product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
CD63
catalog :
556019
quantity :
0.1 mg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
H5C6
reactivity :
African green monkey, human, mouse, brewer's yeast
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, other
more info or order :
citations: 38
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig 1a
Mathieu M, Nevo N, Jouve M, Valenzuela J, Maurin M, Verweij F, et al. Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9. Nat Commun. 2021;12:4389 pubmed publisher
  • western blot; human; 1:500; loading ...; fig s10d
Claude Taupin A, Jia J, Bhujabal Z, Garfa Traoré M, Kumar S, da Silva G, et al. ATG9A protects the plasma membrane from programmed and incidental permeabilization. Nat Cell Biol. 2021;23:846-858 pubmed publisher
  • immunohistochemistry - frozen section; human; fig 2d
Wu A, Sung Y, Chen Y, Chou S, Guo V, Chien J, et al. Multiresolution Imaging Using Bioluminescence Resonance Energy Transfer Identifies Distinct Biodistribution Profiles of Extracellular Vesicles and Exomeres with Redirected Tropism. Adv Sci (Weinh). 2020;7:2001467 pubmed publisher
  • flow cytometry; human; 1:20; loading ...; fig s4a, s4b
  • western blot; human; 1:1000; loading ...; fig 5f
Crescitelli R, Lässer C, Jang S, Cvjetkovic A, Malmhäll C, Karimi N, et al. Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation. J Extracell Vesicles. 2020;9:1722433 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 7a
  • western blot; human; loading ...; fig 1b, 3d, 3c 4h
Jeppesen D, Fenix A, Franklin J, Higginbotham J, Zhang Q, Zimmerman L, et al. Reassessment of Exosome Composition. Cell. 2019;177:428-445.e18 pubmed publisher
  • western blot; human; loading ...; fig 2f
Lee J, Dindorf J, Eberhardt M, Lai X, Ostalecki C, Koliha N, et al. Innate extracellular vesicles from melanoma patients suppress β-catenin in tumor cells by miRNA-34a. Life Sci Alliance. 2019;2: pubmed publisher
  • immunohistochemistry; human; loading ...; fig 3a
  • western blot; human; loading ...; fig 1c
Messenger S, Woo S, Sun Z, Martin T. A Ca2+-stimulated exosome release pathway in cancer cells is regulated by Munc13-4. J Cell Biol. 2018;217:2877-2890 pubmed publisher
  • western blot; human; loading ...; fig 6f
Verweij F, Bebelman M, Jimenez C, Garcia Vallejo J, Janssen H, Neefjes J, et al. Quantifying exosome secretion from single cells reveals a modulatory role for GPCR signaling. J Cell Biol. 2018;217:1129-1142 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1b
  • western blot; human; loading ...; fig 1a
Earnest J, Hantak M, Li K, McCray P, Perlman S, Gallagher T. The tetraspanin CD9 facilitates MERS-coronavirus entry by scaffolding host cell receptors and proteases. PLoS Pathog. 2017;13:e1006546 pubmed publisher
  • western blot; human; 1:500; fig 5c
Jong A, Wu C, Li J, Sun J, Fabbri M, Wayne A, et al. Large-scale isolation and cytotoxicity of extracellular vesicles derived from activated human natural killer cells. J Extracell Vesicles. 2017;6:1294368 pubmed publisher
  • immunocytochemistry; human; 1:2000; loading ...; fig 6a
Roy N, Pacini G, Berlioz Torrent C, Janvier K. Characterization of E3 ligases involved in lysosomal sorting of the HIV-1 restriction factor BST2. J Cell Sci. 2017;130:1596-1611 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 5a
