 pubmed publisher
  • western blot; human
Scala F, Brighenti E, Govoni M, Imbrogno E, Fornari F, Treré D, et al. Direct relationship between the level of p53 stabilization induced by rRNA synthesis-inhibiting drugs and the cell ribosome biogenesis rate. Oncogene. 2016;35:977-89 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 1
Gergics P, Brinkmeier M, Camper S. Lhx4 deficiency: increased cyclin-dependent kinase inhibitor expression and pituitary hypoplasia. Mol Endocrinol. 2015;29:597-612 pubmed publisher
  • western blot; mouse
Cai K, Wang Y, Smith E, Smedberg J, Yang D, Yang W, et al. Global deletion of Trp53 reverts ovarian tumor phenotype of the germ cell-deficient white spotting variant (Wv) mice. Neoplasia. 2015;17:89-100 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...
Raouf S, Weston C, Yucel N. Registered report: senescence surveillance of pre-malignant hepatocytes limits liver cancer development. elife. 2015;4: pubmed publisher
  • western blot; human; 1:2000; fig 1
Wohak L, Krais A, Kucab J, Stertmann J, Øvrebø S, Seidel A, et al. Carcinogenic polycyclic aromatic hydrocarbons induce CYP1A1 in human cells via a p53-dependent mechanism. Arch Toxicol. 2016;90:291-304 pubmed publisher
  • western blot; human
Mungamuri S, Wang S, Manfredi J, Gu W, Aaronson S. Ash2L enables P53-dependent apoptosis by favoring stable transcription pre-initiation complex formation on its pro-apoptotic target promoters. Oncogene. 2015;34:2461-70 pubmed publisher
  • western blot; human
Ramdzan Z, Vadnais C, Pal R, Vandal G, Cadieux C, Leduy L, et al. RAS transformation requires CUX1-dependent repair of oxidative DNA damage. PLoS Biol. 2014;12:e1001807 pubmed publisher
  • western blot; mouse; fig 6a
Berkenkamp B, Susnik N, Baisantry A, Kuznetsova I, Jacobi C, Sörensen Zender I, et al. In vivo and in vitro analysis of age-associated changes and somatic cellular senescence in renal epithelial cells. PLoS ONE. 2014;9:e88071 pubmed publisher
  • western blot; mouse
Parsons M, McCormick L, Janke L, Howard A, Bouchier Hayes L, Green D. Genetic deletion of caspase-2 accelerates MMTV/c-neu-driven mammary carcinogenesis in mice. Cell Death Differ. 2013;20:1174-82 pubmed publisher
  • western blot; human
Strauss G, Westhoff M, Fischer Posovszky P, Fulda S, Schanbacher M, Eckhoff S, et al. 4-hydroperoxy-cyclophosphamide mediates caspase-independent T-cell apoptosis involving oxidative stress-induced nuclear relocation of mitochondrial apoptogenic factors AIF and EndoG. Cell Death Differ. 2008;15:332-43 pubmed
Chrysanthou S, Flores J, Dawlaty M. Tet1 Suppresses p21 to Ensure Proper Cell Cycle Progression in Embryonic Stem Cells. Cells. 2022;11: pubmed publisher
Jeffery D, Gatto A, Podsypanina K, Renaud Pageot C, Ponce Landete R, Bonneville L, et al. CENP-A overexpression promotes distinct fates in human cells, depending on p53 status. Commun Biol. 2021;4:417 pubmed publisher
Haider N, Dutt P, van de Kooij B, Ho J, Palomero L, Pujana M, et al. NEK10 tyrosine phosphorylates p53 and controls its transcriptional activity. Oncogene. 2020;39:5252-5266 pubmed publisher
Majumder M, PALANISAMY V. RNA binding protein FXR1-miR301a-3p axis contributes to p21WAF1 degradation in oral cancer. PLoS Genet. 2020;16:e1008580 pubmed publisher
