product summary
company name :
BD Biosciences
other brands :
Transduction Laboratories, PharMingen, Difco
product type :
antibody
product name :
Mac-3
catalog :
550292
quantity :
1 mL
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
M3/84
reactivity :
mouse
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 70
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 2d, 8a
Kettwig M, Ternka K, Wendland K, Krüger D, Zampar S, Schob C, et al. Interferon-driven brain phenotype in a mouse model of RNaseT2 deficient leukoencephalopathy. Nat Commun. 2021;12:6530 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 5j
Villanueva E, Tresse E, Liu Y, Duarte J, Jimenez Duran G, Ejlerskov P, et al. Neuronal TNFα, Not α-Syn, Underlies PDD-Like Disease Progression in IFNβ-KO Mice. Ann Neurol. 2021;90:789-807 pubmed publisher
  • immunohistochemistry; mouse; 2.5 ug/ml; loading ...; fig 2c
Datta M, Staszewski O. Hdac1 and Hdac2 are essential for physiological maturation of a Cx3cr1 expressing subset of T-lymphocytes. BMC Res Notes. 2021;14:135 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 4d
Wang X, Fu Y, Xie Z, Cao M, Qu W, Xi X, et al. Establishment of a Novel Mouse Model for Atherosclerotic Vulnerable Plaque. Front Cardiovasc Med. 2021;8:642751 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 3m
Jakubowska M, Pyka J, Michalczyk Wetula D, Baczynski K, Ciesla M, Susz A, et al. Electron paramagnetic resonance spectroscopy reveals alterations in the redox state of endogenous copper and iron complexes in photodynamic stress-induced ischemic mouse liver. Redox Biol. 2020;34:101566 pubmed publisher
  • immunohistochemistry; mouse; loading ...
Seitz C, Schroeder S, Knopf P, Krahl A, Hau J, Schleicher S, et al. GD2-targeted chimeric antigen receptor T cells prevent metastasis formation by elimination of breast cancer stem-like cells. Oncoimmunology. 2020;9:1683345 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig s10c
Schafflick D, Xu C, Hartlehnert M, Cole M, Schulte Mecklenbeck A, Lautwein T, et al. Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis. Nat Commun. 2020;11:247 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig 5a
Johnston J, Angyal A, Bauer R, Hamby S, Suvarna S, Baidžajevas K, et al. Myeloid Tribbles 1 induces early atherosclerosis via enhanced foam cell expansion. Sci Adv. 2019;5:eaax9183 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 8c
Mentrup T, Theodorou K, Cabrera Cabrera F, Helbig A, Happ K, Gijbels M, et al. Atherogenic LOX-1 signaling is controlled by SPPL2-mediated intramembrane proteolysis. J Exp Med. 2019;: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 8c
Jordão M, Sankowski R, Brendecke S, Sagar -, Locatelli G, Tai Y, et al. Single-cell profiling identifies myeloid cell subsets with distinct fates during neuroinflammation. Science. 2019;363: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:30; loading ...; fig 4e
Aarts S, Seijkens T, Kusters P, Van Tiel C, Reiche M, den Toom M, et al. Macrophage CD40 signaling drives experimental autoimmune encephalomyelitis. J Pathol. 2019;247:471-480 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; fig 2b
Bezhaeva T, De Vries M, Geelhoed W, van der Veer E, Versteeg S, van Alem C, et al. Relaxin receptor deficiency promotes vascular inflammation and impairs outward remodeling in arteriovenous fistulas. FASEB J. 2018;:fj201800437R pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:300; loading ...; fig 3g
