American Research Products

protein

ASF1A, 1-204aa, Human, His-tagged, Recombinant, E.coli

01-2007

10 ug

179.00 USD

01-2007

ASF1A, 1-204aa, Human, His-tagged, Recombinant, E.coli

Recombinant Protein

10 ug

179.00 USD

Histone chaperone ASF1A, CCG1-interacting factor A (CIA), CGI-98, HSPC146 Description ASF1 anti-silencing function 1 homolog A, also known as ASF1A, belongs to H3/H4 family of histone chaperone proteins. ASF1A protein interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. Deletion of ASF1A in yeast and Drosophila confers sensitivity to various DNA damaging agents and inhibitors of DNA replication, increases genomic instability and sister chromatid exchange, and activates the DNA damage checkpoint. Recombinant human ASF1A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

> 90% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT , 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Sanematsu F., et al. (2006). J Biol Chem. 281(19):13817-27Groth A., et al. (2005). Mol Cell. 17(2):301-11