anti-Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1) antibody | Antibodies-online ABIN263943 product information

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product summary

company name :

Antibodies-online

product type :

antibody

product name :

anti-Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1) antibody

catalog :

ABIN263943

quantity :

0.2 mg

price :

423.5 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

H90-10

reactivity :

human, rat, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, enzyme immunoassay, immunohistochemistry - frozen section

citations: 8

Reference
Arlander S, Eapen A, Vroman B, McDonald R, Toft D, Karnitz L. Hsp90 inhibition depletes Chk1 and sensitizes tumor cells to replication stress. J Biol Chem. 2003;278:52572-7 pubmed
Pearl L, Prodromou C. Structure, function, and mechanism of the Hsp90 molecular chaperone. Adv Protein Chem. 2001;59:157-86 pubmed
Loo M, Jensen T, Cui L, Hou Y, Chang X, Riordan J. Perturbation of Hsp90 interaction with nascent CFTR prevents its maturation and accelerates its degradation by the proteasome. EMBO J. 1998;17:6879-87 pubmed
Pratt W. The hsp90-based chaperone system: involvement in signal transduction from a variety of hormone and growth factor receptors. Proc Soc Exp Biol Med. 1998;217:420-34 pubmed
Barent R, Nair S, Carr D, Ruan Y, Rimerman R, Fulton J, et al. Analysis of FKBP51/FKBP52 chimeras and mutants for Hsp90 binding and association with progesterone receptor complexes. Mol Endocrinol. 1998;12:342-54 pubmed
Nemoto T, Sato N, Iwanari H, Yamashita H, Takagi T. Domain structures and immunogenic regions of the 90-kDa heat-shock protein (HSP90). Probing with a library of anti-HSP90 monoclonal antibodies and limited proteolysis. J Biol Chem. 1997;272:26179-87 pubmed
Pratt W, Toft D. Steroid receptor interactions with heat shock protein and immunophilin chaperones. Endocr Rev. 1997;18:306-60 pubmed
Minami Y, Kawasaki H, Miyata Y, Suzuki K, Yahara I. Analysis of native forms and isoform compositions of the mouse 90-kDa heat shock protein, HSP90. J Biol Chem. 1991;266:10099-103 pubmed

product information

Catalog Number :

ABIN263943

Product Name :

anti-Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1) antibody

Host Species :

Mouse

Clonality :

Monoclonal

Antigen :

HSP90AB1 / HSP90 beta

Product Type :

Primary

Size :

0.2 mg

List Price USD :

423.5 USD

List Of Pubmed ID :

9334185,2037568,14570880,11868271,9183567,9514152,9843494,9521088

Clone :

H90-10

Isotype :

IgG2a

Product Description :

HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms α and β, which share 85 % sequence amino acid homology. The two isoforms of Hsp90, are expressed in the cytosolic compartment (1). Despite the similarities, HSP90α exists predominantly as a homodimer while HSP90β exists mainly as a monomer.(2) From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. (3-6) Furthermore, Hsp90 is highly conserved between species, having 60 % and 78 % amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite it's label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1-2 % of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90-regulated proteins that have been discovered to date are involved in cell signaling. (7-8). The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (9).Synonyms: HSP84, HSP90B, HSPC2, HSPCB, Heat shock 84 kDa, Heat shock protein HSP 90-beta

Immunogen :

Recombinant human hsp90 beta.

Reactivity :

Chicken,Dog (Canine),Human,Rabbit,Rat (Rattus)

Buffer :

PBS pH 7.2 containing 50 % Glycerol, 0.09 % Sodium Azide

Concentration :

1.0 mg/mL

Application Summary :

Enzyme Immunoassay (EIA),Immunofluorescence (IF),Immunohistochemistry (Frozen Sections) (IHC (fro)),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)),Immunoprecipitation (IP),Western Blotting (WB)

Uniprot ID :

P08238