anti-Vimentin (VIM) (pSer55) antibody | Antibodies-online ABIN1109485 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Antibodies-online

product type :

antibody

product name :

anti-Vimentin (VIM) (pSer55) antibody

catalog :

ABIN1109485

quantity :

0.1 mg

price :

401.5 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4A4

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

citations: 11

Reference
Tatsumi Y, Sugimoto N, Yugawa T, Narisawa Saito M, Kiyono T, Fujita M. Deregulation of Cdt1 induces chromosomal damage without rereplication and leads to chromosomal instability. J Cell Sci. 2006;119:3128-40 pubmed
Oguri T, Inoko A, Shima H, Izawa I, Arimura N, Yamaguchi T, et al. Vimentin-Ser82 as a memory phosphorylation site in astrocytes. Genes Cells. 2006;11:531-40 pubmed
Shiromizu T, Goto H, Tomono Y, Bartek J, Totsukawa G, Inoko A, et al. Regulation of mitotic function of Chk1 through phosphorylation at novel sites by cyclin-dependent kinase 1 (Cdk1). Genes Cells. 2006;11:477-85 pubmed
Kohn K, Aladjem M, Weinstein J, Pommier Y. Molecular interaction maps of bioregulatory networks: a general rubric for systems biology. Mol Biol Cell. 2006;17:1-13 pubmed
Tsai J, Chen Y, Kriegstein A, Vallee R. LIS1 RNA interference blocks neural stem cell division, morphogenesis, and motility at multiple stages. J Cell Biol. 2005;170:935-45 pubmed
Englund C, Fink A, Lau C, Pham D, Daza R, Bulfone A, et al. Pax6, Tbr2, and Tbr1 are expressed sequentially by radial glia, intermediate progenitor cells, and postmitotic neurons in developing neocortex. J Neurosci. 2005;25:247-51 pubmed
Miyata T, Kawaguchi A, Saito K, Kawano M, Muto T, Ogawa M. Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells. Development. 2004;131:3133-45 pubmed
Nakamura Y, Hashimoto R, Amano M, Nagata K, Matsumoto N, Goto H, et al. Localized phosphorylation of vimentin by rho-kinase in neuroblastoma N2a cells. Genes Cells. 2000;5:823-37 pubmed
Inada H, Togashi H, Nakamura Y, Kaibuchi K, Nagata K, Inagaki M. Balance between activities of Rho kinase and type 1 protein phosphatase modulates turnover of phosphorylation and dynamics of desmin/vimentin filaments. J Biol Chem. 1999;274:34932-9 pubmed
Goto H, Kosako H, Tanabe K, Yanagida M, Sakurai M, Amano M, et al. Phosphorylation of vimentin by Rho-associated kinase at a unique amino-terminal site that is specifically phosphorylated during cytokinesis. J Biol Chem. 1998;273:11728-36 pubmed
Scholey J. Kinesin-II, a membrane traffic motor in axons, axonemes, and spindles. J Cell Biol. 1996;133:1-4 pubmed

product information

Catalog Number :

ABIN1109485

Product Name :

anti-Vimentin (VIM) (pSer55) antibody

Host Species :

Mouse

Clonality :

Monoclonal

Antigen :

Vimentin

Product Type :

Primary

Size :

0.1 mg

List Price USD :

401.5 USD

List Of Pubmed ID :

16267266,8601599,16835273,16629905,16629900,16144905,15634788,15175243,11029658,10574968,9565595

Clone :

4A4

Isotype :

IgG2b

Product Description :

Vimentin is an intermediate filament protein distributed widely in the cytoplasm and is phosphorylated by several protein kinase in vitro. Ser55 residues on vimentin were reported to be one of the phosphorylation sites of vimentin at metaphase and were the phosphorylation sites for cdc2 kinase but not for cAMP-dependent protein kinase, protein kinase C, and Ca2+-calmodulin-dependent protein kinase II in vitro. Immunofluorescence and immunoelectron microscopy showed that vimentin Ser55 residues distributed in the entire cytoplasmic vimentin filament system are phosphorylated when the cells enter mitosis and de-phosphorylated in cytokinesis. The use of this antibody that specifically reacts with the phosphorylation site of vimentin Ser55 by cdc2 kinase enables estimation of a particular cdc2 kinase function.Synonyms: VIM

Immunogen :

Synthetic MPV55 phosphopeptide corresponding to mouse phosphorylated vimentin Ser55.

Epitope :

pSer55

Reactivity :

Human,Mouse (Murine),Rat (Rattus)

Buffer :

PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Concentration :

1.0 mg/mL

Application Summary :

Immunofluorescence (IF),Western Blotting (WB)

Uniprot ID :

P08670