protein

Recombinant Human Factor VIII Protein (OPPA01234)

OPPA01234

250 IU

Protein

Protein

Recombinant Human Factor VIII Protein (OPPA01234)

250 IU

FACTOR-VIII

Coagulation factor VIII, Procoagulant component, Antihemophilic factor, AHF, F8, F8C, F8B, HEMA, FVIII, DXS1253E, F8 protein, Factor-VIII Human Recombinant, Factor-VIII Human Recombinant

NP_000123.1

P00451

Lyophilized. Each IU was lyophilized from a solution containing 30ug PEG, 1.6ng VWF, 4ug calcium, and 0.25mg HSA.Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

It is recommended to reconstitute lyophilized Factor-VIII in 5ml sterile 18M-cm H2O, which can then be further diluted to other aqueous solutions. Lyophilized Factor-VIII although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Factor-VIII should be stored at 4C between 2-7 days and for future use below -18C. Please prevent freeze-thaw cycles.

Coagulation factor VIII participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.

Chinese Hamster Ovarian Cells (CHO)

The specific activity was found to be 7,058 IU/mg.

Greater than 97.0% as determined by SDS-PAGE.

Room Temperature

-18C