Hoving L, De Vries M, de Jong R, Katiraei S, Pronk A, Quax P, et al. The Prebiotic Inulin Aggravates Accelerated Atherosclerosis in Hypercholesterolemic APOE*3-Leiden Mice. Nutrients. 2018;10: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 8c
Hulsmans M, Sager H, Roh J, Valero Muñoz M, Houstis N, Iwamoto Y, et al. Cardiac macrophages promote diastolic dysfunction. J Exp Med. 2018;215:423-440 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; fig s5a
Kojima Y, Volkmer J, McKenna K, Civelek M, Lusis A, Miller C, et al. CD47-blocking antibodies restore phagocytosis and prevent atherosclerosis. Nature. 2016;536:86-90 pubmed
  • western blot; mouse; fig 6
Inoue T, Ikeda M, Ide T, Fujino T, Matsuo Y, Arai S, et al. Twinkle overexpression prevents cardiac rupture after myocardial infarction by alleviating impaired mitochondrial biogenesis. Am J Physiol Heart Circ Physiol. 2016;311:H509-19 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig 6b
Al Sadoun H, Burgess M, Hentges K, Mace K. Enforced Expression of Hoxa3 Inhibits Classical and Promotes Alternative Activation of Macrophages In Vitro and In Vivo. J Immunol. 2016;197:872-84 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 3a
Kramann N, Menken L, Hayardeny L, Hanisch U, Bruck W. Laquinimod prevents cuprizone-induced demyelination independent of Toll-like receptor signaling. Neurol Neuroimmunol Neuroinflamm. 2016;3:e233 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig s2
Wezel A, De Vries M, Maassen J, Kip P, Peters E, Karper J, et al. Deficiency of the TLR4 analogue RP105 aggravates vein graft disease by inducing a pro-inflammatory response. Sci Rep. 2016;6:24248 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:300; fig 3
Zhang Z, Ren S, Tan Y, Li Z, Tang X, Wang T, et al. Epigenetic regulation of NKG2D ligands is involved in exacerbated atherosclerosis development in Sirt6 heterozygous mice. Sci Rep. 2016;6:23912 pubmed publisher
  • immunohistochemistry; mouse; 1:200; fig 5
Tjondrokoesoemo A, Schips T, Kanisicak O, Sargent M, Molkentin J. Genetic overexpression of Serpina3n attenuates muscular dystrophy in mice. Hum Mol Genet. 2016;25:1192-202 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500; fig 11
Nguyen K, McGilvray K, Puttlitz C, Mukhopadhyay S, Chabasse C, Sarkar R. Matrix Metalloproteinase 9 (MMP-9) Regulates Vein Wall Biomechanics in Murine Thrombus Resolution. PLoS ONE. 2015;10:e0139145 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:50; fig 1g
Yoshida Y, Shimizu I, Katsuumi G, Jiao S, Suda M, Hayashi Y, et al. p53-Induced inflammation exacerbates cardiac dysfunction during pressure overload. J Mol Cell Cardiol. 2015;85:183-98 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200
Wei K, Díaz Trelles R, Liu Q, Diez Cuñado M, Scimia M, Cai W, et al. Developmental origin of age-related coronary artery disease. Cardiovasc Res. 2015;107:287-94 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:50; fig 3
Khan I, Perrard X, Brunner G, Lui H, Sparks L, Smith S, et al. Intermuscular and perimuscular fat expansion in obesity correlates with skeletal muscle T cell and macrophage infiltration and insulin resistance. Int J Obes (Lond). 2015;39:1607-18 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 2
