5-Methylcytosine Antibody (AMM99021) | Aviva Systems Biology AMM99021 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Aviva Systems Biology

product type :

antibody

product name :

5-Methylcytosine Antibody (AMM99021)

catalog :

AMM99021

quantity :

50 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

33D3

reactivity :

human, mouse

application :

immunocytochemistry, immunoprecipitation, flow cytometry, radioimmunoassay, chromatin immunoprecipitation

citations: 6

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:100; fig 3d	Sugawa F, AraÃºzo Bravo M, Yoon J, Kim K, Aramaki S, Wu G, et al. Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile. EMBO J. 2015;34:1009-24 pubmed publisher
immunocytochemistry; mouse	Tajbakhsh J, Stefanovski D, Tang G, Wawrowsky K, Liu N, Fair J. Dynamic heterogeneity of DNA methylation and hydroxymethylation in embryonic stem cell populations captured by single-cell 3D high-content analysis. Exp Cell Res. 2015;332:190-201 pubmed publisher
	Gell J, Zhao J, Chen D, Hunt T, Clark A. PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation. Stem Cell Res. 2018;27:46-56 pubmed publisher
	Gong Z, Xue C, Zhang M, Guo R, Zhou Y, Shi G. Physical localization and DNA methylation of 45S rRNA gene loci in Jatropha curcas L. PLoS ONE. 2013;8:e84284 pubmed publisher
	Yu J, Feng Q, Ruan Y, Komers R, Kiviat N, Bomsztyk K. Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays. BMC Mol Biol. 2011;12:49 pubmed publisher
	Vrba L, Junk D, Novak P, Futscher B. p53 induces distinct epigenetic states at its direct target promoters. BMC Genomics. 2008;9:486 pubmed publisher

product information

sku :

AMM99021

product type :

Monoclonal Antibody

category :

Monoclonal

name :

5-Methylcytosine Antibody (AMM99021)

size :

50 ug

species reactivity :

Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken

product format :

Liquid. PBS pH 7.4 (NaCl 137 mM, KCI 2.7 mM, 10 mM Na2HPO4, 2 mM KH2PO4). No preservative.

reconstitution and storage :

Ready for usage. For longer periods of storage, store at -20 C. Can be stored 1 month at 4 C.Avoid repeat freeze-thaw cycles.

description of target :

Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 6 to 9% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.

host :

Mouse

application :

ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF

purification :

Protein A ceramic hyper DF affinity chromatography

clonality :

Monoclonal

clone :

33D3

purity :

> 95% (Gel electrophoresis SDS PAGE. Densitometry analysis)

drywet surcharge :

Room Temperature

storage temperature :

-20C