American Research Products

antibody

Anti-CD44-Affinity Purified

08-9407-2

50 ug

199.00 USD

monoclonal

mouse

nonconjugated

western blot, ELISA

08-9407-2

Anti-CD44-Affinity Purified

Mouse

monoclonal

Purified

Mouse monoclonal

50 ug

199.00 USD

BRIC 235

Mouse IgG2b kappa light chain

Mouse monoclonal antibody to human CD44 Affinity purified. CD44 (also known as Pgp-1, ECMR III, Hermes antigen, p801, H- CAM) is a cell membrane glycoprotein of apparent molecular weight 80 kDa. The full amino acid sequence has been deduced from cDNA. It is heavily glycosylated with both N- and O- glycans. The extracellular part of CD44 comprises an N- terminal disulphide bonded domain and an O- glycosylated domain. CD44 carries the Ina and Inb blood group antigens2. There is a strong association between CD44 and the cytoskeleton. CD44 is thought to be involved in mediating cell:cell adhesion particularly lymphocyte- endothelial cell interactions important for lymphocyte migration from blood to lymph nodes and mucosal associated lymph organs. CD44 is a member of the hyaladherin family of hyaluronan-binding proteins, with a structure similar to selectins, and is the principal cell surface receptor for Hyaluronate3. Antibodies in CD44 may facilitate haemopoietic engraftment4. CD44 also functions as an adhesion, hyaluronan, fibronectin, osteopontin and MIP-1b receptor and as a co-stimulatory molecule. CD44 is found on a broad range of haemopoietic cells such as lymphoid cells, myeloid cells, fibroblasts, endothelial cells, epithelial cells, erythroid cells and the nervous system, but not platelets5. It is found on brain, heart, liver, thymus, kidney and colon epithelium. CD44 has been mapped to chromosome 11p13. There are approximately 10,000 CD44 molecules per erythrocyte.

BRIC 235 reacts by immunoblotting with a component of Mr 80kDa in nonreduced erythrocyte membranes. Epitope mapping correlates BRIC 222 with the Hermes 2 group of CD44 antibodies which is equivalent to epitope 16. This epitope appears to be associated with the N- terminal region of CD44. It inhibits T cell:erythrocyte rosette formation. BRIC 222 is a direct haemagglutinin. The erythrocyte antigen is pronase, rypsin, chymotrypsin and AET (2- aminoisothiouronium bromide) sensitive.

Immunoblotting, ELISA

2-8°C for immediate use, or at -20°C (aliquot)

1. Haynes, B.F. et al., (1989) Immunol. Today 10: 423-428 (Review).2. Spring, F.A., et al. (1988) Immunology 64: 37-433. Aruffo, A., et al. (1990) Cell 61: 1303-13134. Sandmaier, B.M. et al. (1990) 76: 630-6355. Stoll, M. et al. (1989), in Leucocyte typing IV; White cell Differentiation Antigens, Ed. W. Knapp et al., Oxford University Press,pp 619-6226. Anstee, DJ et al. (1991) Immunology 74: 197-2057. Spring FA et al. (1993) Proceedings of the fifth workshop and conference on white cell differentiation antigens, Boston 2: 738-17408. Liao H, Telen M.J. Haynes B.F. (1993) Proceedings of the fifth workshop and conference on white cell differentiation antigens, Boston, vol. 2 p1735-7