Sendler M, Beyer G, Mahajan U, Kauschke V, Maertin S, Schurmann C, et al. Complement Component 5 Mediates Development of Fibrosis, via Activation of Stellate Cells, in 2 Mouse Models of Chronic Pancreatitis. Gastroenterology. 2015;149:765-76.e10 pubmed publisher
  • immunocytochemistry; mouse; 1:10; fig 2
Tsuneki M, Hardee S, Michaud M, Morotti R, Lavik E, Madri J. A hydrogel-endothelial cell implant mimics infantile hemangioma: modulation by survivin and the Hippo pathway. Lab Invest. 2015;95:765-80 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 1
Heinzmann D, Bangert A, Müller A, von Ungern Sternberg S, Emschermann F, Schönberger T, et al. The Novel Extracellular Cyclophilin A (CyPA) - Inhibitor MM284 Reduces Myocardial Inflammation and Remodeling in a Mouse Model of Troponin I -Induced Myocarditis. PLoS ONE. 2015;10:e0124606 pubmed publisher
  • immunocytochemistry; mouse; 1:50
Huang L, Zhang S, Zhang P, Zhang X, Zhu L, Chen K, et al. Interferon regulatory factor 7 protects against vascular smooth muscle cell proliferation and neointima formation. J Am Heart Assoc. 2014;3:e001309 pubmed publisher
  • flow cytometry; mouse
Bernasconi E, D Angelo F, Michetti P, Velin D. Critical role of the GM-CSF signaling pathway in macrophage pro-repair activities. Pathobiology. 2014;81:183-9 pubmed publisher
  • immunohistochemistry; mouse
Vogelgesang A, Scapin C, Barone C, Tam E, Blumental Perry A, Dammann C. Cigarette smoke exposure during pregnancy alters fetomaternal cell trafficking leading to retention of microchimeric cells in the maternal lung. PLoS ONE. 2014;9:e88285 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
Aoshiba K, Tsuji T, Itoh M, Semba S, Yamaguchi K, Nakamura H, et al. A murine model of airway fibrosis induced by repeated naphthalene exposure. Exp Toxicol Pathol. 2014;66:169-77 pubmed publisher
  • immunohistochemistry; mouse
Sastre C, Fernández Laso V, Madrigal Matute J, Munoz Garcia B, Moreno J, Pastor Vargas C, et al. Genetic deletion or TWEAK blocking antibody administration reduce atherosclerosis and enhance plaque stability in mice. J Cell Mol Med. 2014;18:721-34 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100
Yanez C, Morales A, Yue X, Urakami T, Komatsu M, Järvinen T, et al. Deep vascular imaging in wounds by two-photon fluorescence microscopy. PLoS ONE. 2013;8:e67559 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
Aoshiba K, Tsuji T, Kameyama S, Itoh M, Semba S, Yamaguchi K, et al. Senescence-associated secretory phenotype in a mouse model of bleomycin-induced lung injury. Exp Toxicol Pathol. 2013;65:1053-62 pubmed publisher
Seifert R, Kuhlmann M, Eligehausen S, Kiefer F, Hermann S, Schäfers M. Molecular imaging of MMP activity discriminates unstable from stable plaque phenotypes in shear-stress induced murine atherosclerosis. PLoS ONE. 2018;13:e0204305 pubmed publisher
Gran S, Honold L, Fehler O, Zenker S, Eligehausen S, Kuhlmann M, et al. Imaging, myeloid precursor immortalization, and genome editing for defining mechanisms of leukocyte recruitment in vivo. Theranostics. 2018;8:2407-2423 pubmed publisher
Draijer C, Robbe P, Boorsma C, Hylkema M, Melgert B. Dual role of YM1+ M2 macrophages in allergic lung inflammation. Sci Rep. 2018;8:5105 pubmed publisher
Wang Q, He Z, Huang M, Liu T, Wang Y, Xu H, et al. Vascular niche IL-6 induces alternative macrophage activation in glioblastoma through HIF-2α. Nat Commun. 2018;9:559 pubmed publisher
