American Research Products

protein

CCL1, 24-96aa, Human, His tag, E.coli, Recombinant

11-CCL-1HT

10 ug

189.00 USD

11-CCL-1HT

CCL1, 24-96aa, Human, His tag, E.coli, Recombinant

Recombinant Protein

10 ug

189.00 USD

CCL1 (chemokine C-C motif ligand 1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. Unlike the other members of the C-C family, CCL1 does not induce chemotaxis in natural killer (NK) cells. Recombinant human CCL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

> 85% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 7.5) containing 2mM DTT, 10% glycerol, 50mM NaCl

0.5 mg/ml (determined by Bradford assay)

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Miller M.D., et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:2950-2954Roos RS, et al. (1997) J. Biol. Chem. 272(28): 17251�4.