product summary
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company name :
American Research Products
product type :
antibody
product name :
Anti-p62 Protein (SQSTM1), C-Terminal Specific
catalog :
03-GP62-C
quantity :
25 ul
price :
169.00 USD
clonality :
polyclonal
host :
guinea pigs
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 32
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:100; loading ...; fig 4b
Watanabe S, Komine O, Endo F, Wakasugi K, Yamanaka K. Intracerebroventricular administration of Cystatin C ameliorates disease in SOD1-linked amyotrophic lateral sclerosis mice. J Neurochem. 2018;145:80-89 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1d
Hammerling B, Najor R, Cortez M, Shires S, Leon L, Gonzalez E, et al. A Rab5 endosomal pathway mediates Parkin-dependent mitochondrial clearance. Nat Commun. 2017;8:14050 pubmed publisher
  • western blot; rat; fig 4a
Bartlett J, Trivedi P, Yeung P, Kienesberger P, Pulinilkunnil T. Doxorubicin impairs cardiomyocyte viability by suppressing transcription factor EB expression and disrupting autophagy. Biochem J. 2016;473:3769-3789 pubmed
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 4c
Walker W, Oehler A, Edinger A, Wagner K, Gunn T. Oligodendroglial deletion of ESCRT-I component TSG101 causes spongiform encephalopathy. Biol Cell. 2016;108:324-337 pubmed publisher
  • western blot; human; 1:1000; fig 2
Gschweitl M, Ulbricht A, Barnes C, Enchev R, Stoffel Studer I, Meyer Schaller N, et al. A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes. elife. 2016;5:e13841 pubmed publisher
  • western blot; mouse; loading ...; fig s5d
Liu Y, Takahashi Y, Desai N, Zhang J, Serfass J, Shi Y, et al. Bif-1 deficiency impairs lipid homeostasis and causes obesity accompanied by insulin resistance. Sci Rep. 2016;6:20453 pubmed publisher
  • western blot; mouse; fig 6
  • western blot; human; fig 6
Draganov D, Gopalakrishna Pillai S, Chen Y, Zuckerman N, Moeller S, Wang C, et al. Modulation of P2X4/P2X7/Pannexin-1 sensitivity to extracellular ATP via Ivermectin induces a non-apoptotic and inflammatory form of cancer cell death. Sci Rep. 2015;5:16222 pubmed publisher
  • immunocytochemistry; mouse; 1:1000
Kett L, Stiller B, Bernath M, Tasset I, Blesa J, Jackson Lewis V, et al. α-Synuclein-independent histopathological and motor deficits in mice lacking the endolysosomal Parkinsonism protein Atp13a2. J Neurosci. 2015;35:5724-42 pubmed publisher
  • western blot; mouse; fig 2
Lin H, Lin S, Chung Y, Vonderfecht S, Camden J, Flodby P, et al. Dynamic involvement of ATG5 in cellular stress responses. Cell Death Dis. 2014;5:e1478 pubmed publisher
  • western blot; human; 1:1700
Zhong Y, Morris D, Jin L, Patel M, Karunakaran S, Fu Y, et al. Nrbf2 protein suppresses autophagy by modulating Atg14L protein-containing Beclin 1-Vps34 complex architecture and reducing intracellular phosphatidylinositol-3 phosphate levels. J Biol Chem. 2014;289:26021-37 pubmed publisher
  • immunohistochemistry; mouse; 1:1000
  • western blot; mouse; 1:1000
Lu J, He L, Behrends C, Araki M, Araki K, Jun Wang Q, et al. NRBF2 regulates autophagy and prevents liver injury by modulating Atg14L-linked phosphatidylinositol-3 kinase III activity. Nat Commun. 2014;5:3920 pubmed publisher
  • immunohistochemistry; rat; 1:200
  • western blot; rat; 1:200
Yang S, Xia C, Li S, Du L, Zhang L, Hu Y. Mitochondrial dysfunction driven by the LRRK2-mediated pathway is associated with loss of Purkinje cells and motor coordination deficits in diabetic rat model. Cell Death Dis. 2014;5:e1217 pubmed publisher
  • western blot; human; fig 2
Lee I, Yun J, Finkel T. The emerging links between sirtuins and autophagy. Methods Mol Biol. 2013;1077:259-71 pubmed publisher
  • western blot; mouse; 1:1000; fig 9a
Zhou J, Freeman T, Ahmad F, Shang X, Mangano E, Gao E, et al. GSK-3? is a central regulator of age-related pathologies in mice. J Clin Invest. 2013;123:1821-32 pubmed publisher
Raso F, Sagadiev S, Du S, Gage E, Arkatkar T, Metzler G, et al. αv Integrins regulate germinal center B cell responses through noncanonical autophagy. J Clin Invest. 2018;128:4163-4178 pubmed publisher
Pan H, Yan Y, Liu C, Finkel T. The role of ZKSCAN3 in the transcriptional regulation of autophagy. Autophagy. 2017;13:1235-1238 pubmed publisher
Serfass J, Takahashi Y, Zhou Z, Kawasawa Y, Liu Y, Tsotakos N, et al. Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection. J Biol Chem. 2017;292:10097-10111 pubmed publisher
Dower C, Bhat N, Wang E, Wang H. Selective Reversible Inhibition of Autophagy in Hypoxic Breast Cancer Cells Promotes Pulmonary Metastasis. Cancer Res. 2017;77:646-657 pubmed publisher
Yap C, Digilio L, McMahon L, Roszkowska M, Bott C, Kruczek K, et al. Different Doublecortin (DCX) Patient Alleles Show Distinct Phenotypes in Cultured Neurons: EVIDENCE FOR DIVERGENT LOSS-OF-FUNCTION AND "OFF-PATHWAY" CELLULAR MECHANISMS. J Biol Chem. 2016;291:26613-26626 pubmed publisher