Ullah K, Rosendahl A, Izzi V, Bergmann U, Pihlajaniemi T, Maki J, et al. Hypoxia-inducible factor prolyl-4-hydroxylase-1 is a convergent point in the reciprocal negative regulation of NF-κB and p53 signaling pathways. Sci Rep. 2017;7:17220 pubmed publisher
Xian X, Ding Y, Dieckmann M, Zhou L, Plattner F, Liu M, et al. LRP1 integrates murine macrophage cholesterol homeostasis and inflammatory responses in atherosclerosis. elife. 2017;6: pubmed publisher
Doran A, Ozcan L, Cai B, Zheng Z, Fredman G, Rymond C, et al. CAMKIIγ suppresses an efferocytosis pathway in macrophages and promotes atherosclerotic plaque necrosis. J Clin Invest. 2017;127:4075-4089 pubmed publisher
Wang Y, Subramanian M, Yurdagul A, Barbosa Lorenzi V, Cai B, de Juan Sanz J, et al. Mitochondrial Fission Promotes the Continued Clearance of Apoptotic Cells by Macrophages. Cell. 2017;171:331-345.e22 pubmed publisher
Ravindran D, Ridiandries A, Vanags L, Henriquez R, Cartland S, Tan J, et al. Chemokine binding protein 'M3' limits atherosclerosis in apolipoprotein E-/- mice. PLoS ONE. 2017;12:e0173224 pubmed publisher
Espitia Pinzon N, Sanz Morello B, Brevé J, Bol J, Drukarch B, Bauer J, et al. Astrocyte-derived tissue Transglutaminase affects fibronectin deposition, but not aggregation, during cuprizone-induced demyelination. Sci Rep. 2017;7:40995 pubmed publisher
Vogel A, Knier B, Lammens K, Kalluri S, Kuhlmann T, Bennett J, et al. Deletional tolerance prevents AQP4-directed autoimmunity in mice. Eur J Immunol. 2017;47:458-469 pubmed publisher
Zhao D, Tong L, Zhang L, Li H, Wan Y, Zhang T. Tanshinone II A stabilizes vulnerable plaques by suppressing RAGE signaling and NF-?B activation in apolipoprotein-E-deficient mice. Mol Med Rep. 2016;14:4983-4990 pubmed publisher
Iwata H, Goettsch C, Sharma A, Ricchiuto P, Goh W, Halu A, et al. PARP9 and PARP14 cross-regulate macrophage activation via STAT1 ADP-ribosylation. Nat Commun. 2016;7:12849 pubmed publisher
Klotz L, Kuzmanov I, Hucke S, Gross C, Posevitz V, Dreykluft A, et al. B7-H1 shapes T-cell-mediated brain endothelial cell dysfunction and regional encephalitogenicity in spontaneous CNS autoimmunity. Proc Natl Acad Sci U S A. 2016;113:E6182-E6191 pubmed
Tanaka S, Matsumoto T, Matsubara Y, Harada Y, Kyuragi R, Koga J, et al. BubR1 Insufficiency Results in Decreased Macrophage Proliferation and Attenuated Atherogenesis in Apolipoprotein E-Deficient Mice. J Am Heart Assoc. 2016;5: pubmed
Mukai K, Urai T, Asano K, Nakajima Y, Nakatani T. Evaluation of Effects of Topical Estradiol Benzoate Application on Cutaneous Wound Healing in Ovariectomized Female Mice. PLoS ONE. 2016;11:e0163560 pubmed publisher
Yang C, Tian A, Li Z. Reversible cardiac hypertrophy induced by PEG-coated gold nanoparticles in mice. Sci Rep. 2016;6:20203 pubmed publisher
Kusters D, Chatrou M, Willems B, de Saint Hubert M, Bauwens M, van der Vorst E, et al. Pharmacological Treatment with Annexin A1 Reduces Atherosclerotic Plaque Burden in LDLR-/- Mice on Western Type Diet. PLoS ONE. 2015;10:e0130484 pubmed publisher
Ueda K, Yoshimura K, Yamashita O, Harada T, Morikage N, Hamano K. Possible dual role of decorin in abdominal aortic aneurysm. PLoS ONE. 2015;10:e0120689 pubmed publisher
Yan H, Zhao L, Wu X, Liu H, Wu C, Li Y, et al. Inflammation and pathological damage to the lungs of mice are only partially reversed following smoking cessation on subacute exposure to cigarette smoke. Mol Med Rep. 2015;11:4246-54 pubmed publisher
