Anti-Adipophilin / ADRP | American Research Products 03-610102 product information

product summary

company name :

American Research Products

product type :

antibody

product name :

Anti-Adipophilin / ADRP

catalog :

03-610102

quantity :

200 ul

price :

129.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot

more info or order :

American Research Products product webpage

citations: 1

Reference
Shepherd S, Cocks M, Tipton K, Ranasinghe A, Barker T, Burniston J, et al. Sprint interval and traditional endurance training increase net intramuscular triglyceride breakdown and expression of perilipin 2 and 5. J Physiol. 2013;591:657-75 pubmed publisher

product information

Catalog Number :

03-610102

Product Name :

Anti-Adipophilin / ADRP

Host Species :

Mouse

Clonality :

monoclonal

Conjugation :

Purified

Product Type :

Mouse monoclonal

Size :

200 ul

List Price :

129.00 USD

Clone :

AP 125

Isotype :

IgG1

Product Description :

Mouse Monoclonal Antibody to Human homologue to mouse adipocyte differentiation-related protein (ADRP). Adipophilin / ADRP is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (see e.g. Heid et al.; for review see e.g. Targett- Adams et al.); inducible by etomoxir.Enhanced expression of Adipophilin / ADRP is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study virus entry via lipid droplets (see e.g. Hope et al., Samsa et al.).Polypeptide reacting: Adipophilin / ADRP, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72

Immunogen :

Synthetic peptide (aa 5-27 from N-terminus of human adipophilin)

Specificity :

Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative.Also positively stained are lipid-storing CD 68-positive macrophages.

Application Summary :

Immunoblotting (Western blot)Immunohistochemistry of frozen tissue (for staining protocols see ref. Ohsaki et al.)Paraffin-embedded tissue after microwave treatment (for stainingprotocol see Straub et al. 2008), Cytological material

Storage :

2-8°C for immediate use, or at -20°C (aliquot)

General References :

Heid, H.W.et al. (1997) (submitted for publication)Heid, HWet al. (1996) Biochem J. 320: 1025-1030*Steiner, S. et al. (1996). Biochem. Biophys. Res. 218: 777-778Mrozinzki, J et al. (1999) J. Cancer Res&Clin. Oncol. 125 supplementThan, NG et al. (1999) Tumor Biol. 20: 184-192Hope, RG et al. (2000) J. General Virol. 81: 1913-1925Ostermeyer, AG. Et al.(2001) J. Cell Biol. 152: 1071-1078Fujimoto, T. et al. (2001) j. Cell Biol. 152: 1079-1085Buechler, C. et al. (2001) Biochim Biophys Acta 1532: 97-104

more info or order :

American Research Products product webpage