American Research Products

protein

Fumarase, 44-510aa, Human, Recombinant, E.coli

01-2010

20 ug

169.00 USD

01-2010

Fumarase, 44-510aa, Human, Recombinant, E.coli

Recombinant Protein

20 ug

169.00 USD

Fumarase (Fumarate hytdratase) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to S-malate and is involved in the tricarboxylic acid (TCA), or Krebs cycle. This enzyme exists in both a cytosolic form and an N-terminal extended mitochondrial form. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension is the same form as in the cytoplasm. Fumarase deficiency can lead to progressive encephalopathy, cerebral atrophy and developmental delay and this enzyme also is thought to act as a tumor suppressor. Recombinant Fumarase was expressed in E.coli and was purified by conventional chromatography techniques.

> 95% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH 8.0)

1 mg/ml (determined by Bradford assay)

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Lehtonen R., et al. (2003) J. Med. Genet. 40 (3): e19.Toro JR., et al. (2003) Am. J. Hum. Genet. 73(1): 95�106.