Anti-CD58 (LFA-3)-Affinity Purified | American Research Products 08-9405-2 product information

product summary

company name :

American Research Products

product type :

antibody

product name :

Anti-CD58 (LFA-3)-Affinity Purified

catalog :

08-9405-2

quantity :

50 ug

price :

199.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot

more info or order :

American Research Products product webpage

product information

Catalog Number :

08-9405-2

Product Name :

Anti-CD58 (LFA-3)-Affinity Purified

Host Species :

Mouse

Clonality :

monoclonal

Conjugation :

Purified

Product Type :

Mouse monoclonal

Size :

50 ug

List Price :

199.00 USD

Clone :

BRIC 5

Isotype :

Mouse IgG2a kappa light chain

Product Description :

Mouse monoclonal antibody to human CD58 (LFA-3) Affinity purified. CD58, or LFA3, is a membrane glycoprotein of 55kDa to 70kDa and is mapped to chromosome 1p13. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes and natural killer cells1. It is a member of the immunoglobulin superfamily of molecules. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, fibroblasts, epithelium and endothelium cells2. There are approximately 5000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on cells of individuals with paroxysmal nocturnal haemoglobinuria.

Specificity :

BRIC 5 reacts with epitope cluster 1 defined by the fifth leucocyte workshop3 and will cross block TS2/94. It binds to a broadly migrating component of 40-65 KDa on an immunoblot of non-reduced erythrocyte membranes. BRIC 5 is an indirect haemagglutinin. The erythrocyte antigen is pronase, trypsin, chymotrypsin and AET sensitive. CD58 was clustered for the first time at the fourth leucocyte workshop2 by three antibodies including BRIC 5.

Application Summary :

It reacts by immunoblotting to non-reduced erythrocyte membranes

Storage :

2-8°C for immediate use, or at -20°C (aliquot)

General References :

1. Makgoba M.W., et al, (1989) Immunology Today 10 417-422 (Review)2. Shaw S. & Johnson J.P., (1989) in Leucocyte Typing IV; White Cell Differentiation Antigens Ed. W. Knapp et al Oxford University Press pp 714-7163. Klickstein et al., (1993) Proceedings of the fifth workshop and conference on white cell differentiation antigens, Boston, vol. 2 1475-1476.4. Anstee DJ et al. (1991) Immunology, 74: 197-205.5. Schlossman SF (ed) et al., (1993) Proceedings of the fifth workshop and conference on white cell differentiation antigens, Boston, Oxford University Press 1995.

more info or order :

American Research Products product webpage