This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Antibodies-online
product type :
antibody
product name :
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 25-60) antibody
catalog :
ABIN733208
quantity :
100 μL
price :
269.5 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry
product information
Catalog Number :
ABIN733208
Product Name :
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 25-60) antibody
Host Species :
Rabbit
Clonality :
Polyclonal
Antigen :
C-Raf/Raf1
Product Type :
Primary
Size :
100 μL
List Price USD :
269.5 USD
List Of Pubmed ID :
24589546
Image Legend :
Images provided the Independent Validation Program (badge number 29651)Formalin-fixed and paraffin embedded human colon labeled with Rabbit Anti-c-Raf/Raf1 Polyclonal Antibody (ABIN733208) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.
Isotype :
IgG
Product Description :
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. Subcellular location: Cytoplasm, Nucleus, Mitochondrion, Cell membrane Synonyms: NS5, CRAF, Raf-1, c-Raf, CMD1NN, RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, RAF1, RAF
Immunogen :
KLH conjugated synthetic peptide derived from human c Raf
Epitope :
AA 25-60
Reactivity :
Human,Mouse (Murine),Rat (Rattus)
Buffer :
Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Concentration :
1 μg/μL
Application Summary :
Immunofluorescence (Paraffin-embedded Sections) (IF (p)),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)),Western Blotting (WB)
Uniprot ID :
P04049
company information
Antibodies-online
Schloss-Rahe-Str. 15, 52072 Aachen
info@antibodies-online.com
http://www.antibodies-online.com
49 (0)241 95 163 153
headquarters: Germany
antibodies-online.com facilitates researchers to find the appropriate antibodies for their respective research project. As an independent and multi-vendor marketplace for research antibodies and thanks to the cooperation with more than 120 different manufacturers, we offer a large selection of Antibodies (over 500,000), ELISA Kits (over 200,000), Secondary Antibodies (13,000), Proteins (150,000), Peptides and Isotype Controls. In addition the scientists receive news and detailed information about the individual antibodies from databases such as the PubMed and others.
We help scientists to find the right products in our database. Similar products can be compared, our technical hotline offers consulting and the possibility to order directly with us.