product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
CD4
catalog :
553727
quantity :
0.5 mg
price :
225.00 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
GK1.5
reactivity :
human, mouse
application :
immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 52
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; loading ...; fig s2a, s2b
Aarts S, Seijkens T, Kusters P, Van Tiel C, Reiche M, den Toom M, et al. Macrophage CD40 signaling drives experimental autoimmune encephalomyelitis. J Pathol. 2018;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s18
Vendetti F, Karukonda P, Clump D, Teo T, Lalonde R, Nugent K, et al. ATR kinase inhibitor AZD6738 potentiates CD8+ T cell-dependent antitumor activity following radiation. J Clin Invest. 2018;128:3926-3940 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
  • immunohistochemistry; mouse; fig 5e
Stefani F, Eberstål S, Vergani S, Kristiansen T, Bengzon J. Low-dose irradiated mesenchymal stromal cells break tumor defensive properties in vivo. Int J Cancer. 2018;143:2200-2212 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 2a
Guimarães G, Gomes M, Campos P, Marinho F, de Assis N, Silveira T, et al. Immunoproteasome Subunits Are Required for CD8+ T Cell Function and Host Resistance to Brucella abortus Infection in Mice. Infect Immun. 2018;86: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Mailer R, Gisterå A, Polyzos K, Ketelhuth D, Hansson G. Hypercholesterolemia Enhances T Cell Receptor Signaling and Increases the Regulatory T Cell Population. Sci Rep. 2017;7:15655 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6c
Seifert H, Benedek G, Liang J, Nguyen H, Kent G, Vandenbark A, et al. Sex differences in regulatory cells in experimental stroke. Cell Immunol. 2017;318:49-54 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2
Torcellan T, Hampton H, Bailey J, Tomura M, Brink R, Chtanova T. In vivo photolabeling of tumor-infiltrating cells reveals highly regulated egress of T-cell subsets from tumors. Proc Natl Acad Sci U S A. 2017;114:5677-5682 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 4b
Hara T, Nakaoka H, Hayashi T, Mimura K, Hoshino D, Inoue M, et al. Control of metastatic niche formation by targeting APBA3/Mint3 in inflammatory monocytes. Proc Natl Acad Sci U S A. 2017;114:E4416-E4424 pubmed publisher
  • flow cytometry; mouse; fig 1d
Laffont S, Blanquart E, Savignac M, Cenac C, Laverny G, Metzger D, et al. Androgen signaling negatively controls group 2 innate lymphoid cells. J Exp Med. 2017;214:1581-1592 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4c
Chowdhary V, Krogman A, Tilahun A, Alexander M, David C, Rajagopalan G. Concomitant Disruption of CD4 and CD8 Genes Facilitates the Development of Double Negative ?? TCR+ Peripheral T Cells That Respond Robustly to Staphylococcal Superantigen. J Immunol. 2017;198:4413-4424 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1c
Berghoff S, Gerndt N, Winchenbach J, Stumpf S, Hosang L, Odoardi F, et al. Dietary cholesterol promotes repair of demyelinated lesions in the adult brain. Nat Commun. 2017;8:14241 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Wei Y, Lu C, Chen J, Cui G, Wang L, Yu T, et al. High salt diet stimulates gut Th17 response and exacerbates TNBS-induced colitis in mice. Oncotarget. 2017;8:70-82 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5c
Yanagisawa H, Hashimoto M, Minagawa S, Takasaka N, Ma R, Moermans C, et al. Role of IL-17A in murine models of COPD airway disease. Am J Physiol Lung Cell Mol Physiol. 2017;312:L122-L130 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1b
Coursey T, Bian F, Zaheer M, Pflugfelder S, Volpe E, de Paiva C. Age-related spontaneous lacrimal keratoconjunctivitis is accompanied by dysfunctional T regulatory cells. Mucosal Immunol. 2017;10:743-756 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1b
De Grove K, Provoost S, Hendriks R, McKenzie A, Seys L, Kumar S, et al. Dysregulation of type 2 innate lymphoid cells and TH2 cells impairs pollutant-induced allergic airway responses. J Allergy Clin Immunol. 2017;139:246-257.e4 pubmed publisher
  • flow cytometry; mouse; fig 6
Serr I, Fürst R, Ott V, Scherm M, Nikolaev A, Gökmen F, et al. miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity. Proc Natl Acad Sci U S A. 2016;113:E6659-E6668 pubmed
  • flow cytometry; mouse; loading ...; fig 4a