Zhang J, Tripathi D, Jing J, Alexander A, Kim J, Powell R, et al. ATM functions at the peroxisome to induce pexophagy in response to ROS. Nat Cell Biol. 2015;17:1259-1269 pubmed publisher
Tanaka Y, Chambers J, Matsuwaki T, Yamanouchi K, Nishihara M. Possible involvement of lysosomal dysfunction in pathological changes of the brain in aged progranulin-deficient mice. Acta Neuropathol Commun. 2014;2:78 pubmed publisher
Liu X, Yao J, Tripathi D, Ding Z, Xu Y, Sun M, et al. Autophagy mediates HIF2α degradation and suppresses renal tumorigenesis. Oncogene. 2015;34:2450-60 pubmed publisher
Hariharan N, Ikeda Y, Hong C, Alcendor R, Usui S, Gao S, et al. Autophagy plays an essential role in mediating regression of hypertrophy during unloading of the heart. PLoS ONE. 2013;8:e51632 pubmed publisher
Sishi B, Loos B, van Rooyen J, Engelbrecht A. Autophagy upregulation promotes survival and attenuates doxorubicin-induced cardiotoxicity. Biochem Pharmacol. 2013;85:124-34 pubmed publisher
Inoue K, Rispoli J, Kaphzan H, Klann E, Chen E, Kim J, et al. Macroautophagy deficiency mediates age-dependent neurodegeneration through a phospho-tau pathway. Mol Neurodegener. 2012;7:48 pubmed publisher
Lin T, Chen Y, Kensicki E, Li A, Kong M, Li Y, et al. Autophagy: resetting glutamine-dependent metabolism and oxygen consumption. Autophagy. 2012;8:1477-93 pubmed publisher
Young M, Takahashi Y, Khan O, Park S, Hori T, Yun J, et al. Autophagosomal membrane serves as platform for intracellular death-inducing signaling complex (iDISC)-mediated caspase-8 activation and apoptosis. J Biol Chem. 2012;287:12455-68 pubmed publisher
Kovacs J, Li C, Yang Q, Li G, Garcia I, Ju S, et al. Autophagy promotes T-cell survival through degradation of proteins of the cell death machinery. Cell Death Differ. 2012;19:144-52 pubmed publisher
Luo C, Li B, Li Q, Chen X, Sun Y, Bao H, et al. Autophagy is involved in traumatic brain injury-induced cell death and contributes to functional outcome deficits in mice. Neuroscience. 2011;184:54-63 pubmed publisher
Lee J, Park S, Takahashi Y, Wang H. The association of AMPK with ULK1 regulates autophagy. PLoS ONE. 2010;5:e15394 pubmed publisher
Hariharan N, Zhai P, Sadoshima J. Oxidative stress stimulates autophagic flux during ischemia/reperfusion. Antioxid Redox Signal. 2011;14:2179-90 pubmed publisher
BAERGA R, Zhang Y, Chen P, Goldman S, Jin S. Targeted deletion of autophagy-related 5 (atg5) impairs adipogenesis in a cellular model and in mice. Autophagy. 2009;5:1118-30 pubmed
product information
Catalog Number :
03-GP62-C
Product Name :
Anti-p62 Protein (SQSTM1), C-Terminal Specific
Host Species :
Guinea Pig
Clonality :
polyclonal
Conjugation :
Purified
Product Type :
Guinea pig Polyclonal
Size :
25 ul
List Price :
169.00 USD
Product Description :
Guinea pig polyclonal to p62 Protein, C-Terminal Specific. Human 62 kD (p62) protein, present in intracytoplasmic inclusions (e.g. hyaline bodies) of hepatocellular carcinoma. P62 protein has been found in many tissues and cells, including lymphoid cells, serving probably a common cellular signal transduction mechanism. The antiserum stains also neurofibrillary tangles in the brain of patients suffering fromAlzheimer’s disease. Note: The p62 antigen might be susceptible to proteolytic degradation. We recommend to use protease inhibitors during sample preparation Synonyms: SQSTM1, Sequestosome 1, Ubiquitin-binding protein
Immunogen :
C-terminal domain (20 amino acids: C-NYD IGA ALD TIQ YSK HPP PL) of human p62 protein, coupled with KLH. This peptide sequence is identical in human, monkey, bovine, mouse, and rat
Specificity :
Human 62 kD (p62) protein, present in intracytoplasmic inclusions (e.g. hyaline bodies) of hepatocellular carcinoma. P62 protein has been found in many tissues and cells, including lymphoid cells, serving probably a common cellular signal transduction mechanism. The antiserum stains also neurofibrillary tangles in the brain of patients suffering from Alzheimer’s disease. Note: The p62 antigen might be susceptible to proteolytic degradation. We recommend to use protease inhibitors during sample preparation.
Application Summary :
Immunohistochemistry on frozen and paraffin sections (enhanced after microwave treatment) Cytological material Immunoblotting
Storage :
2-8°C
General References :
Stumptner, C. et al. (1999) Am. J. Pathol. 154: 1701-1710Janig, E. et al. (2005) Eur J Cell Biol. 84: 329-339Kuusisto, E. et al. (2007) Neuropathology & Applied Neurobiology, pp 1-12Aishima S et al. (2010) Am J Clin Pathol 134: 457-465Itakura E et al. (2011) J Cell Bio 192: 17-27
company information
American Research Products
135 Beaver Street, Suite 204
Waltham, MA 02452
orders@arp1.com
http://www.arp1.com
(800) 832-2611
headquarters: USA