ELISA/assay

EZH2 ELISA Kit (Human) (OKCD00588)

OKCD00588

96 Wells

Application Kit

ELISA Kits

EZH2 ELISA Kit (Human) (OKCD00588)

96 Wells

EZH2

Enhancer of zeste homolog 2, ENX1, ENX-1, EZH1, Histone-lysine N-methyltransferase EZH2, KMT6, KMT6A, Lysine N-methyltransferase 6, MGC9169

NM_001203247.1

NP_001190176

Q15910

Human

Store as indicated in product manual.

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.16 Publications p>Manually curated information for which there is published experimental evidence. /p> a href="/manual/evidences#ECO:269">More… /a> /p> Manual assertion based on experiment iniRef.15"EZH2 is downstream of the pRB-E2F pathway, essential for proliferation and amplified in cancer." Bracken A.P., Pasini D., Capra M., Prosperini E., Colli E., Helin K. EMBO J. 22:5323-5335(23) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION, TISSUE SPECIFICITY.Ref.16"Suz12 is essential for mouse development and for EZH2 histone methyltransferase activity." Pasini D., Bracken A.P., Jensen M.R., Lazzerini Denchi E., Helin K. EMBO J. 23:461-471(24) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH EED AND SUZ12, METHYLTRANSFERASE ACTIVITY OF THE PRC2 COMPLEX.Ref.17"Silencing of human polycomb target genes is associated with methylation of histone H3 Lys 27." Kirmizis A., Bartley S.M., Kuzmichev A., Margueron R., Reinberg D., Green R., Farnham P.J. Genes Dev. 18:1592-165(24) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBCELLULAR LOCATION.Ref.19"SUZ12 is required for both the histone methyltransferase activity and the silencing function of the EED-EZH2 complex." Cao R., Zhang Y. Mol. Cell 15:57-67(24) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, CHARACTERIZATION OF THE PRC2 COMPLEX INCLUDING AEBP2; EED; EZH2; RBBP4 AND SUZ12, METHYLTRANSFERASE ACTIVITY OF THE PRC2 COMPLEX.Ref.21"The human tumor antigen PRAME is a dominant repressor of retinoic acid receptor signaling." Epping M.T., Wang L., Edel M.J., Carlee L., Hernandez M., Bernards R. Cell 122:835-847(25) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN RETINOIC ACID SIGNALING, INTERACTION WITH PRAME.Ref.25"Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions." Bracken A.P., Dietrich N., Pasini D., Hansen K.H., Helin K. Genes Dev. 2:1123-1136(26) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.27"The polycomb group protein EZH2 is required for mammalian circadian clock function." Etchegaray J.P., Yang X., DeBruyne J.P., Peters A.H., Weaver D.R., Jenuwein T., Reppert S.M. J. Biol. Chem. 281:2129-21215(26) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.29"The Polycomb group protein EZH2 directly controls DNA methylation." Vire E., Brenner C., Deplus R., Blanchon L., Fraga M., Didelot C., Morey L., Van Eynde A., Bernard D., Vanderwinden J.-M., Bollen M., Esteller M., Di Croce L., de Launoit Y., Fuks F. Nature 439:871-874(26) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION OF THE PRC2 COMPLEX WITH DNMT1; DNMT3A AND DNMT3B.Ref.3"Argonaute-1 directs siRNA-mediated transcriptional gene silencing in human cells." Kim D.H., Villeneuve L.M., Morris K.V., Rossi J.J. Nat. Struct. Mol. Biol. 13:793-797(26) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.31"pRB family proteins are required for H3K27 trimethylation and Polycomb repression complexes binding to and silencing p16INK4alpha tumor suppressor gene." Kotake Y., Cao R., Viatour P., Sage J., Zhang Y., Xiong Y. Genes Dev. 21:49-54(27) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.32"The Polycomb group proteins bind throughout the INK4A-ARF locus and are disassociated in senescent cells." Bracken A.P., Kleine-Kohlbrecher D., Dietrich N., Pasini D., Gargiulo G., Beekman C., Theilgaard-Moench K., Minucci S., Porse B.T., Marine J.-C., Hansen K.H., Helin K. Genes Dev. 21:525-53(27) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, DEVELOPMENTAL STAGE, INDUCTION.Ref.36"Ezh1 and Ezh2 maintain repressive chromatin through different mechanisms." Margueron R., Li G., Sarma K., Blais A., Zavadil J., Woodcock C.L., Dynlacht B.D., Reinberg D. Mol. Cell 32:53-518(28) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, IDENTIFICATION IN THE PRC2/EED-EZH1 COMPLEX.Ref.38"Ezh2 requires PHF1 to efficiently catalyze H3 lysine 27 trimethylation in vivo." Sarma K., Margueron R., Ivanov A., Pirrotta V., Reinberg D. Mol. Cell. Biol. 28:2718-2731(28) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH EED; SUZ12 AND PHF1, METHYLTRANSFERASE ACTIVITY OF THE PRC2 COMPLEX, MUTAGENESIS OF HIS-689.Ref.43"Cyclin-dependent kinases regulate epigenetic gene silencing through phosphorylation of EZH2." Chen S., Bohrer L.R., Rai A.N., Pan Y., Gan L., Zhou X., Bagchi A., Simon J.A., Huang H. Nat. Cell Biol. 12:118-1114(21) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, PHOSPHORYLATION AT THR-345 BY CDK1 AND CDK2, MUTAGENESIS OF THR-345.Ref.47"EZH2 generates a methyl degron that is recognized by the DCAF1/DDB1/CUL4 E3 ubiquitin ligase complex." Lee J.M., Lee J.S., Kim H., Kim K., Park H., Kim J.Y., Lee S.H., Kim I.S., Kim J., Lee M., Chung C.H., Seo S.B., Yoon J.B., Ko E., Noh D.Y., Kim K.I., Kim K.K., Baek S.H. Mol. Cell 48:572-586(212) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.49"O-GlcNAcylation regulates EZH2 protein stability and function." Chu C.S., Lo P.W., Yeh Y.H., Hsu P.H., Peng S.H., Teng Y.C., Kang M.L., Wong C.H., Juan L.J. Proc. Natl. Acad. Sci. U.S.A. 111:1355-136(214) [PubMed] [Europe PMC] [Abstract]Cited for: GLYCOSYLATION AT SER-75, MUTAGENESIS OF SER-75, FUNCTION.

ELISA

Assay Methodology: Quantitative Sandwich Immunoassay

< 0.063 ng/mL

This assay has high sensitivity and excellent specificity for detection of Enhancer Of Zeste Homolog 2 (EZH2). No significant cross-reactivity or interference between Enhancer Of Zeste Homolog 2 (EZH2) and analogues was observed.

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4C