American Research Products

antibody

Anti-N antigen-Culture Supernatant

08-9401-1

0.5 ml

109.00 USD

monoclonal

mouse

nonconjugated

western blot, ELISA

08-9401-1

Anti-N antigen-Culture Supernatant

Mouse

monoclonal

Purified

Mouse monoclonal

0.5 ml

109.00 USD

BRIC 157

Mouse IgG1kappa light chain

Mouse monoclonal antibody to human N antigen-Culture Supernatant. The blood group N antigen is expressed on the erythrocyte sialoglycoproteins Glycophorin A (GPA, where it is polymorphic, CD 235a) and Glycophorin B (GPB, CD 235b). The complete amino acid sequence of both of these highly homologous membrane glycoproteins is known. The nature of the amino acids at positions 1 and 5 (leucine and glutamic acid respectively) defines the N antigen on both GPA and GPB1. Glycophorins A and B are heavily glycosylated with numerous O- glycans containing sialic acid. The reactivity of some anti-N reagents is affected by the degree of sialylation of the antigen2. The antigen is found on erythroid cells and K562 and HEL erythroleukaemia cell lines. All normal erythrocytes express N antigen on GPB: MN and NN erythrocytes also express N antigen on GPA. There are approximately 2-10 x 105 GPA and 8-25 x 104 GPB molecules per erythrocyte. Rare individuals lacking GPA and GPB are known2.

Human N-antigen. The antibody has been used together with anti-M (6A7, 08-9412-) to develop a mutagenesis assay capable of quantitating the level of mutant M-N- red cells in normal and pathological samples3. BRIC 157 (anti-N formalin resistant epitope) reacts well with erythrocytes in a fixed specimen of MN blood group bone marrow

ELISA, Immunoblotting

2-8°C for immediate use, or at -20°C (aliquot)

1. Reid ME, Lomas-Francis C. (1997) The blood group antigen facts book. Academic Press, London2. Anstee, D.J., (1990) Vox Sang; 58: 1 20 (Review).3. Langlois R.G. et al.,(1990) Cytometry 11: 513-521