Larson C, Heinzen R. High-Content Imaging Reveals Expansion of the Endosomal Compartment during Coxiella burnetii Parasitophorous Vacuole Maturation. Front Cell Infect Microbiol. 2017;7:48 pubmed publisher
  • immunocytochemistry; African green monkey; loading ...; fig s2
McLelland G, Lee S, McBride H, Fon E. Syntaxin-17 delivers PINK1/parkin-dependent mitochondrial vesicles to the endolysosomal system. J Cell Biol. 2016;214:275-91 pubmed publisher
  • immunocytochemistry; human; fig 1
Justis A, Hansen B, Beare P, King K, Heinzen R, Gilk S. Interactions between the Coxiella burnetii parasitophorous vacuole and the endoplasmic reticulum involve the host protein ORP1L. Cell Microbiol. 2017;19: pubmed publisher
  • western blot; brewer's yeast; 1:1000; fig s4
Andersson A, Andersson B, Lorell C, Raffetseder J, Larsson M, Blomgran R. Autophagy induction targeting mTORC1 enhances Mycobacterium tuberculosis replication in HIV co-infected human macrophages. Sci Rep. 2016;6:28171 pubmed publisher
  • western blot; human; 1:1000; fig 2
Wäster P, Eriksson I, Vainikka L, Rosdahl I, Ollinger K. Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation. Sci Rep. 2016;6:27890 pubmed publisher
  • immunocytochemistry; human; fig 4
Rapiteanu R, Davis L, Williamson J, Timms R, Paul Luzio J, Lehner P. A Genetic Screen Identifies a Critical Role for the WDR81-WDR91 Complex in the Trafficking and Degradation of Tetherin. Traffic. 2016;17:940-58 pubmed publisher
  • other; human; 500 ug/ml; fig 1
Belov L, Matic K, Hallal S, Best O, Mulligan S, Christopherson R. Extensive surface protein profiles of extracellular vesicles from cancer cells may provide diagnostic signatures from blood samples. J Extracell Vesicles. 2016;5:25355 pubmed publisher
  • flow cytometry; human; 10 ug/ml; fig 3
Khazen R, Müller S, Gaudenzio N, Espinosa E, Puissegur M, Valitutti S. Melanoma cell lysosome secretory burst neutralizes the CTL-mediated cytotoxicity at the lytic synapse. Nat Commun. 2016;7:10823 pubmed publisher
  • western blot; human; fig 2
Kowal J, Arras G, Colombo M, Jouve M, Morath J, Primdal Bengtson B, et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016;113:E968-77 pubmed publisher
  • immunocytochemistry; human; fig 1
Majumder P, Chakrabarti O. Mahogunin regulates fusion between amphisomes/MVBs and lysosomes via ubiquitination of TSG101. Cell Death Dis. 2015;6:e1970 pubmed publisher
  • flow cytometry; mouse; fig s1a
Phinney D, Di Giuseppe M, Njah J, Sala E, Shiva S, St Croix C, et al. Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle microRNAs. Nat Commun. 2015;6:8472 pubmed publisher
  • flow cytometry; human
Harshyne L, Nasca B, Kenyon L, Andrews D, Hooper D. Serum exosomes and cytokines promote a T-helper cell type 2 environment in the peripheral blood of glioblastoma patients. Neuro Oncol. 2016;18:206-15 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 6a
Hirst J, Edgar J, Esteves T, Darios F, Madeo M, Chang J, et al. Loss of AP-5 results in accumulation of aberrant endolysosomes: defining a new type of lysosomal storage disease. Hum Mol Genet. 2015;24:4984-96 pubmed publisher
  • immunoprecipitation; human; fig 2
Shao H, Chung J, Lee K, Balaj L, Min C, Carter B, et al. Chip-based analysis of exosomal mRNA mediating drug resistance in glioblastoma. Nat Commun. 2015;6:6999 pubmed publisher
  • flow cytometry; human; fig 2
