protein

Receptor-Associated Protein (RAP), Rat

03-62321

500 ug

1,039.00 USD

Receptor-Associated Protein (RAP), Rat

Antigen

500 ug

1,039.00 USD

Rat his: RAP: c-myc fusion protein. Recombinant Rat Receptor-Associated Protein (RAP)

> 95%, SDS-PAGE

Lyophilized, salt balanced. Reconstitution: When reconstituted with 500ul H20 dist, solution will contain 1 mg/ml rat RAP protein in TBS, pH 7.5, 0.1% BSA, 0.09% Sodium Azide. Special note: Ligand binding to RAP is Ca2+ dependent and e.g. lipid receptors can be released from RAP by a buffer containing 10 mM EDTA (cf. Bajari et al. 2005, see ref. below). Furthermore, buffers containing phosphate should be avoided (it would form precipitates with Ca2+)

Protein standard in 1D and 2D SDS gelelectrophoresis, Immunoassays, Immunization

4°C (lyophilized); at -20°C (solution, aliquot)

Bajari TM, er al. Biol Chem 379, 1053-1062 (1998), Hayashi H, et al. J Biol Chem 279, 14009-14015 (2004), Bajari TM. et al. Methods 36, 109-116 (2005