This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Antibodies-online
product type :
antibody
product name :
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody
catalog :
ABIN400785
quantity :
25 μL
price :
253 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
17F6-B11
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, enzyme immunoassay
citations: 4
Reference
Lawlor M, Alessi D. PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?. J Cell Sci. 2001;114:2903-10 pubmed
Alessi D. Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture. Biochem Soc Trans. 2001;29:1-14 pubmed
Staal S. Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma. Proc Natl Acad Sci U S A. 1987;84:5034-7 pubmed
Jones P, Jakubowicz T, Pitossi F, Maurer F, Hemmings B. Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily. Proc Natl Acad Sci U S A. 1991;88:4171-5 pubmed
product information
Catalog Number :
ABIN400785
Product Name :
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody
Host Species :
Mouse
Clonality :
Monoclonal
Antigen :
AKT1 / PKB
Product Type :
Primary
Size :
25 μL
List Price USD :
253 USD
List Of Pubmed ID :
1851997,3037531,11686294,11356119
Clone :
17F6-B11
Isotype :
IgG1
Product Description :
AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphory-lates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Immunogen :
Synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein
Epitope :
Ser473
Reactivity :
Chimpanzee,Human,Mouse (Murine),Rat (Rattus)
Buffer :
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Concentration :
1.0 mg/mL (by UV absorbance at 280 nm)
Application Summary :
Enzyme Immunoassay (EIA),Immunofluorescence (IF),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)),Immunoprecipitation (IP),Western Blotting (WB)
Uniprot ID :
P31749
company information
Antibodies-online
Schloss-Rahe-Str. 15, 52072 Aachen
info@antibodies-online.com
http://www.antibodies-online.com
49 (0)241 95 163 153
headquarters: Germany
antibodies-online.com facilitates researchers to find the appropriate antibodies for their respective research project. As an independent and multi-vendor marketplace for research antibodies and thanks to the cooperation with more than 120 different manufacturers, we offer a large selection of Antibodies (over 500,000), ELISA Kits (over 200,000), Secondary Antibodies (13,000), Proteins (150,000), Peptides and Isotype Controls. In addition the scientists receive news and detailed information about the individual antibodies from databases such as the PubMed and others.
We help scientists to find the right products in our database. Similar products can be compared, our technical hotline offers consulting and the possibility to order directly with us.