Anti-Vimentin | American Research Products 03-61013 product information

product summary

company name :

American Research Products

product type :

antibody

product name :

Anti-Vimentin

catalog :

03-61013

quantity :

200 ul

price :

129.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA

more info or order :

American Research Products product webpage

product information

Catalog Number :

03-61013

Product Name :

Anti-Vimentin

Host Species :

Mouse

Clonality :

monoclonal

Conjugation :

Purified

Product Type :

Mouse monoclonal

Size :

200 ul

List Price :

129.00 USD

Clone :

VIM3B4

Isotype :

IgG2a

Product Description :

The antibody is highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. VIM 3B4 has turned out to be the most avid mab to vimentin. Polypeptide reacting: Mr 57 000 intermediate filament protein (vimentin) of mesenchymal cells. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma.The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).

Immunogen :

Vimentin (purified from bovine lens)

Application Summary :

Immunoblotting, ELISA, immunofluorescence microscopy, immunohistochemistry. Suitable for frozen and paraffin-embedded tissue and cytological material

Storage :

2-8°C for immediate use, or at -20°C (aliquot)

General References :

Franke, W.W. and Moll, R. (1987).:Differentiation 36: 145-163Heid, H.W. et al. (1988). Differentiation 37: 137-157Jahn, L.et al. (1987). Differentiation 36: 234-254Kasper, M.et al. (1989). Differentiation 40: 207-214Kasper, M.et al. (1989). Histochemistry 93: 93-103Mol, I. & Moll, R. (1991) Differentiation 40: 207-214Rungger-Braendle, E. et al. (1989) J Cell Biol. 109: 705-716Gomi, H. et al. (1995) Neuron 14: 29-41Demirkesen , C. et al. (1995) J. Cutan Pathol 22: 518-535Hermann, H. et al. (1993) J. Mol. Biol 234: 99-113Rogers, KR et al. (1995) Eur J Cell Biol 66: 136-150Bohn, W. et al. (1992) Exp. Cell Res 201: 1-7Hermann, H. et al. (1992) J. Mol Biol 223: 637-650

more info or order :

American Research Products product webpage