American Research Products

antibody

Anti-Drebrin

03-GP254

25 ul

189.00 USD

polyclonal

guinea pigs

nonconjugated

03-GP254

Anti-Drebrin

Guinea Pig

polyclonal

Purified

Guinea pig Polyclonal (gp dreb254.2)

25 ul

189.00 USD

Guinea pig polyclonal to Drebrin.

Synthetic human peptide (aa 254-272) conjugated to KLH

The antiserum specifically reacts with drebrin, a widespread actin-associating protein of 70 kD (SDS-PAGE mobility shows Mr 120,000). Drebrin has originally been described only in neuronal cells, but has been recently found also in the actin-rich lamellipodia and filopodia of motile cells. It is enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells. Positive staining was found in many tissues, including diverse epithelia and carcinoma (e.g. in epidermis basal cells are positive, whereas overlying cells are negative; also positive: regenerating epithelium during wound healing; basal cell carcinoma; skin melanoma), specific types of endothelia and smooth muscle; especially prominent in the mesangial cells of renal glomeruli and in the Sertoli cells of testis. Also positive: epithelia of hair follicles and eccrine sweat glands. Consistently negative were, however, hepatocytes and cross-striated muscle

Immunohistochemistry on frozen (paraformaldehyde fixed) and paraffin (microwave treatment) sections Immunofluorescence microscopy Immunoblotting (western, ECL) Immunoprecipitation Cytological material

2-8°C

Peitsch WK. et al. (1999) Eur J Cell Biol 78: 767-778Peitsch WK. (2001) Eur J Cell Biol 80: 567-579Peitsch WK. et al. (2005) J Invest Dermatol 125: 761-774