product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
CD95
catalog :
554254
quantity :
0.5 mg
clonality :
monoclonal
host :
hamsters
conjugate :
nonconjugated
clone name :
Jo2
reactivity :
mouse
application :
immunohistochemistry, flow cytometry, blocking or activating experiments
more info or order :
citations: 66
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 4b
Brown G, Ca xf1 ete P, Wang H, Medhavy A, Bones J, Roco J, et al. TLR7 gain-of-function genetic variation causes human lupus. Nature. 2022;605:349-356 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6e
Quách T, Huang W, Sahu R, Diadhiou C, Raparia C, Johnson R, et al. Context-dependent induction of autoimmunity by TNF signaling deficiency. JCI Insight. 2022;7: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Lin X, Twelkmeyer T, Zhu D, Zhang L, Zhao Y, Zhang C, et al. Homeostatic regulation of T follicular helper and antibody response to particle antigens by IL-1Ra of medullary sinus macrophage origin. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 2h, e2b
Aslam M, Alemdehy M, Kwesi Maliepaard E, Muhaimin F, Caganova M, Pardieck I, et al. Histone methyltransferase DOT1L controls state-specific identity during B cell differentiation. EMBO Rep. 2021;22:e51184 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Xu A, Barbosa R, Calado D. Genetic timestamping of plasma cells in vivo reveals tissue-specific homeostatic population turnover. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 4e
Cao W, Fang F, Gould T, Li X, Kim C, Gustafson C, et al. Ecto-NTPDase CD39 is a negative checkpoint that inhibits follicular helper cell generation. J Clin Invest. 2020;130:3422-3436 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7
Gryzik S, Hoang Y, Lischke T, Mohr E, Venzke M, Kadner I, et al. Identification of a super-functional Tfh-like subpopulation in murine lupus by pattern perception. elife. 2020;9: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 6d
Duplaquet L, Leroy C, Vinchent A, Paget S, Lefebvre J, Vanden Abeele F, et al. Control of cell death/survival balance by the MET dependence receptor. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6a
Bell O, Copland D, Ward A, Nicholson L, Lange C, Chu C, et al. Single Eye mRNA-Seq Reveals Normalisation of the Retinal Microglial Transcriptome Following Acute Inflammation. Front Immunol. 2019;10:3033 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6b
Marcandalli J, Fiala B, Ols S, Perotti M, De van der Schueren W, Snijder J, et al. Induction of Potent Neutralizing Antibody Responses by a Designed Protein Nanoparticle Vaccine for Respiratory Syncytial Virus. Cell. 2019;176:1420-1431.e17 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Faliti C, Gualtierotti R, Rottoli E, Gerosa M, Perruzza L, Romagnani A, et al. P2X7 receptor restrains pathogenic Tfh cell generation in systemic lupus erythematosus. J Exp Med. 2019;216:317-336 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Eldi P, Chaudhri G, Nutt S, Newsome T, Karupiah G. Viral Replicative Capacity, Antigen Availability via Hematogenous Spread, and High TFH:TFR Ratios Drive Induction of Potent Neutralizing Antibody Responses. J Virol. 2019;93: pubmed publisher
  • flow cytometry; mouse; loading ...; fig e1b
Karmaus P, Chen X, Lim S, Herrada A, Nguyen T, Xu B, et al. Metabolic heterogeneity underlies reciprocal fates of TH17 cell stemness and plasticity. Nature. 2019;565:101-105 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3d
Yao Y, Huang W, Li X, Li X, Qian J, Han H, et al. Tespa1 Deficiency Dampens Thymus-Dependent B-Cell Activation and Attenuates Collagen-Induced Arthritis in Mice. Front Immunol. 2018;9:965 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s2a
Yeh C, Nojima T, Kuraoka M, Kelsoe G. Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density. Nat Commun. 2018;9:928 pubmed publisher
  • flow cytometry; mouse; fig s1c
Mayer C, Gazumyan A, Kara E, Gitlin A, Golijanin J, Viant C, et al. The microanatomic segregation of selection by apoptosis in the germinal center. Science. 2017;358: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s2e, 7f
Yi W, Gupta S, Ricker E, Manni M, Jessberger R, Chinenov Y, et al. The mTORC1-4E-BP-eIF4E axis controls de novo Bcl6 protein synthesis in T cells and systemic autoimmunity. Nat Commun. 2017;8:254 pubmed publisher
  • flow cytometry; mouse; loading ...; tbl s1
Sinclair C, Bommakanti G, Gardinassi L, Loebbermann J, Johnson M, Hakimpour P, et al. mTOR regulates metabolic adaptation of APCs in the lung and controls the outcome of allergic inflammation. Science. 2017;357:1014-1021 pubmed publisher
  • flow cytometry; mouse; loading ...
