anti-Glial Fibrillary Acidic Protein (GFAP) (pSer13) antibody | Antibodies-online ABIN1449159 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Antibodies-online

product type :

antibody

product name :

anti-Glial Fibrillary Acidic Protein (GFAP) (pSer13) antibody

catalog :

ABIN1449159

quantity :

0.1 mg

price :

401.5 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

KT13

reactivity :

human

application :

western blot, immunocytochemistry

citations: 11

Reference
Kawajiri A, Yasui Y, Goto H, Tatsuka M, Takahashi M, Nagata K, et al. Functional significance of the specific sites phosphorylated in desmin at cleavage furrow: Aurora-B may phosphorylate and regulate type III intermediate filaments during cytokinesis coordinatedly with Rho-kinase. Mol Biol Cell. 2003;14:1489-500 pubmed
Takemura M, Gomi H, Colucci Guyon E, Itohara S. Protective role of phosphorylation in turnover of glial fibrillary acidic protein in mice. J Neurosci. 2002;22:6972-9 pubmed
Takemura M, Nishiyama H, Itohara S. Distribution of phosphorylated glial fibrillary acidic protein in the mouse central nervous system. Genes Cells. 2002;7:295-307 pubmed
Nagata K, Izawa I, Inagaki M. A decade of site- and phosphorylation state-specific antibodies: recent advances in studies of spatiotemporal protein phosphorylation. Genes Cells. 2001;6:653-64 pubmed
Yasui Y, Amano M, Nagata K, Inagaki N, Nakamura H, Saya H, et al. Roles of Rho-associated kinase in cytokinesis; mutations in Rho-associated kinase phosphorylation sites impair cytokinetic segregation of glial filaments. J Cell Biol. 1998;143:1249-58 pubmed
Inagaki M, Inagaki N, Takahashi T, Takai Y. Phosphorylation-dependent control of structures of intermediate filaments: a novel approach using site- and phosphorylation state-specific antibodies. J Biochem. 1997;121:407-14 pubmed
Kosako H, Amano M, Yanagida M, Tanabe K, Nishi Y, Kaibuchi K, et al. Phosphorylation of glial fibrillary acidic protein at the same sites by cleavage furrow kinase and Rho-associated kinase. J Biol Chem. 1997;272:10333-6 pubmed
MacAulay C, Forbes D. Assembly of the nuclear pore: biochemically distinct steps revealed with NEM, GTP gamma S, and BAPTA. J Cell Biol. 1996;132:5-20 pubmed
Inagaki N, Ito M, Nakano T, Inagaki M. Spatiotemporal distribution of protein kinase and phosphatase activities. Trends Biochem Sci. 1994;19:448-52 pubmed
Nishizawa K, Yano T, Shibata M, Ando S, Saga S, Takahashi T, et al. Specific localization of phosphointermediate filament protein in the constricted area of dividing cells. J Biol Chem. 1991;266:3074-9 pubmed
Matsuoka Y, Nishizawa K, Yano T, Shibata M, Ando S, Takahashi T, et al. Two different protein kinases act on a different time schedule as glial filament kinases during mitosis. EMBO J. 1992;11:2895-902 pubmed

product information

Catalog Number :

ABIN1449159

Product Name :

anti-Glial Fibrillary Acidic Protein (GFAP) (pSer13) antibody

Host Species :

Mouse

Clonality :

Monoclonal

Antigen :

GFAP

Product Type :

Primary

Size :

0.1 mg

List Price USD :

401.5 USD

List Of Pubmed ID :

8567730,12686604,12177195,11918673,11532025,9832553,9099667,9133607,7855885,1379174,1993680

Clone :

KT13

Isotype :

IgG1

Product Description :

Intermediate filaments (IFs) constitute major components of the cytoskeleton and the nuclear envelope in most cell types. Unlike other cytoskeletons such as microtubules and actin filaments, the protein components of IFs vary in a cell-, tissue-, and differentiation-dependent manner. Although IFs were thought to be relatively stable compared with actin filaments and microtubules, intensive in vitro investigations revealed that site-specific phosphorylation by several kinases, such as protein kinase A (PKA), protein kinase C (PKC), Ca2+/Calmodulin kinase II (CaMKII), cdc2 kinase, and Rho-kinase alters dynamically their structure and induces filament disruption. Glial fibrillary acidic protein (GFAP) belongs to IF proteins. GFAP is a useful marker of mature astrocytes and mutation in the GFAP gene has recently been associated with Alexander disease. Inagaki et al. reported that GFAP was phosphorylated specifically at Thr-7, Ser-13, and Ser-34 by Rho-kinase and Aurora-B and all of these sites were phosphorylated at the cleavage furrow during cytokinesis.Synonyms: Glial Fibrillary Acidic Protein

Immunogen :

KLH-pG13 peptide

Epitope :

pSer13

Reactivity :

Human

Buffer :

PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Concentration :

1.0 mg/mL

Application Summary :

Immunofluorescence (IF),Western Blotting (WB)

Uniprot ID :

P14136