American Research Products

protein

NEDD8, 1-76 aa, Human, His tagged, Recombinant, E.coli

01-2015

10 ug

159.00 USD

01-2015

NEDD8, 1-76 aa, Human, His tagged, Recombinant, E.coli

Recombinant Protein

10 ug

159.00 USD

NEDD8 belongs to the ubiquitin family and human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Recombinant human NEDD8 protein was expressed in E.coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.

> 95% by SDS - PAGE

In 20 mM Tris-HCl buffer (pH 8.0) containing 0.5mM DTT, 50mM NaCl, 10% glycerol

0.5 mg/ml (determined by Bradford assay)

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Wu K., et al. (2002) J Biol Chem. 277(1): 516-27Hori T., et al. (1999) Oncogene. 18(48): 6829-34