Chow K, Delconte R, Huntington N, Tarlinton D, Sutherland R, Zhan Y, et al. Innate Allorecognition Results in Rapid Accumulation of Monocyte-Derived Dendritic Cells. J Immunol. 2016;197:2000-8 pubmed publisher
  • flow cytometry; mouse; fig 5b
Keil M, Sonner J, Lanz T, Oezen I, Bunse T, Bittner S, et al. General control non-derepressible 2 (GCN2) in T cells controls disease progression of autoimmune neuroinflammation. J Neuroimmunol. 2016;297:117-26 pubmed publisher
  • flow cytometry; mouse
Kathania M, Khare P, Zeng M, Cantarel B, Zhang H, Ueno H, et al. Itch inhibits IL-17-mediated colon inflammation and tumorigenesis by ROR-?t ubiquitination. Nat Immunol. 2016;17:997-1004 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a
Sujino T, London M, Hoytema van Konijnenburg D, Rendon T, Buch T, Silva H, et al. Tissue adaptation of regulatory and intraepithelial CD4? T cells controls gut inflammation. Science. 2016;352:1581-6 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s8
Ueno M, Ueno Nakamura Y, Niehaus J, Popovich P, Yoshida Y. Silencing spinal interneurons inhibits immune suppressive autonomic reflexes caused by spinal cord injury. Nat Neurosci. 2016;19:784-7 pubmed publisher
  • flow cytometry; mouse
Maelfait J, Roose K, Vereecke L, Mc Guire C, Sze M, Schuijs M, et al. A20 Deficiency in Lung Epithelial Cells Protects against Influenza A Virus Infection. PLoS Pathog. 2016;12:e1005410 pubmed publisher
  • flow cytometry; mouse; fig 2b
Wei R, Hu Y, Dong F, Xu X, Hu A, Gao G. Hepatoma cell-derived leptin downregulates the immunosuppressive function of regulatory T-cells to enhance the anti-tumor activity of CD8+ T-cells. Immunol Cell Biol. 2016;94:388-99 pubmed publisher
  • flow cytometry; mouse; 1:200; fig 1
Francisconi C, Vieira A, Biguetti C, Glowacki A, Trombone A, Letra A, et al. Characterization of the Protective Role of Regulatory T Cells in Experimental Periapical Lesion Development and Their Chemoattraction Manipulation as a Therapeutic Tool. J Endod. 2016;42:120-6 pubmed publisher
  • flow cytometry; human; 1:200
Riesenberg S, Groetchen A, Siddaway R, Bald T, Reinhardt J, Smorra D, et al. MITF and c-Jun antagonism interconnects melanoma dedifferentiation with pro-inflammatory cytokine responsiveness and myeloid cell recruitment. Nat Commun. 2015;6:8755 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 7
Black L, Srivastava R, Schoeb T, Moore R, Barnes S, KABAROWSKI J. Cholesterol-Independent Suppression of Lymphocyte Activation, Autoimmunity, and Glomerulonephritis by Apolipoprotein A-I in Normocholesterolemic Lupus-Prone Mice. J Immunol. 2015;195:4685-98 pubmed publisher
  • flow cytometry; mouse; tbl s1
Kamachi F, Isshiki T, Harada N, Akiba H, Miyake S. ICOS promotes group 2 innate lymphoid cell activation in lungs. Biochem Biophys Res Commun. 2015;463:739-45 pubmed publisher
  • flow cytometry; mouse; fig 2
Yu J, Hoffman S, Beal A, Dykon A, Ringenberg M, Hughes A, et al. MALT1 Protease Activity Is Required for Innate and Adaptive Immune Responses. PLoS ONE. 2015;10:e0127083 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3A
Shao L, Lie A, Zhang Y, Wong C, Kwong Y. Aberrant germinal center formation, follicular T-helper cells, and germinal center B-cells were involved in chronic graft-versus-host disease. Ann Hematol. 2015;94:1493-504 pubmed publisher
  • flow cytometry; mouse; fig 2
  • immunocytochemistry; mouse; fig 4
Thiault N, Darrigues J, Adoue V, Gros M, Binet B, Pérals C, et al. Peripheral regulatory T lymphocytes recirculating to the thymus suppress the development of their precursors. Nat Immunol. 2015;16:628-34 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Becker P, Hervouet C, Mason G, KWON S, Klavinskis L. Skin vaccination with live virus vectored microneedle arrays induce long lived CD8(+) T cell memory. Vaccine. 2015;33:4691-8 pubmed publisher
  • flow cytometry; mouse
Sakala I, Chaudhri G, Eldi P, Buller R, Karupiah G. Deficiency in Th2 cytokine responses exacerbate orthopoxvirus infection. PLoS ONE. 2015;10:e0118685 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7a
Mang Y, Zhao Z, Zeng Z, Wu X, Li Z, Zhang L. Efficient elimination of CD103-expressing cells by anti-CD103 antibody drug conjugates in immunocompetent mice. Int Immunopharmacol. 2015;24:119-27 pubmed publisher
  • flow cytometry; mouse