Skogberg G, Lundberg V, Berglund M, Gudmundsdottir J, Telemo E, Lindgren S, et al. Human thymic epithelial primary cells produce exosomes carrying tissue-restricted antigens. Immunol Cell Biol. 2015;93:727-34 pubmed publisher
  • immunocytochemistry; human; 5 ug/ml
Vallabhaneni K, Penfornis P, Dhule S, Guillonneau F, Adams K, Mo Y, et al. Extracellular vesicles from bone marrow mesenchymal stem/stromal cells transport tumor regulatory microRNA, proteins, and metabolites. Oncotarget. 2015;6:4953-67 pubmed
  • immunocytochemistry; mouse
Chivet M, Javalet C, Laulagnier K, Blot B, Hemming F, Sadoul R. Exosomes secreted by cortical neurons upon glutamatergic synapse activation specifically interact with neurons. J Extracell Vesicles. 2014;3:24722 pubmed publisher
  • western blot; human; 1:1000
Lundholm M, Schröder M, Nagaeva O, Baranov V, Widmark A, Mincheva Nilsson L, et al. Prostate tumor-derived exosomes down-regulate NKG2D expression on natural killer cells and CD8+ T cells: mechanism of immune evasion. PLoS ONE. 2014;9:e108925 pubmed publisher
  • immunoprecipitation; human
  • immunoprecipitation; mouse
Shi M, Liu C, Cook T, Bullock K, Zhao Y, Ginghina C, et al. Plasma exosomal ?-synuclein is likely CNS-derived and increased in Parkinson's disease. Acta Neuropathol. 2014;128:639-650 pubmed publisher
  • flow cytometry; human
Lozano Fernández T, Ballester Antxordoki L, Pérez Temprano N, Rojas E, Sanz D, Iglesias Gaspar M, et al. Potential impact of metal oxide nanoparticles on the immune system: The role of integrins, L-selectin and the chemokine receptor CXCR4. Nanomedicine. 2014;10:1301-10 pubmed publisher
  • western blot; human; 1:1000
Armstrong A, Mattsson N, Appelqvist H, Janefjord C, Sandin L, Agholme L, et al. Lysosomal network proteins as potential novel CSF biomarkers for Alzheimer's disease. Neuromolecular Med. 2014;16:150-60 pubmed publisher
  • western blot; human; 1:150
Camacho L, Guerrero P, Marchetti D. MicroRNA and protein profiling of brain metastasis competent cell-derived exosomes. PLoS ONE. 2013;8:e73790 pubmed publisher
Zhong L, Song Y, Marziali F, Uzbekov R, Nguyen X, Journo C, et al. A novel domain within the CIL regulates egress of IFITM3 from the Golgi and reveals a regulatory role of IFITM3 on the secretory pathway. Life Sci Alliance. 2022;5: pubmed publisher
Kamerkar S, LeBleu V, Sugimoto H, Yang S, Ruivo C, Melo S, et al. Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer. Nature. 2017;546:498-503 pubmed publisher
Koliha N, Heider U, Ozimkowski T, Wiemann M, Bosio A, Wild S. Melanoma Affects the Composition of Blood Cell-Derived Extracellular Vesicles. Front Immunol. 2016;7:282 pubmed publisher
Zhu H, Guariglia S, Yu R, Li W, Brancho D, Peinado H, et al. Mutation of SIMPLE in Charcot-Marie-Tooth 1C alters production of exosomes. Mol Biol Cell. 2013;24:1619-37, S1-3 pubmed publisher
Kosaka N, Iguchi H, Hagiwara K, Yoshioka Y, Takeshita F, Ochiya T. Neutral sphingomyelinase 2 (nSMase2)-dependent exosomal transfer of angiogenic microRNAs regulate cancer cell metastasis. J Biol Chem. 2013;288:10849-59 pubmed publisher
product information
Cat.# :
556019
Brand :
BD Pharmingen
Name :
CD63
Format :
Purified
Clone :
H5C6
Isotype :
Ms IgG 1
React :
Hu
Conc :
0.5 mg/ml
Apps :
FCM , IHC(F)* , IHC(Fr
Size :
0.1 mg
Status :
RUO
more info or order :
company information