ELISA/assay

LPCAT1 ELISA Kit (Human) (OKCD01120)

OKCD01120

96 Wells

Application Kit

ELISA Kits

LPCAT1 ELISA Kit (Human) (OKCD01120)

96 Wells

LPCAT1

1-acylglycerophosphocholine O-acyltransferase, 1-alkylglycerophosphocholine O-acetyltransferase, Acetyl-CoA:lyso-PAF acetyltransferase, Acetyl-CoA:lyso-platelet-activating factor acetyltransferase, Acyltransferase-like 2, AYTL2, FLJ12443, FLJ41609, LPC ac

NM_024830.3

NP_079106

Q8NF37

Human

Store as indicated in product manual.

Possesses both acyltransferase and acetyltransferase activities (PubMed:16864775, PubMed:2149855). Activity is calcium-independent (By similarity). Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC) (PubMed:2149855). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively (PubMed:1674971). May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology (PubMed:16864775). Involved in the regulation of lipid droplet number and size (PubMed:25491198).By similarity p>Manually curated information which has been propagated from a related experimentally characterized protein. /p> a href="/manual/evidences#ECO:25">More… /a> /p> Manual assertion inferred from sequence similarity toiUniProtKB:Q3TFD2 (PCAT1_MOUSE)3 Publications p>Manually curated information for which there is published experimental evidence. /p> a href="/manual/evidences#ECO:269">More… /a> /p> Manual assertion based on experiment iniRef.7"Identification and characterization of a lysophosphatidylcholine acyltransferase in alveolar type II cells." Chen X., Hyatt B.A., Mucenski M.L., Mason R.J., Shannon J.M. Proc. Natl. Acad. Sci. U.S.A. 13:11724-11729(26) [PubMed] [Europe PMC] [Abstract]Cited for: IDENTIFICATION, FUNCTION.Ref.9"Human lysophosphatidylcholine acyltransferases 1 and 2 are located in lipid droplets where they catalyze the formation of phosphatidylcholine." Moessinger C., Kuerschner L., Spandl J., Shevchenko A., Thiele C. J. Biol. Chem. 286:2133-21339(211) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY, SUBCELLULAR LOCATION, TOPOLOGY.Ref.1"Two different pathways of phosphatidylcholine synthesis, the Kennedy Pathway and the Lands Cycle, differentially regulate cellular triacylglycerol storage." Moessinger C., Klizaite K., Steinhagen A., Philippou-Massier J., Shevchenko A., Hoch M., Ejsing C.S., Thiele C. BMC Cell Biol. 15:43-43(214) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.

ELISA

Assay Methodology: Quantitative Sandwich Immunoassay

< 1.15 ng/mL

This assay has high sensitivity and excellent specificity for detection of Lysophosphatidylcholine Acyltransferase 1 (LPCAT1). No significant cross-reactivity or interference between Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) and analogues was observed.

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