ELISA/assay

IRF3 ELISA Kit (Mouse) (OKCD00283)

OKCD00283

96 Wells

Application Kit

ELISA Kits

IRF3 ELISA Kit (Mouse) (OKCD00283)

96 Wells

IRF3

C920001K05Rik, Interferon regulatory factor 3, IRF-3, MGC91046

NM_016849.4

NP_058545

P70671

Mouse

Store as indicated in product manual.

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.1 Publication p>Manually curated information for which there is published experimental evidence. /p> a href="/manual/evidences#ECO:269">More… /a> /p> Manual assertion based on experiment iniRef.3"IRF-7 is the master regulator of type-I interferon-dependent immune responses." Honda K., Yanai H., Negishi H., Asagiri M., Sato M., Mizutani T., Shimada N., Ohba Y., Takaoka A., Yoshida N., Taniguchi T. Nature 434:772-777(25) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.

ELISA

Assay Methodology: Quantitative Sandwich Immunoassay

< 0.055 ng/mL

This assay has high sensitivity and excellent specificity for detection of Interferon Regulatory Factor 3 (IRF3). No significant cross-reactivity or interference between Interferon Regulatory Factor 3 (IRF3) and analogues was observed.

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