American Research Products

protein

Hepatitis A Antigen (Grade 2)

12-3057

100 ul

99.00 USD

12-3057

Hepatitis A Antigen (Grade 2)

Recombinant Protein

100 ul

99.00 USD

Hepatitis A Virus (HAV) is a non-enveloped icosahedral virion with a single stranded RNA genome. Only one serotype of HAV is known and it does not cross react with Hepatitis B virus. The virus contains four proteins, VP1, VP2, VP3 and VP4. Optimally infected cells collected by centrifugation and the pelleted cells are resuspended in glycine buffer and the cells are disrupted by sonication to release the viral particles. The cellular debris is removed by low speed centrifugation. The clarified cell lysate is pooled and further purified by freon extraction. This antigen can be used “as is” in a variety of proven immunoassay procedures or may be further purified for specific applications.

Clarified cellular extracts from infected human diploid lung cells in Glycine, pH 9.5

Concentration may vary with each lot

Antigen in ELISA. Sonicate before use. HAV grade 2 antigen is inactivated by incubating with formaldehyde for 72 hours.We are aware of no specific hazards associated with this product. The reagent has been inactivated and should contain no infectious material. Generally accepted good laboratory practices appropriate to biological reagents should be employed when handling this product.

- 70°C, Avoid freeze and thaw cycles