ELISA/assay

RNASEL ELISA Kit (Human) (OKCD00189)

OKCD00189

96 Wells

Application Kit

ELISA Kits

RNASEL ELISA Kit (Human) (OKCD00189)

96 Wells

RNASEL

2-5A-dependent ribonuclease, 2-5A-dependent RNase, DKFZp781D08126, hereditary prostate cancer 1, HPC1, MGC104972, MGC133329, PRCA1, Ribonuclease 4, Ribonuclease L, RNase L, RNS4

NM_021133.3

NP_066956

Q05823

Human

Store as indicated in product manual.

Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis (PubMed:26263979). Might play a central role in the regulation of mRNA turnover (PubMed:11585831).2 Publications p>Manually curated information for which there is published experimental evidence. /p> a href="/manual/evidences#ECO:269">More… /a> /p> Manual assertion based on experiment iniRef.9"The 2-5A/RNase L/RNase L inhibitor (RNI) pathway regulates mitochondrial mRNAs stability in interferon alpha-treated H9 cells." Le Roy F., Bisbal C., Silhol M., Martinand C., Lebleu B., Salehzada T. J. Biol. Chem. 276:48473-48482(21) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBCELLULAR LOCATION.Ref.14"RNase L cleavage products promote switch from autophagy to apoptosis by caspase-mediated cleavage of beclin-1." Siddiqui M.A., Mukherjee S., Manivannan P., Malathi K. Int. J. Mol. Sci. 16:17611-17636(215) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.

ELISA

Assay Methodology: Quantitative Sandwich Immunoassay

< 6.5 pg/mL

This assay has high sensitivity and excellent specificity for detection of Ribonuclease L (RNASEL). No significant cross-reactivity or interference between Ribonuclease L (RNASEL) and analogues was observed.

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