Papa I, Saliba D, Ponzoni M, Bustamante S, Canete P, Gonzalez Figueroa P, et al. TFH-derived dopamine accelerates productive synapses in germinal centres. Nature. 2017;547:318-323 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4e
Lu P, Shih C, Qi H. Ephrin B1-mediated repulsion and signaling control germinal center T cell territoriality and function. Science. 2017;356: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 8b
Inoue T, Shinnakasu R, Ise W, Kawai C, Egawa T, Kurosaki T. The transcription factor Foxo1 controls germinal center B cell proliferation in response to T cell help. J Exp Med. 2017;214:1181-1198 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Gallagher S, Turman S, Lekstrom K, Wilson S, Herbst R, Wang Y. CD47 limits antibody dependent phagocytosis against non-malignant B cells. Mol Immunol. 2017;85:57-65 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s1c
Martínez Martín N, Maldonado P, Gasparrini F, Frederico B, Aggarwal S, Gaya M, et al. A switch from canonical to noncanonical autophagy shapes B cell responses. Science. 2017;355:641-647 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7
Chen S, Cai C, Li Z, Liu G, Wang Y, Blonska M, et al. Dissection of SAP-dependent and SAP-independent SLAM family signaling in NKT cell development and humoral immunity. J Exp Med. 2017;214:475-489 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2e
Morita K, Okamura T, Inoue M, Komai T, Teruya S, Iwasaki Y, et al. Egr2 and Egr3 in regulatory T cells cooperatively control systemic autoimmunity through Ltbp3-mediated TGF-β3 production. Proc Natl Acad Sci U S A. 2016;113:E8131-E8140 pubmed
  • flow cytometry; mouse; fig 5a
Milanovic M, Heise N, De Silva N, Anderson M, Silva K, Carette A, et al. Differential requirements for the canonical NF-?B transcription factors c-REL and RELA during the generation and activation of mature B cells. Immunol Cell Biol. 2017;95:261-271 pubmed publisher
  • blocking or activating experiments; mouse
  • flow cytometry; mouse; fig 2
Kong S, Yang Y, Xu Y, Wang Y, Zhang Y, Melo Cardenas J, et al. Endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 controls B-cell immunity through degradation of the death receptor CD95/Fas. Proc Natl Acad Sci U S A. 2016;113:10394-9 pubmed publisher
  • flow cytometry; mouse; fig s5
Wu J, Wu H, Tsai D, Chiang M, Chen Y, Gao S, et al. Temporal regulation of Lsp1 O-GlcNAcylation and phosphorylation during apoptosis of activated B cells. Nat Commun. 2016;7:12526 pubmed publisher
  • flow cytometry; mouse; fig s2
Wu S, Majeed S, Evans T, Camus M, Wong N, Schollmeier Y, et al. Clathrin light chains' role in selective endocytosis influences antibody isotype switching. Proc Natl Acad Sci U S A. 2016;113:9816-21 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
Liu W, Kang S, Huang Z, Wu C, Jin H, Maine C, et al. A miR-155-Peli1-c-Rel pathway controls the generation and function of T follicular helper cells. J Exp Med. 2016;213:1901-19 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3i
Jackson S, Jacobs H, Arkatkar T, Dam E, Scharping N, Kolhatkar N, et al. B cell IFN-γ receptor signaling promotes autoimmune germinal centers via cell-intrinsic induction of BCL-6. J Exp Med. 2016;213:733-50 pubmed publisher
  • flow cytometry; mouse; fig cd95
Leeth C, Racine J, Chapman H, Arpa B, Carrillo J, Carrascal J, et al. B-lymphocytes expressing an Ig specificity recognizing the pancreatic ß-cell autoantigen peripherin are potent contributors to type 1 diabetes development in NOD mice. Diabetes. 2016;65:1977-1987 pubmed publisher
  • flow cytometry; mouse; fig 3
Kabat A, Harrison O, Riffelmacher T, Moghaddam A, Pearson C, Laing A, et al. The autophagy gene Atg16l1 differentially regulates Treg and TH2 cells to control intestinal inflammation. elife. 2016;5:e12444 pubmed publisher