Burocziova M, Burdova K, Martinikova A, Kaspárek P, Kleiblova P, Danielsen S, et al. Truncated PPM1D impairs stem cell response to genotoxic stress and promotes growth of APC-deficient tumors in the mouse colon. Cell Death Dis. 2019;10:818 pubmed publisher
Wohak L, Baranski A, Krais A, Schmeiser H, Phillips D, Arlt V. The impact of p53 function on the metabolic activation of the carcinogenic air pollutant 3-nitrobenzanthrone and its metabolites 3-aminobenzanthrone and N-hydroxy-3-aminobenzanthrone in human cells. Mutagenesis. 2018;33:311-321 pubmed publisher
Chrysanthou S, Senner C, Woods L, Fineberg E, Okkenhaug H, Burge S, et al. A Critical Role of TET1/2 Proteins in Cell-Cycle Progression of Trophoblast Stem Cells. Stem Cell Reports. 2018;10:1355-1368 pubmed publisher
Vecino R, Burguete M, Jover Mengual T, Agulla J, Bobo Jiménez V, Salom J, et al. The MDM2-p53 pathway is involved in preconditioning-induced neuronal tolerance to ischemia. Sci Rep. 2018;8:1610 pubmed publisher
Glück S, Guey B, Gulen M, Wolter K, Kang T, Schmacke N, et al. Innate immune sensing of cytosolic chromatin fragments through cGAS promotes senescence. Nat Cell Biol. 2017;19:1061-1070 pubmed publisher
Du W, Hu J, Du W, Klein O. Lineage tracing of epithelial cells in developing teeth reveals two strategies for building signaling centers. J Biol Chem. 2017;292:15062-15069 pubmed publisher
Yosef R, Pilpel N, Papismadov N, Gal H, Ovadya Y, Vadai E, et al. p21 maintains senescent cell viability under persistent DNA damage response by restraining JNK and caspase signaling. EMBO J. 2017;36:2280-2295 pubmed publisher
Lin H, Simon P, Ysla R, Zeichner S, Brewer G, Rabson A. RNA stability regulates human T cell leukemia virus type 1 gene expression in chronically-infected CD4 T cells. Virology. 2017;508:7-17 pubmed publisher
Hoppe Seyler K, Bossler F, Lohrey C, Bulkescher J, Rösl F, Jansen L, et al. Induction of dormancy in hypoxic human papillomavirus-positive cancer cells. Proc Natl Acad Sci U S A. 2017;114:E990-E998 pubmed publisher
Münst B, Thier M, Winnemöller D, Helfen M, Thummer R, Edenhofer F. Nanog induces suppression of senescence through downregulation of p27KIP1 expression. J Cell Sci. 2016;129:912-20 pubmed publisher
Morrissy A, Garzia L, Shih D, Zuyderduyn S, Huang X, Skowron P, et al. Divergent clonal selection dominates medulloblastoma at recurrence. Nature. 2016;529:351-7 pubmed publisher
Aguilar A, Sun W, Liu L, Lu J, McEachern D, Bernard D, et al. Design of chemically stable, potent, and efficacious MDM2 inhibitors that exploit the retro-mannich ring-opening-cyclization reaction mechanism in spiro-oxindoles. J Med Chem. 2014;57:10486-98 pubmed publisher
Duarte L, Young A, Wang Z, Wu H, Panda T, Kou Y, et al. Histone H3.3 and its proteolytically processed form drive a cellular senescence programme. Nat Commun. 2014;5:5210 pubmed publisher
Krais A, Mühlbauer K, Kucab J, Chinbuah H, Cornelius M, Wei Q, et al. Comparison of the metabolic activation of environmental carcinogens in mouse embryonic stem cells and mouse embryonic fibroblasts. Toxicol In Vitro. 2015;29:34-43 pubmed publisher
Tran D, Bergholz J, Zhang H, He H, Wang Y, Zhang Y, et al. Insulin-like growth factor-1 regulates the SIRT1-p53 pathway in cellular senescence. Aging Cell. 2014;13:669-78 pubmed publisher
Wu J, Liang S, Bergholz J, He H, Walsh E, Zhang Y, et al. ?Np63? activates CD82 metastasis suppressor to inhibit cancer cell invasion. Cell Death Dis. 2014;5:e1280 pubmed publisher
