American Research Products

protein

CCL3, 24-92aa, Human, His tag, E.coli, Recombinant

11-CCL-3HT

10 ug

189.00 USD

11-CCL-3HT

CCL3, 24-92aa, Human, His tag, E.coli, Recombinant

Recombinant Protein

10 ug

189.00 USD

CCL3, also known as, macrophage inflammatory protein-1alpha (MIP-1alpha), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This protein has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1beta and RANTES. It acts as an inhibitor of different strains of HIV-1, HIV-2 and SIV infection in a dose-dependent manner. Recombinant human CCL3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

> 90% by SDS - PAGE

Liquid. In 10mM Sodium Citrate ( pH3.5) containing 10% glycerol

0.25 mg/ml (determined by Bradford assay)

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Guan E, et al. (2001) J Biol Chem. 276:12404�9Cocchi F., et al. (1995) Science. 270:1811�5.