Kim K, Skora A, Li Z, Liu Q, Tam A, Blosser R, et al. Eradication of metastatic mouse cancers resistant to immune checkpoint blockade by suppression of myeloid-derived cells. Proc Natl Acad Sci U S A. 2014;111:11774-9 pubmed publisher
  • flow cytometry; mouse
Vegran F, Berger H, Boidot R, Mignot G, Bruchard M, Dosset M, et al. The transcription factor IRF1 dictates the IL-21-dependent anticancer functions of TH9 cells. Nat Immunol. 2014;15:758-66 pubmed publisher
  • flow cytometry; mouse
Gaughan A, Wang J, Pelletier R, Nadasdy T, Brodsky S, Roy S, et al. Key role for CD4 T cells during mixed antibody-mediated rejection of renal allografts. Am J Transplant. 2014;14:284-94 pubmed publisher
  • flow cytometry; mouse
Hu Y, Xiao H, Shi T, Oppenheim J, Chen X. Progranulin promotes tumour necrosis factor-induced proliferation of suppressive mouse CD4? Foxp3? regulatory T cells. Immunology. 2014;142:193-201 pubmed publisher
  • flow cytometry; mouse
Qu S, Ou Yang H, He Y, Li Z, Shi J, Song L, et al. Der p2 recombinant bacille Calmette-Guerin priming of bone marrow-derived dendritic cells suppresses Der p2-induced T helper17 function in a mouse model of asthma. Respirology. 2014;19:122-31 pubmed publisher
  • immunohistochemistry; mouse
Weng T, Huang S, Yen M, Lin C, Chen Y, Lin C, et al. A novel cancer therapeutic using thrombospondin 1 in dendritic cells. Mol Ther. 2014;22:292-302 pubmed publisher
  • immunohistochemistry - frozen section; mouse
Coffey F, Manser T. Expression of cellular FLIP by B cells is required for their participation in an immune response. J Immunol. 2010;184:4871-9 pubmed publisher
  • flow cytometry; mouse; fig 1
Borsellino G, Kleinewietfeld M, Di Mitri D, Sternjak A, Diamantini A, Giometto R, et al. Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression. Blood. 2007;110:1225-32 pubmed
Wasiuk A, Testa J, Weidlick J, Sisson C, Vitale L, Widger J, et al. CD27-Mediated Regulatory T Cell Depletion and Effector T Cell Costimulation Both Contribute to Antitumor Efficacy. J Immunol. 2017;199:4110-4123 pubmed publisher
Seifert A, Zeng S, Zhang J, Kim T, Cohen N, Beckman M, et al. PD-1/PD-L1 Blockade Enhances T-cell Activity and Antitumor Efficacy of Imatinib in Gastrointestinal Stromal Tumors. Clin Cancer Res. 2017;23:454-465 pubmed publisher
Ko E, Lee Y, Kim K, Jung Y, Lee Y, Denning T, et al. Effects of MF59 Adjuvant on Induction of Isotype-Switched IgG Antibodies and Protection after Immunization with T-Dependent Influenza Virus Vaccine in the Absence of CD4+ T Cells. J Virol. 2016;90:6976-6988 pubmed publisher
Hermes G, Ajioka J, Kelly K, Mui E, Roberts F, Kasza K, et al. Neurological and behavioral abnormalities, ventricular dilatation, altered cellular functions, inflammation, and neuronal injury in brains of mice due to common, persistent, parasitic infection. J Neuroinflammation. 2008;5:48 pubmed publisher
Yu Q, Park J, Doan L, Erman B, Feigenbaum L, Singer A. Cytokine signal transduction is suppressed in preselection double-positive thymocytes and restored by positive selection. J Exp Med. 2006;203:165-75 pubmed
Cohen J, Trenado A, Vasey D, Klatzmann D, Salomon B. CD4(+)CD25(+) immunoregulatory T Cells: new therapeutics for graft-versus-host disease. J Exp Med. 2002;196:401-6 pubmed
Panigada M, Porcellini S, Barbier E, Hoeflinger S, Cazenave P, Gu H, et al. Constitutive endocytosis and degradation of the pre-T cell receptor. J Exp Med. 2002;195:1585-97 pubmed
Li Y, Wang M, Li H, King K, Bassi R, Sun H, et al. Active immunization against the vascular endothelial growth factor receptor flk1 inhibits tumor angiogenesis and metastasis. J Exp Med. 2002;195:1575-84 pubmed
Chung D, Chitnis T, Panzo R, Kasper D, Sayegh M, Tzianabos A. CD4+ T cells regulate surgical and postinfectious adhesion formation. J Exp Med. 2002;195:1471-8 pubmed
Yamazaki T, Takeda K, Gotoh K, Takeshima H, Akira S, Kurosaki T. Essential immunoregulatory role for BCAP in B cell development and function. J Exp Med. 2002;195:535-45 pubmed
Foucras G, Gapin L, Coureau C, Kanellopoulos J, Guery J. Interleukin 4-producing CD4 T cells arise from different precursors depending on the conditions of antigen exposure in vivo. J Exp Med. 2000;191:683-94 pubmed
product information
Cat.# :
553727
Brand :
BD Pharmingen
Name :
CD4
Format :
Purified
Clone :
GK1.5
Isotype :
Rat IgG 2b
React :
Ms
Conc :
0.5 mg/ml
Apps :
FCM
Size :
0.5 mg
Status :
RUO
List Price :
225.00 USD
more info or order :
company information