GABRA1 Antibody (OAPC00118) | Aviva Systems Biology OAPC00118 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Aviva Systems Biology

product type :

antibody

product name :

GABRA1 Antibody (OAPC00118)

catalog :

OAPC00118

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs, bovine

application :

western blot

product information

sku :

OAPC00118

product type :

Polyclonal Antibody

category :

Polyclonal

name :

GABRA1 Antibody (OAPC00118)

size :

100 ul

gene symbol :

GABRA1

protein accession num :

NP_899155.1

swissprot id :

P62813

species reactivity :

Cow, Dog, Human, Mouse, Rat

product format :

Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

reconstitution and storage :

For long term storage -20C is recommended. Stable at -20C for at least 1 year.

description of target :

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alpha s, four beta s and four gamma s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 199; Whiting et al., 1999; Ogris et al., 24). Injection in oocytes or mammalian cell lines of cRNA coding for alpha - and beta -subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma -subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha -subunits of the receptor (McKernan et al., 2; Mehta and Ticku, 1998; Ogris et al., 24; PÃƒÂ¶ltl et al., 23).

host :

Rabbit

application :

homology :

Cow; Dog; Human; Mouse; Rat

purification :

Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

clonality :

Polyclonal

specificity :

Specific for the ~51k alpha 1-subunit of the GABAA receptor in Western blots. Labeling is absent in alpha 1-subunit knockout animals.

immunogen :

Fusion protein from the cytoplasmic loop of the alpha 1-subunit of rat GABAA receptor.

application info :

The antibody has been directly tested for reactivity in Western blots with mouse and rat tissue. It is anticipated that the antibody will react with bovine, canine, human, and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen.Western blot of mouse forebrain lysates from wild type (Control) and alpha 1-knockout (alpha 1-K/O) animals showing specific immunolabeling of the ~51k alpha 1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha 1-knockout animals.

drywet surcharge :

Wet Ice

storage temperature :

-20C