Mukai K, Komatsu E, Nakajima Y, Urai T, Nasruddin -, Sugama J, et al. The effect of 17β-estradiol on cutaneous wound healing in protein-malnourished ovariectomized female mouse model. PLoS ONE. 2014;9:e115564 pubmed publisher
Chueh F, Lin J, Lin J, Weng S, Huang Y, Chung J. Crude extract of Polygonum cuspidatum stimulates immune responses in normal mice by increasing the percentage of Mac-3-positive cells and enhancing macrophage phagocytic activity and natural killer cell cytotoxicity. Mol Med Rep. 2015;11:127-32 pubmed publisher
van den Borne P, Rygiel T, Hoogendoorn A, Westerlaken G, Boon L, Quax P, et al. The CD200-CD200 receptor inhibitory axis controls arteriogenesis and local T lymphocyte influx. PLoS ONE. 2014;9:e98820 pubmed publisher
Huang M, Gong Y, Grondolsky J, Hoover Plow J. Lp(a)/apo(a) modulate MMP-9 activation and neutrophil cytokines in vivo in inflammation to regulate leukocyte recruitment. Am J Pathol. 2014;184:1503-17 pubmed publisher
Siu K, Miao X, Cai H. Recoupling of eNOS with folic acid prevents abdominal aortic aneurysm formation in angiotensin II-infused apolipoprotein E null mice. PLoS ONE. 2014;9:e88899 pubmed publisher
Raffay T, Locy M, Hill C, Jindal N, Rogers L, Welty S, et al. Neonatal hyperoxic exposure persistently alters lung secretoglobins and annexin A1. Biomed Res Int. 2013;2013:408485 pubmed publisher
Guo W, Wong S, Bhasin S. AAV-mediated administration of myostatin pro-peptide mutant in adult Ldlr null mice reduces diet-induced hepatosteatosis and arteriosclerosis. PLoS ONE. 2013;8:e71017 pubmed publisher
Govender P, Romero F, Shah D, Paez J, Ding S, Liu L, et al. Cavin1; a regulator of lung function and macrophage phenotype. PLoS ONE. 2013;8:e62045 pubmed publisher
Meisner J, Song J, Price R. Arteriolar and venular remodeling are differentially regulated by bone marrow-derived cell-specific CX3CR1 and CCR2 expression. PLoS ONE. 2012;7:e46312 pubmed publisher
Wielinga P, Yakala G, Heeringa P, Kleemann R, Kooistra T. Beneficial effects of alternate dietary regimen on liver inflammation, atherosclerosis and renal activation. PLoS ONE. 2011;6:e18432 pubmed publisher
Watt V, Chamberlain J, Steiner T, Francis S, Crossman D. TRAIL attenuates the development of atherosclerosis in apolipoprotein E deficient mice. Atherosclerosis. 2011;215:348-54 pubmed publisher
Aharonowiz M, Einstein O, Fainstein N, Lassmann H, Reubinoff B, Ben Hur T. Neuroprotective effect of transplanted human embryonic stem cell-derived neural precursors in an animal model of multiple sclerosis. PLoS ONE. 2008;3:e3145 pubmed publisher
Nahrendorf M, Swirski F, Aikawa E, Stangenberg L, Wurdinger T, Figueiredo J, et al. The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions. J Exp Med. 2007;204:3037-47 pubmed
Agrawal S, Anderson P, Durbeej M, Van Rooijen N, Ivars F, Opdenakker G, et al. Dystroglycan is selectively cleaved at the parenchymal basement membrane at sites of leukocyte extravasation in experimental autoimmune encephalomyelitis. J Exp Med. 2006;203:1007-19 pubmed
Walter M, Kajiwara N, Karanja P, Castro M, Holtzman M. Interleukin 12 p40 production by barrier epithelial cells during airway inflammation. J Exp Med. 2001;193:339-51 pubmed
product information
Cat.# :
550292
Brand :
BD Pharmingen
Name :
Mac-3
Format :
Purified
Clone :
M3/84
Isotype :
Rat IgG 1
React :
Ms
Conc :
31.25 µg/ml
Apps :
FCM , IHC(P) , IHC(Zn
Size :
1 mL
Status :
RUO
more info or order :
company information