Bohanon F, Wang X, Ding C, Ding Y, Radhakrishnan G, Rastellini C, et al. Oridonin inhibits hepatic stellate cell proliferation and fibrogenesis. J Surg Res. 2014;190:55-63 pubmed publisher
Cassol C, Winer D, Liu W, Guo M, Ezzat S, Asa S. Tyrosine kinase receptors as molecular targets in pheochromocytomas and paragangliomas. Mod Pathol. 2014;27:1050-62 pubmed publisher
Benson E, Mungamuri S, Attie O, Kracikova M, Sachidanandam R, Manfredi J, et al. p53-dependent gene repression through p21 is mediated by recruitment of E2F4 repression complexes. Oncogene. 2014;33:3959-69 pubmed publisher
Botchkina G, Zuniga E, Rowehl R, Park R, Bhalla R, Bialkowska A, et al. Prostate cancer stem cell-targeted efficacy of a new-generation taxoid, SBT-1214 and novel polyenolic zinc-binding curcuminoid, CMC2.24. PLoS ONE. 2013;8:e69884 pubmed publisher
El Karim E, Hagos E, Ghaleb A, Yu B, Yang V. Krüppel-like factor 4 regulates genetic stability in mouse embryonic fibroblasts. Mol Cancer. 2013;12:89 pubmed publisher
Hazawa M, Yasuda T, Noshiro K, Saotome Nakamura A, Fukuzaki T, Michikawa Y, et al. Vitronectin improves cell survival after radiation injury in human umbilical vein endothelial cells. FEBS Open Bio. 2012;2:334-8 pubmed publisher
Al Dhaheri Y, Eid A, Abuqamar S, Attoub S, Khasawneh M, Aiche G, et al. Mitotic arrest and apoptosis in breast cancer cells induced by Origanum majorana extract: upregulation of TNF-? and downregulation of survivin and mutant p53. PLoS ONE. 2013;8:e56649 pubmed publisher
Loponen H, Ylikoski J, Albrecht J, Pirvola U. Restrictions in cell cycle progression of adult vestibular supporting cells in response to ectopic cyclin D1 expression. PLoS ONE. 2011;6:e27360 pubmed publisher
Braun F, Bertin Ciftci J, Gallouet A, Millour J, Juin P. Serum-nutrient starvation induces cell death mediated by Bax and Puma that is counteracted by p21 and unmasked by Bcl-x(L) inhibition. PLoS ONE. 2011;6:e23577 pubmed publisher
Viarisio D, Mueller Decker K, Kloz U, Aengeneyndt B, Kopp Schneider A, Grone H, et al. E6 and E7 from beta HPV38 cooperate with ultraviolet light in the development of actinic keratosis-like lesions and squamous cell carcinoma in mice. PLoS Pathog. 2011;7:e1002125 pubmed publisher
Hamouchene H, Arlt V, Giddings I, Phillips D. Influence of cell cycle on responses of MCF-7 cells to benzo[a]pyrene. BMC Genomics. 2011;12:333 pubmed publisher
Kovacic J, Gupta R, Lee A, Ma M, Fang F, Tolbert C, et al. Stat3-dependent acute Rantes production in vascular smooth muscle cells modulates inflammation following arterial injury in mice. J Clin Invest. 2010;120:303-14 pubmed publisher
Bossuyt W, Kazanjian A, De Geest N, Van Kelst S, De Hertogh G, Geboes K, et al. Atonal homolog 1 is a tumor suppressor gene. PLoS Biol. 2009;7:e39 pubmed publisher
Rousseau E, Kojima R, Hoffner G, Djian P, Bertolotti A. Misfolding of proteins with a polyglutamine expansion is facilitated by proteasomal chaperones. J Biol Chem. 2009;284:1917-29 pubmed publisher
product information
Cat.# :
556431
Brand :
BD Pharmingen
Name :
p21
Format :
Purified
Clone :
SXM30
Isotype :
Ms IgG 1
React :
Hu , Ms , Rat
Conc :
0.5 mg/ml
Apps :
IF , IHC(F)* , IHC(Fr) , IP , WB
Size :
0.1 mg
Status :
RUO
more info or order :
company information