American Research Products

antibody

Anti-Perilipin, N-terminal specific

03-GP29

25 ul

189.00 USD

polyclonal

guinea pigs

nonconjugated

western blot, immunohistochemistry

03-GP29

Anti-Perilipin, N-terminal specific

Guinea Pig

polyclonal

Purified

Guinea pig Polyclonal

25 ul

189.00 USD

Guinea pig polyclonal to Perilipin, N-terminal specific. Perilipins build a family of phosphoproteins. The predominant forms in adipocytes, perilipin A and B arise by alternative RNA splicing from a single gene, generating polypeptides of 57 and 46 kD, respectively. The N-terminus, however, remains unchanged.

Duplicated N-terminus of perilipin (aa 1-20; cf. Greenberg et al. 1992, JBC 266, 11341-11346)

The antiserum reacts specifically with perilipins (A and B) located at the surface of intracellular storage lipid droplets present e.g. in the adrenal gland, adipocytes of white and brown adipose tissue and cultured cells such as 3T3-L1 adipocytes and cultured steroidogenic adrenal cortical and Leydig cells. It also is a useful pathological marker since perilipin becames expressed de novo in hepatocyte steatogenesis. The antiserum does not cross-react with ADRP (adipophilin) or TIP47 proteins (additional members of the PAT-family).

Immunohistochemistry, suitable for frozen & paraffin embedded tissue after microwave treatment, Immunoblotting (Western), Cytological material

2-8°C

Heid HW et al. (1998) Cell Tissue Res 294: 309-321Ryden M et al. (2004) Biochem Biophys Res Comm 318: 168-175Forcheron F et al. (2005) Aeterioscler Thromb Vasc Biol 25: 1-7Yamaguchi T et al. (2006) Mol Cell Biochem 284: 167-173Straub BK et al. (2008) Hepatology 47: 1936-1946Straub BK et al. (2010) Modern Pathology 23: 480-492