  • flow cytometry; mouse; 1:100; fig 2
Aloulou M, Carr E, Gador M, Bignon A, Liblau R, Fazilleau N, et al. Follicular regulatory T cells can be specific for the immunizing antigen and derive from naive T cells. Nat Commun. 2016;7:10579 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1D
Everts B, Tussiwand R, Dreesen L, Fairfax K, Huang S, Smith A, et al. Migratory CD103+ dendritic cells suppress helminth-driven type 2 immunity through constitutive expression of IL-12. J Exp Med. 2016;213:35-51 pubmed publisher
  • flow cytometry; mouse; fig 1
Fontinha D, Lopes F, Marques S, Alenquer M, Simas J. Murid Gammaherpesvirus Latency-Associated Protein M2 Promotes the Formation of Conjugates between Transformed B Lymphoma Cells and T Helper Cells. PLoS ONE. 2015;10:e0142540 pubmed publisher
  • flow cytometry; mouse; fig 6
Pei B, Zhao M, Miller B, Véla J, Bruinsma M, Virgin H, et al. Invariant NKT cells require autophagy to coordinate proliferation and survival signals during differentiation. J Immunol. 2015;194:5872-84 pubmed publisher
  • flow cytometry; mouse; 1:20
Lujan E, Zunder E, Ng Y, Goronzy I, Nolan G, Wernig M. Early reprogramming regulators identified by prospective isolation and mass cytometry. Nature. 2015;521:352-6 pubmed publisher
  • flow cytometry; mouse; 2:100; fig 1
Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, et al. TGF-β3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses. Nat Commun. 2015;6:6329 pubmed publisher
  • flow cytometry; mouse; fig 6
Cao A, Yao S, Gong B, Nurieva R, Elson C, Cong Y. Interleukin (IL)-21 promotes intestinal IgA response to microbiota. Mucosal Immunol. 2015;8:1072-82 pubmed publisher
  • flow cytometry; mouse
Dang V, Tanabe K, Tanaka Y, Tokumoto N, Misumi T, Saeki Y, et al. Fasting enhances TRAIL-mediated liver natural killer cell activity via HSP70 upregulation. PLoS ONE. 2014;9:e110748 pubmed publisher
  • flow cytometry; mouse
Chen M, Chen Y, Wu M, Yu G, Lin W, Tan T, et al. PP4 is essential for germinal center formation and class switch recombination in mice. PLoS ONE. 2014;9:e107505 pubmed publisher
  • flow cytometry; mouse; fig 2
Ishikura S, Ogawa M, Doi K, Matsuzaki H, Iwaihara Y, Tanaka Y, et al. Zfat-deficient CD4⁺ CD8⁺ double-positive thymocytes are susceptible to apoptosis with deregulated activation of p38 and JNK. J Cell Biochem. 2015;116:149-57 pubmed publisher
  • blocking or activating experiments; mouse; 10 ug/mouse
Strauss G, Westhoff M, Fischer Posovszky P, Fulda S, Schanbacher M, Eckhoff S, et al. 4-hydroperoxy-cyclophosphamide mediates caspase-independent T-cell apoptosis involving oxidative stress-induced nuclear relocation of mitochondrial apoptogenic factors AIF and EndoG. Cell Death Differ. 2008;15:332-43 pubmed
Weerasinghe S, Park M, Portney D, Omary M. Mouse genetic background contributes to hepatocyte susceptibility to Fas-mediated apoptosis. Mol Biol Cell. 2016;27:3005-3012 pubmed
Li Y, Takahashi Y, Fujii S, Zhou Y, Hong R, Suzuki A, et al. EAF2 mediates germinal centre B-cell apoptosis to suppress excessive immune responses and prevent autoimmunity. Nat Commun. 2016;7:10836 pubmed publisher
Kang Y, Bang B, Han K, Hong L, Shim E, Ma J, et al. Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling. Nat Commun. 2015;6:8371 pubmed publisher
Estornes Y, Aguileta M, Dubuisson C, De Keyser J, Goossens V, Kersse K, et al. RIPK1 promotes death receptor-independent caspase-8-mediated apoptosis under unresolved ER stress conditions. Cell Death Dis. 2014;5:e1555 pubmed publisher
Deegan S, Saveljeva S, Logue S, Pakos Zebrucka K, Gupta S, Vandenabeele P, et al. Deficiency in the mitochondrial apoptotic pathway reveals the toxic potential of autophagy under ER stress conditions. Autophagy. 2014;10:1921-36 pubmed publisher
Edgar C, Lindquist L, McKean D, Strasser A, Bram R. CAML regulates Bim-dependent thymocyte death. Cell Death Differ. 2010;17:1566-76 pubmed publisher
McKinstry K, Golech S, Lee W, Huston G, Weng N, Swain S. Rapid default transition of CD4 T cell effectors to functional memory cells. J Exp Med. 2007;204:2199-211 pubmed
Corcoran L, Hasbold J, Dietrich W, Hawkins E, Kallies A, Nutt S, et al. Differential requirement for OBF-1 during antibody-secreting cell differentiation. J Exp Med. 2005;201:1385-96 pubmed
Fox C, Hammerman P, Thompson C. The Pim kinases control rapamycin-resistant T cell survival and activation. J Exp Med. 2005;201:259-66 pubmed
Lohoff M, Mittrücker H, Brüstle A, Sommer F, Casper B, Huber M, et al. Enhanced TCR-induced apoptosis in interferon regulatory factor 4-deficient CD4(+) Th cells. J Exp Med. 2004;200:247-53 pubmed
Muriglan S, Ramirez Montagut T, Alpdogan O, Van Huystee T, Eng J, Hubbard V, et al. GITR activation induces an opposite effect on alloreactive CD4(+) and CD8(+) T cells in graft-versus-host disease. J Exp Med. 2004;200:149-57 pubmed
Pandiyan P, Gärtner D, Soezeri O, Radbruch A, Schulze Osthoff K, Brunner Weinzierl M. CD152 (CTLA-4) determines the unequal resistance of Th1 and Th2 cells against activation-induced cell death by a mechanism requiring PI3 kinase function. J Exp Med. 2004;199:831-42 pubmed
Seagal J, Edry E, Keren Z, Leider N, Benny O, Machluf M, et al. A fail-safe mechanism for negative selection of isotype-switched B cell precursors is regulated by the Fas/FasL pathway. J Exp Med. 2003;198:1609-19 pubmed
Goodyear C, Silverman G. Death by a B cell superantigen: In vivo VH-targeted apoptotic supraclonal B cell deletion by a Staphylococcal Toxin. J Exp Med. 2003;197:1125-39 pubmed
Refaeli Y, Van Parijs L, Alexander S, Abbas A. Interferon gamma is required for activation-induced death of T lymphocytes. J Exp Med. 2002;196:999-1005 pubmed
Jones R, Elford A, Parsons M, Wu L, Krawczyk C, Yeh W, et al. CD28-dependent activation of protein kinase B/Akt blocks Fas-mediated apoptosis by preventing death-inducing signaling complex assembly. J Exp Med. 2002;196:335-48 pubmed
Chen W, Jin W, Tian H, Sicurello P, Frank M, Orenstein J, et al. Requirement for transforming growth factor beta1 in controlling T cell apoptosis. J Exp Med. 2001;194:439-53 pubmed
Foghsgaard L, Wissing D, Mauch D, Lademann U, Bastholm L, Boes M, et al. Cathepsin B acts as a dominant execution protease in tumor cell apoptosis induced by tumor necrosis factor. J Cell Biol. 2001;153:999-1010 pubmed
Sabapathy K, Kallunki T, David J, Graef I, Karin M, Wagner E. c-Jun NH2-terminal kinase (JNK)1 and JNK2 have similar and stage-dependent roles in regulating T cell apoptosis and proliferation. J Exp Med. 2001;193:317-28 pubmed
Hisahara S, Yuan J, Momoi T, Okano H, Miura M. Caspase-11 mediates oligodendrocyte cell death and pathogenesis of autoimmune-mediated demyelination. J Exp Med. 2001;193:111-22 pubmed
Latta M, Künstle G, Leist M, Wendel A. Metabolic depletion of ATP by fructose inversely controls CD95- and tumor necrosis factor receptor 1-mediated hepatic apoptosis. J Exp Med. 2000;191:1975-85 pubmed
Komoriya A, Packard B, Brown M, Wu M, Henkart P. Assessment of caspase activities in intact apoptotic thymocytes using cell-permeable fluorogenic caspase substrates. J Exp Med. 2000;191:1819-28 pubmed
product information
Cat.# :
554254
Brand :
BD Pharmingen
Name :
CD95
Format :
NA/LE
Clone :
Jo2
Isotype :
Ar Ham IgG 2 , λ2
React :
Ms
Conc :
1.0 mg/ml
Apps :
FCM
Size :
0.5 mg
Status :
RUO
more info or order :
company information